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anti-Human Caspase 9 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Caspase 9 Antibody (supplied by: Log in to see )

Antigen
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
  • CASP9
  • AI115399
  • APAF-3
  • AW493809
  • CASP-9
  • Caspase-9
  • ICE-LAP6
  • Mch6
  • Apaf3
  • Casp-9-CTD
  • Casp9_v1
  • Ice-Lap6
  • APAF3
  • MCH6
  • PPP1R56
  • caspase 9
  • caspase 9, apoptosis-related cysteine peptidase
  • caspase-9-like
  • CASP9
  • LOC100101592
  • Casp9
  • LOC100518913
Reactivity
Human
427
152
145
24
15
11
7
6
6
3
2
1
Host
Mouse
423
45
1
Clonality
Monoclonal
Conjugate
This Caspase 9 antibody is un-conjugated
11
10
7
4
4
4
4
4
4
4
4
4
4
4
4
3
3
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
402
160
141
84
74
71
53
30
23
15
13
9
6
2
2
2
Supplier
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Catalog No. ABIN153015
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.929441 ABIN724160 FACS IF (p) IHC (p) WB Rabbit IgG AA 20-69 Log in to see Polyclonal 5
12.929441 ABIN4288194 FACS IHC IHC (fro) IHC (p) IP Rabbit Log in to see Polyclonal 15
12.929441 ABIN498253 IF IHC (p) WB Rabbit Log in to see Polyclonal
12.929441 ABIN498192 IF IHC (p) WB Rabbit Log in to see Polyclonal
12.929441 ABIN615299 EIA IF IHC (p) WB Rabbit AA 299-318, N-Term Log in to see Polyclonal
12.929441 ABIN756922 IF (p) IHC (p) WB Rabbit IgG AA 180-220, pSer196 Log in to see Polyclonal
12.929441 ABIN462358 IHC (p) IP WB Mouse IgG1 Log in to see 2-22
12.929441 ABIN4288203 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
12.929441 ABIN4288196 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN358092 EIA IHC (p) WB Rabbit Ig pSer196 Log in to see Polyclonal 5
1 ABIN389526 IHC (p) WB Rabbit Ig pSer196 Log in to see Polyclonal 4
1 ABIN392509 IF IHC (p) WB Rabbit Ig AA 183-211, Center Log in to see Polyclonal 3
1 ABIN683863 IF (p) IHC (p) WB Rabbit IgG pThr125 Log in to see Polyclonal 1
1 ABIN181322 IHC (p) IP WB Rabbit Log in to see Polyclonal 2
1 ABIN271876 IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN4288190 IHC IHC (fro) IHC (p) IP SimWes WB Rabbit Proform Log in to see Polyclonal 6
1 ABIN724175 IF (p) IHC (p) WB Rabbit IgG AA 280-330 Log in to see Polyclonal 3
1 ABIN1881137 FACS IHC (p) WB Rabbit Ser196 Log in to see Polyclonal 5
1 ABIN127028 IF IHC (p) IP WB Rabbit Asp330, N-Term Log in to see Polyclonal
1 ABIN372406 IHC (p) WB Rabbit IgG AA 345-359, AA 366-383 Log in to see Polyclonal

Top referenced anti-Caspase 9 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-Caspase 9 Antibodies

Application / Reactivity Human
ELISA 139 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 23 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 24 Antibodies
Immunofluorescence (IF) 69 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 53 Antibodies
Immunohistochemistry (IHC) 126 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 82 Antibodies
Immunoprecipitation (IP) 72 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Simple Western (SimWes) 2 Antibodies
Western Blotting (WB) 361 Antibodies

General

Antigen Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
Reactivity Human
(427), (152), (145), (24), (15), (11), (7), (6), (6), (3), (2), (1)
Host Mouse
(423), (45), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Caspase 9 antibody is un-conjugated
(11), (10), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(402), (160), (141), (84), (74), (71), (53), (30), (23), (15), (13), (9), (6), (2), (2), (2)
Pubmed 5 references available
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Product Details anti-Caspase 9 Antibody

Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN153015) Images
Purification Unpurified
Immunogen Recombinant human Caspase 9 pro-domain protein. [UniProt# P55211]
Clone LAP6 96-2-22
Isotype IgG1 kappa

