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|Application / Reactivity||Mouse (Murine)||Rat (Rattus)|
|Simple Western (SimWes)||2 Antibodies||2 Antibodies|
|Western Blotting (WB)||102 Antibodies||98 Antibodies|
|Immunoprecipitation (IP)||15 Antibodies||15 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||19 Antibodies||18 Antibodies|
|Immunohistochemistry (IHC)||49 Antibodies||46 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||5 Antibodies||5 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||12 Antibodies||11 Antibodies|
|Immunofluorescence (IF)||21 Antibodies||26 Antibodies|
|Immunocytochemistry (ICC)||13 Antibodies||12 Antibodies|
|Antigen||Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9) Antibodies|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Caspase 9 antibody is un-conjugated Alternatives|
Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-Caspase 9 AntibodyTarget Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN223131) Images
|Blocking Peptide||Blocking peptide for this product available: ABIN412661|
|Specificity||The antibody detects 45 kDa procaspase-9, 35/37 kDa cleaved large fragment and the 25 kDa fully cleaved caspase-9.|
|Immunogen||Synthetic peptide surrounding amino acid 345 of mouse caspase-9|
Target Details Caspase 9Product Details anti-Caspase 9 Antibody Application Details Handling References for anti-Caspase 9 Antibody (ABIN223131) Images back to top
|Alternative Name||Caspase-9 (CASP9 Antibody Abstract)|
Caspase-9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis, cytochrome c released from mitochondria associates with procaspase-9/Apaf-1. The complex processes procaspase-9 into a large subunit (35 kDa or 17 kDa) and small (10 kDa) by self-cleavage at D315. Activated caspase-9 further cleaves other caspase members including caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage, procaspase-9 can also be cleaved in vivo by caspase-3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway.
Synonyms: CASP-9 , EC 188.8.131.52 , ICE-like apoptotic protease 6 ,ICE-LAP6 ,Apoptotic protease Mch-6 ,Apoptotic protease-activating factor 3 , Apaf-3
|Research Area||Apoptosis/Necrosis, Cancer|
|Pathways||MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-Caspase 9 Antibody Target Details Caspase 9 Handling References for anti-Caspase 9 Antibody (ABIN223131) Images back to top
Western blotting (0.5-4 μg/mL).
Mouse small intestine and rat kidney tissue lysates can be used as positive controls.
However, the optimal conditions should be determined individually.
Country of Animal Origin: China
|Restrictions||For Research Use only|
HandlingProduct Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details References for anti-Caspase 9 Antibody (ABIN223131) Images back to top
|Buffer||100 μg (0.2 mg/mL) protein A purified rabbit anti-caspase-9 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.|
|Precaution of Use||This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
|Storage Comment||For long-term storage, aliquot and freeze at -70 °C.|
|Expiry Date||12 months|
References for anti-Caspase 9 Antibody (ABIN223131)Product Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling Images back to top
|Product cited in:||
Woods, Burns, Bradford, Ross, Kosyk, Swenberg, Cunningham, Rusyn: "WY-14,643 induced cell proliferation and oxidative stress in mouse liver are independent of NADPH oxidase." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 98, Issue 2, pp. 366-74, 2007
ImagesProduct Details anti-Caspase 9 Antibody Target Details Caspase 9 Application Details Handling References for anti-Caspase 9 Antibody (ABIN223131) back to top