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anti-Mouse (Murine) Caspase 9 antibody for Western Blotting

Recommended Caspase 9 Antibody (supplied by: Log in to see )

Antigen
Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
  • AI115399
  • APAF-3
  • APAF3
  • Apaf3
  • AW493809
  • CASP-9
  • Casp-9-CTD
  • CASP9
  • Casp9_v1
  • Caspase-9
  • Ice-Lap6
  • ICE-LAP6
  • MCH6
  • Mch6
  • PPP1R56
Reactivity
Mouse (Murine), Rat (Rattus)
421
121
116
21
13
5
4
4
3
2
2
2
1
1
1
1
Host
Rabbit
419
48
1
Clonality
Polyclonal
Conjugate
This Caspase 9 antibody is un-conjugated
11
10
8
7
4
4
4
4
4
4
4
4
4
3
3
Application
Western Blotting (WB)
385
161
131
79
72
67
49
36
20
13
13
6
2
2
2
Supplier
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Catalog No. ABIN223131
$ 283.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.124960825 ABIN392509 IF IHC (p) WB Rabbit Ig AA 183-211, Center Log in to see Polyclonal 3
0.124960825 ABIN499577 EIA WB Rabbit IgG Intermediate Domain 1 Log in to see Polyclonal 4
0.124960825 ABIN724160 FACS IF (p) IHC (p) WB Rabbit IgG AA 20-69 Log in to see Polyclonal 5
0.124960825 ABIN1531216 IHC ELISA WB Rabbit IgG pThr125 Log in to see Polyclonal
0.124960825 ABIN222950 WB Rabbit IgG Log in to see Polyclonal
0.124960825 ABIN1532234 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.124960825 ABIN1536086 IHC ELISA WB Rabbit IgG Cleaved-Asp353 Log in to see Polyclonal
0.124960825 ABIN2705696 IC IF IHC WB Rabbit Center Log in to see Polyclonal
0.124960825 ABIN1871475 WB Mouse IgG Log in to see
0.124960825 ABIN4288190 IHC IHC (fro) IHC (p) IP SimWes WB Rabbit Proform Log in to see Polyclonal 6
0.124960825 ABIN1871476 IF WB Rabbit IgG Log in to see Polyclonal
0.124960825 ABIN2477925 ELISA WB Rabbit IgG AA 41-56 Log in to see Polyclonal 2
0.124960825 ABIN2156145 ELISA IP WB Rabbit AA 41-56 Log in to see Polyclonal
0.124960825 ABIN2163279 IHC ELISA WB Rabbit IgG Thr125 Log in to see Polyclonal
0.124960825 ABIN2163278 IF ELISA WB Rabbit IgG Tyr153 Log in to see Polyclonal
0.124960825 ABIN2163280 IF IHC ELISA WB Rabbit IgG Thr125 Log in to see Polyclonal
0.124960825 ABIN3023291 WB Mouse IgG Log in to see
0.124960825 ABIN3182484 ELISA IHC WB Rabbit IgG pThr125 Log in to see Polyclonal
0.124960825 ABIN3183673 ELISA IF IHC WB Rabbit IgG Ser20 Log in to see Polyclonal
0.124960825 ABIN1858258 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 1-200 Log in to see Polyclonal

Top referenced anti-Caspase 9 antibody for Western Blotting

Similar anti-Caspase 9 Antibodies

Application / Reactivity Mouse (Murine) Rat (Rattus)
Simple Western (SimWes) 2 Antibodies 2 Antibodies
Western Blotting (WB) 100 Antibodies 96 Antibodies
Immunoprecipitation (IP) 15 Antibodies 15 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies 18 Antibodies
Immunohistochemistry (IHC) 49 Antibodies 46 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 12 Antibodies
Immunofluorescence (IF) 21 Antibodies 26 Antibodies
Immunocytochemistry (ICC) 13 Antibodies 12 Antibodies

General

Antigen Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(421), (121), (116), (21), (13), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(419), (48), (1)
Clonality
Polyclonal
Conjugate This Caspase 9 antibody is un-conjugated
(11), (10), (8), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3)
Application Western Blotting (WB)
(385), (161), (131), (79), (72), (67), (49), (36), (20), (13), (13), (6), (2), (2), (2)
Pubmed 1 reference available
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Product Details anti-Caspase 9 Antibody

Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN223131) Images
Blocking Peptide Blocking peptide for this product available: ABIN412661
Specificity The antibody detects 45 kDa procaspase-9, 35/37 kDa cleaved large fragment and the 25 kDa fully cleaved caspase-9.
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 345 of mouse caspase-9
Isotype IgG

Target Details Caspase 9

Product Details anti-Caspase 9 Antibody Application Details Handling References for anti-Caspase 9 Antibody (ABIN223131) Images back to top
Antigen
Alternative Name Caspase-9 (CASP9 Antibody Abstract)
Background Caspase-9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis, cytochrome c released from mitochondria associates with procaspase-9/Apaf-1. The complex processes procaspase-9 into a large subunit (35 kDa or 17 kDa) and small (10 kDa) by self-cleavage at D315. Activated caspase-9 further cleaves other caspase members including caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage, procaspase-9 can also be cleaved in vivo by caspase-3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway.
Synonyms: CASP-9 , EC 3.4.22.62 , ICE-like apoptotic protease 6 ,ICE-LAP6 ,Apoptotic protease Mch-6 ,Apoptotic protease-activating factor 3 , Apaf-3
Gene ID 12371
Research Area Apoptosis/Necrosis, Cancer
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling References for anti-Caspase 9 Antibody (ABIN223131) Images back to top
Application Notes Western blotting (0.5-4 μg/mL).
Mouse small intestine and rat kidney tissue lysates can be used as positive controls.
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: China

Restrictions For Research Use only

Handling

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details References for anti-Caspase 9 Antibody (ABIN223131) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) protein A purified rabbit anti-caspase-9 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-Caspase 9 Antibody (ABIN223131)

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling Images back to top
Product cited in:

Woods, Burns, Bradford, Ross, Kosyk, Swenberg, Cunningham, Rusyn: "WY-14,643 induced cell proliferation and oxidative stress in mouse liver are independent of NADPH oxidase." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 98, Issue 2, pp. 366-74, 2007

Images

Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN223131) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 9 antibody (Caspase 9, Apoptosis-Related Cysteine Peptidase) (ABIN223131) Western blot analysis of Caspase-9 expression with mouse small intestine tissue lysate.