Target Details Caspase 9

Product Details anti-Caspase 9 Antibody Application Details Handling References for anti-Caspase 9 Antibody (ABIN153015) Images back to top
Antigen
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background Gene Symbol: CASP9
Gene ID 842
UniProt P55211
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling References for anti-Caspase 9 Antibody (ABIN153015) Images back to top
Application Notes Western Blot 1:500-1:1000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:50-1:250, Immunohistochemistry-Paraffin 1:50-1:250This Caspase 9 (LAP6 96-2-22) antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where bands at ~46 kDa-48 kDa and 35 kDa are seen representing the pro and active forms of Caspase 9, respectively. In ICC/IF cytoplasmic staining can be seen in HeLa cells. Paraffin data from customer review.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western protocol specific for Caspase 9 Antibody Procedure Guide for NB 500-209 Monoclonal anti-Caspase 9

Western Protocol

1) Run a 10-*15 % SDS polyacrylamide gel, loading ~20 µg of cell extract per lane.
2) Transfer the proteins to a membrane.
3) Block the membrane in PT-T20 [20 mM Tris-HCl, pH 7.4 / 150 mM NaCl/0.5 % Tween 20] + 5 % NFDM [non-fat dry milk], for 3 hour at room temperature (RT), shaking gently.
4) Rinse the membrane twice with PT-T20.
5) Incubate the membrane in anti-Caspase 9 [NB 500-209], diluted 1:500-1:1,000 in PT-T20 + 5 % NFDM, for 60 minutes at RT.
6) Wash the membrane for 15 minutes, 3 times, in PT-T20 at RT.
7) Incubate the membrane in anti-mouse conjugated to HRP (secondary antibody), diluted in PT-T20 + 5 % NFDM for 60 minutes at RT.
8) Wash the membrane for 15 minutes, 3 times, in PT-T20 at RT.
9) Develop membrane in chemiluminescent reagents, as instructed by kit-vendor.

*Positive control(s): Human kidney 293 cells

To Activate the Cell Extracts:
Bring the extract to a final concentration of 5 mM dATP.
Incubate the extracts at 37?C for 15-30 minutes.Immunocytochemistry/Immunofluorescence Protocol for Caspase 9 Antibody Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details References for anti-Caspase 9 Antibody (ABIN153015) Images back to top
Format Liquid
Concentration 30.7 mg/mL
Buffer Ascites
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-Caspase 9 Antibody (ABIN153015)

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling Images back to top
Product cited in:

Hsuan, Chyau, Hung, Chen, Chou: "The induction of apoptosis and autophagy by Wasabia japonica extract in colon cancer." in: European journal of nutrition, Vol. 55, Issue 2, pp. 491-503, 2016 (Sample species: Human). Further details: Western Blotting

Kim, Lee, Park, Chung, Im, Noh, Han, Moon, Jung, Ryu: "Expression of SIRT1 and apoptosis-related proteins is predictive for lymph node metastasis and disease-free survival in luminal A breast cancer." in: Virchows Archiv : an international journal of pathology, Vol. 467, Issue 5, pp. 563-70, 2015 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Martin, Fearnhead: "Apocytochrome c blocks caspase-9 activation and Bax-induced apoptosis." in: The Journal of biological chemistry, Vol. 277, Issue 52, pp. 50834-41, 2002 (Sample species: Human). Further details: Western Blotting

Background publications

Kirsch, Doseff, Chau, Lim, de Souza-Pinto, Hansford, Kastan, Lazebnik, Hardwick: "Caspase-3-dependent cleavage of Bcl-2 promotes release of cytochrome c." in: The Journal of biological chemistry, Vol. 274, Issue 30, pp. 21155-61, 1999

Fearnhead, Rodriguez, Govek, Guo, Kobayashi, Hannon, Lazebnik: "Oncogene-dependent apoptosis is mediated by caspase-9." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 23, pp. 13664-9, 1998

Images

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN153015) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN153015) The pro and active forms of Caspase 9 detected in active 293 cell lysate (lane 2). La...
Western Blotting (WB) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN153015) Western Blot: Caspase 9 Antibody (LAP6 96-2-22) [ABIN153015] - Western blot analysis ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN153015) Immunohistochemistry-Paraffin: Caspase 9 Antibody (LAP6 96-2-22) [ABIN153015] - IHC a...
Immunofluorescence (IF) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN153015) Immunocytochemistry/Immunofluorescence: Caspase 9 Antibody (LAP6 96-2-22) [ABIN153015...