|Antigen||Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies|
|Epitope||Intermediate Domain 1 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Caspase 9 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-Caspase 9 AntibodyTarget Details Caspase 9 Application Details Handling Images
|Specificity||Caspase-9 antibody was raised against a peptide corresponding to amino acids 41 to 56 of human caspase-9. It has no cross response to other members in caspase family.|
|Purification||Affinity chromatography purified via peptide column|
|Immunogen||Human Caspase-9 (Intermediate Domain 1) Peptide|
Target Details Caspase 9Product Details anti-Caspase 9 Antibody Application Details Handling Images back to top
|Alternative Name||Caspase-9 (CASP9 Antibody Abstract)|
|Background||Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3 (1-3). Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation (3). Caspase-9 is also activated by granzyme B and CPP32 (1,2). Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFalpha, TRAIL, anti-CD-95, FADD, and TRADD (4). Caspase-9 is expressed in a variety of human tissues (1,2).Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6|
|Research Area||Apoptosis/Necrosis, Cancer|
|Pathways||MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling Images back to top
ELISA. Western Blot: Caspase-9 antibody can be used for detection of caspase-9 at 1/1000 to1/2000 dilution. A 46 kDa band should be detected.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Images back to top
|Buffer||PBS containing 0.02 % sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|