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anti-Mouse (Murine) Caspase 9 antibody for Enzyme Immunoassay

Recommended Caspase 9 Antibody (supplied by: Log in to see )

Antigen
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
  • AI115399
  • APAF-3
  • APAF3
  • Apaf3
  • AW493809
  • CASP-9
  • Casp-9-CTD
  • CASP9
  • Casp9_v1
  • Caspase-9
  • Ice-Lap6
  • ICE-LAP6
  • MCH6
  • Mch6
  • PPP1R56
Epitope
Intermediate Domain 1
35
22
21
19
18
16
13
13
13
13
12
11
11
9
8
7
7
7
6
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
412
117
114
19
13
9
5
4
4
2
2
2
1
1
1
1
Host
Rabbit
419
46
1
Clonality
Polyclonal
Conjugate
This Caspase 9 antibody is un-conjugated
11
10
7
4
4
4
4
4
4
4
4
4
3
3
3
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
389
161
128
78
69
68
45
34
13
13
8
5
2
2
2
Supplier
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Catalog No. ABIN499577
$ 401.50
Plus shipping costs $45.00

Top referenced anti-Caspase 9 antibody for Enzyme Immunoassay

Similar anti-Caspase 9 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 342 Antibodies 99 Antibodies 97 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Immunoprecipitation (IP) 68 Antibodies 14 Antibodies 15 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 73 Antibodies 21 Antibodies 19 Antibodies
Immunohistochemistry (IHC) 116 Antibodies 49 Antibodies 45 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 11 Antibodies 5 Antibodies 5 Antibodies

General

Antigen Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
Epitope Intermediate Domain 1
(35), (22), (21), (19), (18), (16), (13), (13), (13), (13), (12), (11), (11), (9), (8), (7), (7), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(412), (117), (114), (19), (13), (9), (5), (4), (4), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(419), (46), (1)
Clonality
Polyclonal
Conjugate This Caspase 9 antibody is un-conjugated
(11), (10), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(389), (161), (128), (78), (69), (68), (45), (34), (13), (13), (8), (5), (2), (2), (2)
Pubmed 4 references available
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Product Details anti-Caspase 9 Antibody

Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN499577) Images
Specificity Caspase-9 antibody was raised against a peptide corresponding to amino acids 41 to 56 of human caspase-9. It has no cross response to other members in caspase family.
Purification Affinity chromatography purified via peptide column
Immunogen Human Caspase-9 (Intermediate Domain 1) Peptide
Isotype IgG

Target Details Caspase 9

Product Details anti-Caspase 9 Antibody Application Details Handling References for anti-Caspase 9 Antibody (ABIN499577) Images back to top
Antigen
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3 (1-3). Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation (3). Caspase-9 is also activated by granzyme B and CPP32 (1,2). Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFalpha, TRAIL, anti-CD-95, FADD, and TRADD (4). Caspase-9 is expressed in a variety of human tissues (1,2).Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6
Gene ID 842
UniProt P55211
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling References for anti-Caspase 9 Antibody (ABIN499577) Images back to top
Application Notes ELISA. Western Blot: Caspase-9 antibody can be used for detection of caspase-9 at 1/1000 to1/2000 dilution. A 46 kDa band should be detected.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details References for anti-Caspase 9 Antibody (ABIN499577) Images back to top
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-Caspase 9 Antibody (ABIN499577)

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling Images back to top
Background publications

Pan, ORourke, Dixit: "Caspase-9, Bcl-XL, and Apaf-1 form a ternary complex." in: The Journal of biological chemistry, Vol. 273, Issue 10, pp. 5841-5, 1998

Li, Nijhawan, Budihardjo, Srinivasula, Ahmad, Alnemri, Wang: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997

Duan, Orth, Chinnaiyan, Poirier, Froelich, He, Dixit: "ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B." in: The Journal of biological chemistry, Vol. 271, Issue 28, pp. 16720-4, 1996

Srinivasula, Fernandes-Alnemri, Zangrilli, Robertson, Armstrong, Wang, Trapani, Tomaselli, Litwack, Alnemri: "The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32." in: The Journal of biological chemistry, Vol. 271, Issue 43, pp. 27099-106, 1996

Images

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN499577) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (Intermediate Domain 1) (ABIN499577) Western blot analysis of caspase-9 in HeLa whole cell lysate with AP30205PU-N caspase...