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anti-Human Caspase 9 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Caspase 9 Antibody (supplied by: Log in to see )

Antigen
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
  • AI115399
  • APAF-3
  • APAF3
  • Apaf3
  • AW493809
  • CASP-9
  • Casp-9-CTD
  • CASP9
  • Casp9_v1
  • Caspase-9
  • Ice-Lap6
  • ICE-LAP6
  • MCH6
  • Mch6
  • PPP1R56
Reactivity
Human
413
119
116
19
13
9
6
5
4
3
3
2
1
1
1
1
Host
Rabbit
420
46
1
Clonality
Polyclonal
Conjugate
This Caspase 9 antibody is un-conjugated
11
10
7
4
4
4
4
4
4
4
4
4
3
3
3
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
390
161
128
77
68
68
45
34
13
13
8
6
2
2
2
Supplier
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Catalog No. ABIN498253
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.342316 ABIN498192 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.342316 ABIN756922 IF (p) IHC (p) WB Rabbit IgG AA 180-220, pSer196 Log in to see Polyclonal
0.342316 ABIN462358 IHC (p) IP WB Mouse IgG1 Log in to see 2-22
0.342316 ABIN615299 EIA IF IHC (p) WB Rabbit AA 299-318, N-Term Log in to see Polyclonal
0.0008986177 ABIN358092 EIA IHC (p) WB Rabbit Ig pSer196 Log in to see Polyclonal 5
0.0008986177 ABIN392509 IF IHC (p) WB Rabbit Ig AA 183-211, Center Log in to see Polyclonal 3
0.0008986177 ABIN389526 IHC (p) WB Rabbit Ig pSer196 Log in to see Polyclonal 4
0.0008986177 ABIN724160 IF (p) IHC (p) WB Rabbit IgG AA 20-69 Log in to see Polyclonal 4
0.0008986177 ABIN271876 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0008986177 ABIN181322 IHC (p) IP WB Rabbit Log in to see Polyclonal 2
0.0008986177 ABIN127028 IF IHC (p) IP WB Rabbit Asp330, N-Term Log in to see Polyclonal
0.0008986177 ABIN372406 IHC (p) WB Rabbit IgG AA 345-359, AA 366-383 Log in to see Polyclonal
0.0008986177 ABIN1881137 FACS IHC (p) WB Rabbit Ser196 Log in to see Polyclonal 5
0.0008986177 ABIN614358 IHC (p) WB Mouse IgG1 Log in to see 01-02-22
0.0008986177 ABIN683863 IF (p) IHC (p) WB Rabbit IgG pThr125 Log in to see Polyclonal
0.0008986177 ABIN957195 IHC (fro) IHC (p) IP WB Rabbit AA 112-130 Log in to see Polyclonal
0.0008986177 ABIN2888591 IF IHC (p) ELISA WB Rabbit IgG AA 91-140 Log in to see Polyclonal
0.0008986177 ABIN3044458 IHC (p) WB Rabbit IgG AA 183-198, Middle Region Log in to see Polyclonal
0.0008986177 ABIN724175 IF (p) IHC (p) WB Rabbit IgG AA 280-330 Log in to see Polyclonal
0.0008986177 ABIN957196 IHC (fro) IHC (p) IP WB Rabbit AA 188-206 Log in to see Polyclonal

Top referenced anti-Caspase 9 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-Caspase 9 Antibodies

Application / Reactivity Human
ELISA 138 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 67 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 116 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 73 Antibodies
Immunoprecipitation (IP) 68 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 343 Antibodies

General

Antigen Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
Reactivity Human
(413), (119), (116), (19), (13), (9), (6), (5), (4), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(420), (46), (1)
Clonality
Polyclonal
Conjugate This Caspase 9 antibody is un-conjugated
(11), (10), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(390), (161), (128), (77), (68), (68), (45), (34), (13), (13), (8), (6), (2), (2), (2)
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Product Details anti-Caspase 9 Antibody

Target Details Caspase 9 Application Details Handling Images
Specificity This antibody detects endogenous levels of Caspase 9 protein.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity chromatography using epitope-specific immunogen
Purity > 95 % (by SDS-PAGE)

Target Details Caspase 9

Product Details anti-Caspase 9 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. Pr °Caspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, BclXL, cIAP1, cIAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf1/cytochrome c complex, and following cleavage by Apaf1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 intereacts with BIRC7 and has been shown to cleave PARP and vimentin.Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6
Molecular Weight approx. 35 kDa
Gene ID 842
NCBI Accession NP_001220
UniProt P55211
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling Images back to top
Application Notes Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN498253) Immunohistochemistry analyzes of Caspase 9 antibody in paraffin-embedded human...
Western Blotting (WB) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN498253) Western blot analysis of Caspase 9 antibody in extracts from HeLa cells.