You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human Caspase 9 antibody for Immunoprecipitation

Recommended Caspase 9 Antibody (supplied by: Log in to see )

Antigen
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
  • AI115399
  • APAF-3
  • APAF3
  • Apaf3
  • AW493809
  • CASP-9
  • Casp-9-CTD
  • CASP9
  • Casp9_v1
  • Caspase-9
  • Ice-Lap6
  • ICE-LAP6
  • MCH6
  • Mch6
  • PPP1R56
Epitope
Asp330, N-Term, active
35
22
20
19
18
16
13
13
13
13
12
11
11
8
8
7
7
6
6
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
413
118
115
19
13
9
5
4
4
2
2
2
1
1
1
1
Host
Rabbit
419
47
1
Clonality
Polyclonal
Conjugate
This Caspase 9 antibody is un-conjugated
11
10
7
4
4
4
4
4
4
4
4
4
3
3
3
Application
Immunoprecipitation (IP), Western Blotting (WB)
390
161
129
79
70
67
45
35
13
13
8
6
2
2
2
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN222956
$ 430.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089556724 ABIN181322 IHC (p) IP WB Rabbit Log in to see Polyclonal 2
0.00089556724 ABIN127028 IF IHC (p) IP WB Rabbit Asp330, N-Term Log in to see Polyclonal
0.00089556724 ABIN957195 IHC (fro) IHC (p) IP WB Rabbit AA 112-130 Log in to see Polyclonal
0.00089556724 ABIN957196 IHC (fro) IHC (p) IP WB Rabbit AA 188-206 Log in to see Polyclonal
0.00089556724 ABIN957194 IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
0.00089556724 ABIN462358 IHC (p) IP WB Mouse IgG1 Log in to see 2-22
0.00089556724 ABIN2156145 ELISA IP WB Rabbit AA 41-56 Log in to see Polyclonal
0.00089556724 ABIN957193 IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal
0.00089556724 ABIN2469976 IP IHC WB Mouse IgG1 Log in to see 01-02-22
0.00089556724 ABIN957197 IHC (fro) IHC (p) IP WB Rabbit AA 188-206 Log in to see Polyclonal
0.00089556724 ABIN1821833 IP WB Rabbit IgG Asp330 Log in to see Polyclonal
0.00089556724 ABIN2856878 IP WB Rabbit Center Log in to see Polyclonal
0.00089556724 ABIN219279 IP WB Rabbit IgG N-Term Log in to see Polyclonal
0.00089556724 ABIN219278 IP ELISA WB Rabbit IgG Asp315 Log in to see Polyclonal
0.00089556724 ABIN387074 IHC (p) IP WB Biotin Rabbit IgG AA 1-134 Log in to see Polyclonal
0.00089556724 ABIN387070 IHC (p) IP WB Rabbit IgG AA 1-134 Log in to see Polyclonal
0.00089556724 ABIN1723262 IHC (p) IP WB Mouse IgG1 Log in to see 2-22
0.00089556724 ABIN219275 IP WB Rabbit Log in to see Polyclonal
0.00089556724 ABIN198479 ICC IP WB Rabbit Log in to see Polyclonal
0.00089556724 ABIN2736407 ICC FACS IP IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-Caspase 9 antibody for Immunoprecipitation

Similar anti-Caspase 9 Antibodies

Application / Reactivity Human
ELISA 138 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 67 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 117 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 74 Antibodies
Immunoprecipitation (IP) 68 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 342 Antibodies

General

Antigen Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
Epitope Asp330, N-Term, active
(35), (22), (20), (19), (18), (16), (13), (13), (13), (13), (12), (11), (11), (8), (8), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(413), (118), (115), (19), (13), (9), (5), (4), (4), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(419), (47), (1)
Clonality
Polyclonal
Conjugate This Caspase 9 antibody is un-conjugated
(11), (10), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3)
Application Immunoprecipitation (IP), Western Blotting (WB)
(390), (161), (129), (79), (70), (67), (45), (35), (13), (13), (8), (6), (2), (2), (2)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-Caspase 9 Antibody

Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN222956) Images
Specificity The anti-active caspase-9 antibody recognizes only the cleaved caspase-9 (37 kDa).
It does not recognize full-length caspase-9 or any other caspases.
Purification Affinity purified
Immunogen Synthetic peptide mapping to the N-terminus adjacent to Asp330 of human caspase-9
Isotype IgG

Target Details Caspase 9

Product Details anti-Caspase 9 Antibody Application Details Handling References for anti-Caspase 9 Antibody (ABIN222956) Images back to top
Antigen
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase-9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase-9 (47 kDa) and Apaf-1. The complex processes pro-caspase-9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase-9 further processes other caspases including caspase-3 and caspase-6, to initiate a caspase cascade leading to apoptosis. The affinity purified antibody recognizing the active forms of caspase-9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Synonyms: CASP-9, EC 3.4.22.62, ICE-like apoptotic protease 6,ICE-LAP6,Apoptotic protease Mch-6,Apoptotic protease-activating factor 3, Apaf-3
Gene ID 12371
UniProt P55211
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling References for anti-Caspase 9 Antibody (ABIN222956) Images back to top
Application Notes Western blotting (0.5-4 μg/mL) and Immunoprecipitation (10-20 μg/mL).
However, the optimal concentrations should be determined individually.
Comment

Country of Animal Origin: China

Restrictions For Research Use only

Handling

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details References for anti-Caspase 9 Antibody (ABIN222956) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50 % glycerol, 0.5 % BSA, and 0.02 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-Caspase 9 Antibody (ABIN222956)

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling Images back to top
Product cited in:

Kasloff, Pizzuto, Silic-Benussi, Pavone, Ciminale, Capua: "Oncolytic activity of avian influenza virus in human pancreatic ductal adenocarcinoma cell lines." in: Journal of virology, Vol. 88, Issue 16, pp. 9321-34, 2014

Schwartz, Barker, Kaufman, Fayram, McCracken, Allen: "Francisella tularensis inhibits the intrinsic and extrinsic pathways to delay constitutive apoptosis and prolong human neutrophil lifespan." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 7, pp. 3351-63, 2012

Scarabelli, Pasini, Ferrari, Ferrari, Stephanou, Lawrence, Townsend, Chen-Scarabelli, Gitti, Saravolatz, Latchman, Knight, Gardin: "Warm blood cardioplegic arrest induces mitochondrial-mediated cardiomyocyte apoptosis associated with increased urocortin expression in viable cells." in: The Journal of thoracic and cardiovascular surgery, Vol. 128, Issue 3, pp. 364-71, 2004

Scarabelli, Stephanou, Pasini, Comini, Raddino, Knight, Latchman: "Different signaling pathways induce apoptosis in endothelial cells and cardiac myocytes during ischemia/reperfusion injury." in: Circulation research, Vol. 90, Issue 6, pp. 745-8, 2002

Images

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN222956) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (Asp330) (ABIN222956) Western blot analysis with Jurkat cell lysate. Lane Untreated Lane Etoposide treated.