anti-Human Caspase 9 antibody for Western Blotting

Recommended Caspase 9 Antibody (supplied by: Log in to see )

Antigen
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
  • CASP9
  • AI115399
  • APAF-3
  • AW493809
  • CASP-9
  • Caspase-9
  • ICE-LAP6
  • Mch6
  • Apaf3
  • Casp-9-CTD
  • Casp9_v1
  • Ice-Lap6
  • APAF3
  • MCH6
  • PPP1R56
  • caspase 9
  • caspase 9, apoptosis-related cysteine peptidase
  • caspase-9-like
  • CASP9
  • LOC100101592
  • Casp9
  • LOC100518913
Reactivity
Cow (Bovine), Hamster, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
432
149
139
23
12
9
6
6
4
2
1
1
Host
Rabbit
426
47
1
Clonality
Polyclonal
Conjugate
This Caspase 9 antibody is un-conjugated
11
10
7
4
4
4
4
4
4
4
4
4
4
4
4
3
3
Application
Western Blotting (WB)
408
150
137
92
75
74
53
33
23
15
13
10
6
2
2
2
1
Options
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Catalog No. ABIN222874
$ 265.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9060946 ABIN783314 WB Rabbit AA 301-305 Log in to see Polyclonal 3
2.9060946 ABIN4288190 IHC IHC (fro) IHC (p) IP SimWes WB Rabbit Proform Log in to see Polyclonal 6
2.9060946 ABIN724160 FACS IF (p) IHC (p) WB Rabbit IgG AA 20-69 Log in to see Polyclonal 6
2.9060946 ABIN222950 WB Rabbit IgG Log in to see Polyclonal
2.9060946 ABIN3043748 WB Rabbit IgG AA 3-228 Log in to see Polyclonal 14
2.9060946 ABIN389526 IHC (p) WB Rabbit Ig pSer196 Log in to see Polyclonal 4
2.9060946 ABIN392509 IF IHC (p) WB Rabbit Ig AA 183-211, Center Log in to see Polyclonal 3
2.9060946 ABIN222956 IP WB Rabbit IgG Asp330, N-Term, active Log in to see Polyclonal 4
2.9060946 ABIN153015 ICC IF IHC IHC (p) WB Mouse IgG1 kappa Log in to see LAP6 96-2-22 5
2.9060946 ABIN393211 IF WB Mouse IgG1 kappa AA 1-417 Log in to see 3B8-4G2 5
2.9060946 ABIN967338 WB Mouse IgG1, kappa AA 1-134, N-Term Log in to see 2 6
2.9060946 ABIN967339 WB Mouse IgG1, kappa AA 1-134, N-Term Log in to see 2 6
2.9060946 ABIN967543 WB Mouse IgG1, kappa AA 364-405 Log in to see B40 4
2.9060946 ABIN1881137 FACS IHC (p) WB Rabbit Ser196 Log in to see Polyclonal 5
2.9060946 ABIN3044458 IHC (p) WB Rabbit IgG AA 183-198, Middle Region Log in to see Polyclonal 15
2.9060946 ABIN2477925 ELISA WB Rabbit IgG AA 41-56 Log in to see Polyclonal 2
2.9060946 ABIN4288198 IHC IHC (p) IP WB Rabbit Proform Log in to see Polyclonal 2
2.9060946 ABIN1536088 IHC ELISA WB Rabbit IgG Cleaved-Asp330 Log in to see Polyclonal
2.9060946 ABIN1536087 IHC ELISA WB Rabbit IgG Cleaved-Asp315 Log in to see Polyclonal
2.9060946 ABIN1531532 IF ELISA WB Rabbit IgG pTyr153 Log in to see Polyclonal

General

Antigen Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
Reactivity Cow (Bovine), Hamster, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(432), (149), (139), (23), (12), (9), (6), (6), (4), (2), (1), (1)
Host Rabbit
(426), (47), (1)
Clonality
Polyclonal
Conjugate This Caspase 9 antibody is un-conjugated
(11), (10), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3)
Application Western Blotting (WB)
(408), (150), (137), (92), (75), (74), (53), (33), (23), (15), (13), (10), (6), (2), (2), (2), (1)
Pubmed 3 references available
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Product Details anti-Caspase 9 Antibody

Target Details Caspase 9 Application Details Handling ProductDetails: References for anti-Caspase 9 antibody (ABIN222874) Images
Blocking Peptide Blocking peptide for this product available: ABIN412659
Specificity The antibody detects proform (~46 kDa), intermediate (~33-36 kDa), and the fully cleaved (~20-25 kDa) caspase-9.
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 290 of human caspase-9
Isotype IgG

Target Details Caspase 9

Product Details anti-Caspase 9 Antibody Application Details Handling ProductDetails: References for anti-Caspase 9 antibody (ABIN222874) Images back to top
Antigen
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background Caspase-9 is one of the most important caspases among the caspase family members. Caspase-9 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis. The processed caspase-9 consists of large (32-35 kD) and small (10 kD) subunits which associate to form an active enzyme. Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis.
Synonyms: CASP-9 , EC 3.4.22.62 , ICE-like apoptotic protease 6 ,ICE-LAP6 ,Apoptotic protease Mch-6 ,Apoptotic protease-activating factor 3 , Apaf-3
Gene ID 842
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling ProductDetails: References for anti-Caspase 9 antibody (ABIN222874) Images back to top
Application Notes Western blotting (0.5-4 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: China

Restrictions For Research Use only

Handling

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details ProductDetails: References for anti-Caspase 9 antibody (ABIN222874) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-caspase-9 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

ProductDetails: References for anti-Caspase 9 antibody (ABIN222874)

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling Images back to top
Product cited in:

Woods, Burns, Bradford, Ross, Kosyk, Swenberg, Cunningham, Rusyn: "WY-14,643 induced cell proliferation and oxidative stress in mouse liver are independent of NADPH oxidase." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 98, Issue 2, pp. 366-74, 2007

Takebe, Cheng, Fandy, Srivastava, Wu, Shankar, Bauer, Shaughnessy, Tricot: "IMP dehydrogenase inhibitor mycophenolate mofetil induces caspase-dependent apoptosis and cell cycle inhibition in multiple myeloma cells." in: Molecular cancer therapeutics, Vol. 5, Issue 2, pp. 457-66, 2006

Siu, Alway: "Mitochondria-associated apoptotic signalling in denervated rat skeletal muscle." in: The Journal of physiology, Vol. 565, Issue Pt 1, pp. 309-23, 2005

Images

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling ProductDetails: References for anti-Caspase 9 antibody (ABIN222874) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) antibody (ABIN222874) Western blot analysis of Caspase 9with rat kidney tissue lysate.