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anti-Monkey Caspase 9 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Caspase 9 Antibody (supplied by: Log in to see )

Antigen
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
  • AI115399
  • APAF-3
  • APAF3
  • Apaf3
  • AW493809
  • CASP-9
  • Casp-9-CTD
  • CASP9
  • Casp9_v1
  • Caspase-9
  • Ice-Lap6
  • ICE-LAP6
  • MCH6
  • Mch6
  • PPP1R56
Reactivity
Human, Monkey
413
119
116
19
12
9
6
5
4
3
3
2
1
1
1
1
Host
Mouse
421
45
1
Clonality
Monoclonal
Conjugate
This Caspase 9 antibody is un-conjugated
11
10
7
4
4
4
4
4
4
4
4
4
3
3
3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
390
161
128
77
69
68
45
34
13
13
8
6
2
2
2
Supplier
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Catalog No. ABIN614358
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089690165 ABIN1723262 IHC (p) IP WB Mouse IgG1 Log in to see 2-22

Similar anti-Caspase 9 Antibodies

Application / Reactivity Monkey Human
ELISA 2 Antibodies 138 Antibodies
Immunochromatography (IC) 1 Antibodies 8 Antibodies
Immunofluorescence (IF) 2 Antibodies 67 Antibodies
Immunohistochemistry (IHC) 4 Antibodies 116 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies 73 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 11 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 11 Antibodies

General

Antigen Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
Reactivity Human, Monkey
(413), (119), (116), (19), (12), (9), (6), (5), (4), (3), (3), (2), (1), (1), (1), (1)
Host Mouse
(421), (45), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Caspase 9 antibody is un-conjugated
(11), (10), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(390), (161), (128), (77), (69), (68), (45), (34), (13), (13), (8), (6), (2), (2), (2)
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Product Details anti-Caspase 9 Antibody

Target Details Caspase 9 Application Details Handling Images
Specificity Detects an ~46-50 kDa protein, corresponding to the apparent molecular mass of the Caspase-9 on SDS-PAGE Immunoblots, in samples from Human and Monkey origins. This antibody recognizes the Pro-Caspase-9 Human recombinant protein as well as Pro-Caspase -9 from Human and Monkey cell lysates.
Cross-Reactivity (Details) Species reactivity (expected):Monkey.
Species reactivity (tested):Human.
Purification Protein G Chromatography
Immunogen Recombinant prodomain of Human Caspase 9.
Clone 01-02-22
Isotype IgG1

Target Details Caspase 9

Product Details anti-Caspase 9 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis, initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. Pr °Caspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, BclXL, cIAP1, cIAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf1/cytochrome c complex, and following cleavage by Apaf1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 intereacts with BIRC7 and has been shown to cleave PARP and vimentin.Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6
Gene ID 842
NCBI Accession NP_001220
UniProt P55211
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Images back to top
Buffer PBS with 0.1 mM PMSF and 50 % Glycerol
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN614358) anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) antibody
Immunohistochemistry (IHC) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN614358) anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN614358) anti-Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) antibody (Image 3)