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anti-Human Caspase 9 antibody for Western Blotting

Recommended Caspase 9 Antibody (supplied by: Log in to see )

Antigen
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
  • AI115399
  • APAF-3
  • APAF3
  • Apaf3
  • AW493809
  • CASP-9
  • Casp-9-CTD
  • CASP9
  • Casp9_v1
  • Caspase-9
  • Ice-Lap6
  • ICE-LAP6
  • MCH6
  • Mch6
  • PPP1R56
Epitope
AA 301-305
35
22
21
19
18
16
13
13
13
13
12
11
11
9
8
7
7
7
6
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
413
118
115
19
13
9
5
4
4
2
2
2
1
1
1
1
Host
Rabbit
419
47
1
Clonality
Polyclonal
Conjugate
This Caspase 9 antibody is un-conjugated
11
10
7
4
4
4
4
4
4
4
4
4
3
3
3
Application
Western Blotting (WB)
390
161
129
79
70
68
45
35
13
13
8
6
2
2
2
Supplier
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Catalog No. ABIN783314
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN222956 IP WB Rabbit IgG Asp330, N-Term, active Log in to see Polyclonal 4
0.13341247 ABIN392509 IF IHC (p) WB Rabbit Ig AA 183-211, Center Log in to see Polyclonal 3
0.13341247 ABIN389526 IHC (p) WB Rabbit Ig pSer196 Log in to see Polyclonal 4
0.13341247 ABIN438247 ICC IF IHC IHC (p) WB Mouse IgG1 kappa Log in to see LAP6 96-2-22 5
0.13341247 ABIN264409 WB Mouse IgG Log in to see LAP6 96-2-22 3
0.13341247 ABIN724160 IF (p) IHC (p) WB Rabbit IgG AA 20-69 Log in to see Polyclonal 4
0.13341247 ABIN499577 EIA WB Rabbit IgG Intermediate Domain 1 Log in to see Polyclonal 4
0.13341247 ABIN499579 EIA IF WB Rabbit IgG Intermediate Domain 2 Log in to see Polyclonal 1
0.13341247 ABIN393211 IF WB Mouse IgG1 kappa AA 1-417 Log in to see 3B8-4G2 5
0.13341247 ABIN967338 WB Mouse IgG1, kappa AA 1-134, N-Term Log in to see 2 6
0.13341247 ABIN967339 WB Mouse IgG1, kappa AA 1-134, N-Term Log in to see 2 6
0.13341247 ABIN967543 WB Mouse IgG1, kappa AA 364-405 Log in to see B40 4
0.13341247 ABIN1881137 FACS IHC (p) WB Rabbit Ser196 Log in to see Polyclonal 5
0.13341247 ABIN1531533 IHC ELISA WB Rabbit IgG pSer144 Log in to see Polyclonal
0.13341247 ABIN1531532 IF ELISA WB Rabbit IgG pTyr153 Log in to see Polyclonal
0.13341247 ABIN1531534 ELISA WB Rabbit IgG pSer196 Log in to see Polyclonal
0.13341247 ABIN1531216 IHC ELISA WB Rabbit IgG pThr125 Log in to see Polyclonal
0.13341247 ABIN1536087 IHC ELISA WB Rabbit IgG Cleaved-Asp315 Log in to see Polyclonal
0.13341247 ABIN1536088 IHC ELISA WB Rabbit IgG Cleaved-Asp330 Log in to see Polyclonal
0.13341247 ABIN222950 WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-Caspase 9 antibody for Western Blotting

Similar anti-Caspase 9 Antibodies

Application / Reactivity Human
ELISA 138 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 67 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 117 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 74 Antibodies
Immunoprecipitation (IP) 68 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 342 Antibodies

General

Antigen Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
Epitope AA 301-305
(35), (22), (21), (19), (18), (16), (13), (13), (13), (13), (12), (11), (11), (9), (8), (7), (7), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(413), (118), (115), (19), (13), (9), (5), (4), (4), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(419), (47), (1)
Clonality
Polyclonal
Conjugate This Caspase 9 antibody is un-conjugated
(11), (10), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3)
Application Western Blotting (WB)
(390), (161), (129), (79), (70), (68), (45), (35), (13), (13), (8), (6), (2), (2), (2)
Pubmed 3 references available
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Product Details anti-Caspase 9 Antibody

Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN783314) Images
Sequence T-S-P-E-D
Specificity This antibody detects endogenous level of total Caspase 9 protein.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Affinity-chromatography using epitope-specific peptide
Immunogen Peptide sequence around aa.301~305 derived from Caspase 9

Target Details Caspase 9

Product Details anti-Caspase 9 Antibody Application Details Handling References for anti-Caspase 9 Antibody (ABIN783314) Images back to top
Antigen
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6
Molecular Weight 47/37/35 kDa
Gene ID 842
NCBI Accession NP_001220
UniProt P55211
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling References for anti-Caspase 9 Antibody (ABIN783314) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details References for anti-Caspase 9 Antibody (ABIN783314) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.

References for anti-Caspase 9 Antibody (ABIN783314)

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling Images back to top
Background publications

Zou, Li, Liu, Wang: "An APAF-1.cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9." in: The Journal of biological chemistry, Vol. 274, Issue 17, pp. 11549-56, 1999

Deveraux, Roy, Stennicke, Van Arsdale, Zhou, Srinivasula, Alnemri, Salvesen, Reed: "IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases." in: The EMBO journal, Vol. 17, Issue 8, pp. 2215-23, 1998

Kuroda, Meller: "Transient Xist-ence." in: Cell, Vol. 91, Issue 1, pp. 9-11, 1997

Images

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN783314) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (AA 301-305) (ABIN783314) Western blot analysis of extracts from 293 and Hela cells using Caspase 9.