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anti-Mouse (Murine) Caspase 9 antibody for Immunoprecipitation

Recommended Caspase 9 Antibody (supplied by: Log in to see )

Antigen
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
  • AI115399
  • APAF-3
  • APAF3
  • Apaf3
  • AW493809
  • CASP-9
  • Casp-9-CTD
  • CASP9
  • Casp9_v1
  • Caspase-9
  • Ice-Lap6
  • ICE-LAP6
  • MCH6
  • Mch6
  • PPP1R56
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
397
109
106
15
13
9
6
4
3
3
1
1
1
1
Host
Rabbit
404
45
1
Clonality
Polyclonal
Conjugate
This Caspase 9 antibody is un-conjugated
11
10
7
4
4
4
4
4
4
4
4
4
3
3
3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
375
159
114
66
63
56
45
31
13
9
6
6
2
2
Supplier
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Catalog No. ABIN181322
$ 554.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009004611 ABIN2156145 ELISA IP WB Rabbit AA 41-56 Log in to see Polyclonal
0.0009004611 ABIN2736407 ICC FACS IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0009004611 ABIN1584163 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0009004611 ABIN2852722 IC IF IP WB Mouse Log in to see
0.0009004611 ABIN2561590 FACS IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0009004611 ABIN3181147 IP WB Mouse Log in to see
0.0009004611 ABIN3067821 ICC IF IP WB Mouse Log in to see
0.0009004611 ABIN3059288 ICC FACS IF IP IHC WB Rabbit Log in to see Polyclonal
0.0009004611 ABIN3059723 ICC FACS IF IP IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-Caspase 9 antibody for Immunoprecipitation

Similar anti-Caspase 9 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Sheep (Ovine)
Western Blotting (WB) 329 Antibodies 93 Antibodies 91 Antibodies 5 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Immunoprecipitation (IP) 57 Antibodies 10 Antibodies 1 Antibodies 11 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 60 Antibodies 14 Antibodies 12 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 103 Antibodies 42 Antibodies

General

Antigen Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
(397), (109), (106), (15), (13), (9), (6), (4), (3), (3), (1), (1), (1), (1)
Host Rabbit
(404), (45), (1)
Clonality
Polyclonal
Conjugate This Caspase 9 antibody is un-conjugated
(11), (10), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(375), (159), (114), (66), (63), (56), (45), (31), (13), (9), (6), (6), (2), (2)
Pubmed 2 references available
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Product Details anti-Caspase 9 Antibody

Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN181322) Images
Sequence 1-134
Specificity This antibody reacts with Caspase 9.
Cellular Localization: Cytoplasmic.
Purification Protein A Affinity Chromatography
Immunogen Recombinant protein of human Caspase 9

Target Details Caspase 9

Product Details anti-Caspase 9 Antibody Application Details Handling References for anti-Caspase 9 Antibody (ABIN181322) Images back to top
Antigen
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background Activation of procaspase-9 by Apaf-1 in the cytochrome c/ dATP-dependent pathway requires proteolytic cleavage to generate the mature caspase molecule. Deletion of the Apaf-1 WD-40 repeats makes Apaf-1 constitutively active and capable of processing procaspase-9 independent of cytochrome c and dATP. Apaf-1-mediated processing of procaspase-9 occurs at Asp-315 by an intrinsic autocatalytic activity of procaspase-9 itself. Apaf-1 can form oligomers and may facilitate procaspase-9 autoactivation by oligomerizing its precursor molecules. Once activated, Caspase 9 can initiate a caspase cascade involving the downstream executioners Caspase 3, 6, and 7.Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6
Gene ID 842
NCBI Accession NP_001220
UniProt P55211
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling References for anti-Caspase 9 Antibody (ABIN181322) Images back to top
Application Notes Western Blotting (2.5-5 μg/mL for 2hrs at RT). Immunoprecipitation (10g/mg protein lysate, Denatured verified, Use Protein A). Immunohistology (Formalin/paraffin, 5-10g/mL for 30 min at RT. Staining offormalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for10-20 min followed by cooling at RT for 20 min. Recommended positive control: Jurkat Cells, Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details References for anti-Caspase 9 Antibody (ABIN181322) Images back to top
Concentration 1.0 mg/mL
Buffer 10 mM PBS pH 7.4 with 0.09 % Sodium Azide as preservative and 0.2 % BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-Caspase 9 Antibody (ABIN181322)

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling Images back to top
Background publications

Hakem, Hakem, Duncan, Henderson, Woo, Soengas, Elia, de la Pompa, Kagi, Khoo, Potter, Yoshida, Kaufman, Lowe, Penninger, Mak: "Differential requirement for caspase 9 in apoptotic pathways in vivo." in: Cell, Vol. 94, Issue 3, pp. 339-52, 1998

Li, Nijhawan, Budihardjo, Srinivasula, Ahmad, Alnemri, Wang: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997