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anti-Cow (Bovine) CDC42 antibody for Western Blotting

Recommended CDC42 Antibody (supplied by: Log in to see )

Antigen
Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42) Antibodies
  • AI747189
  • AU018915
  • CDC42
  • Cdc42
  • cdc42
  • cdc42a
  • Cdc42Dm
  • cdc42hs
  • CDC42Hs
  • CG12530
  • D-Cdc42
  • D-CDC42
  • D-cdc42
  • DCDC42
  • DCdc42
  • Dcdc42
  • Dm Cdc42
  • Dmcdc42
  • DmCDC42
  • Dmel\\CG12530
  • g25k
  • G25K
  • wu:fb07d08
  • wu:fi33d02
  • wu:fj62g06
  • Xcdc42
  • zgc:55427
  • zgc:63918
  • zgc:86687
Epitope
AA 121-138, Middle Region
14
13
13
12
12
6
5
5
4
2
2
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
99
45
44
7
6
4
3
2
1
1
Host
Rabbit
80
10
10
Clonality
Polyclonal
Conjugate
This CDC42 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
81
45
18
18
11
7
4
3
3
2
1
1
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN3044385
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN2705837 WB Rabbit Center Log in to see Polyclonal
0.0011728023 ABIN965836 ICC IHC (fro) IHC (p) WB AA 121-138 Log in to see Polyclonal 2
0.0011728023 ABIN4296947 IHC WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN3030463 IHC (p) WB Rabbit IgG Middle Region Log in to see Polyclonal

Top referenced anti-CDC42 antibody for Western Blotting

  • Human Polyclonal CDC42 Primary Antibody for ICC, IHC (fro) - ABIN965836 (2 Publications): Shinjo, Koland, Hart, Narasimhan, Johnson, Evans, Cerione: Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42. in Proceedings of the National Academy of Sciences of the United States of America 1991 (PubMed)

Similar anti-CDC42 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine)
Western Blotting (WB) 83 Antibodies 34 Antibodies 33 Antibodies 5 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 5 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies 7 Antibodies 7 Antibodies 3 Antibodies
Immunohistochemistry (IHC) 19 Antibodies 10 Antibodies 10 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies 3 Antibodies 3 Antibodies

General

Antigen Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42) Antibodies
Epitope AA 121-138, Middle Region
(14), (13), (13), (12), (12), (6), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(99), (45), (44), (7), (6), (4), (3), (2), (1), (1)
Host Rabbit
(80), (10), (10)
Clonality
Polyclonal
Conjugate This CDC42 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(81), (45), (18), (18), (11), (7), (4), (3), (3), (2), (1), (1)
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Product Details anti-CDC42 Antibody

Target Details CDC42 Application Details Handling Images
Sequence DDPSTIEKLA KNKQKPIT
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Cell division control protein 42 homolog(CDC42) detection. Tested with WB, IHC-P in Human,Bovin,Mouse,Rat.
Gene Name: cell division cycle 42(GTP binding protein, 25 kDa)
Protein Name: Cell division control protein 42 homolog(Cdc42 homolog)
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human CDC42(121-138aa DDPSTIEKLAKNKQKPIT), identical to the related mouse and rat sequences.
Isotype IgG

Target Details CDC42

Product Details anti-CDC42 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CDC42 (CDC42 Antibody Abstract)
Background Cell division control protein 42 homolog also known as CDC42 is a protein involved in regulation of the cell cycle. In humans, CDC42 is encoded by the CDC42 gene. CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein(N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.
UniProt P60953
Research Area Cancer, Cell Cycle, Kinases/Phosphatases
Pathways MAPK Signaling, Microtubule Dynamics, RTK Signaling, WNT Signaling, TCR Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-CDC42 Antibody Target Details CDC42 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Bovine, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-CDC42 Antibody Target Details CDC42 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-CDC42 Antibody Target Details CDC42 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CDC42 antibody (Cell Division Cycle 42 (GTP Binding Protein, 25kDa)) (AA 121-138) (ABIN3044385) Anti-CDC42 antibody, Western blotting Lane 1: Recombinant Human CDC42 Protein 10ng La...
Immunohistochemistry (IHC) image for anti-CDC42 antibody (Cell Division Cycle 42 (GTP Binding Protein, 25kDa)) (AA 121-138) (ABIN3044385) Anti-CDC42 antibody, IHC(P) IHC(P): Human Mammary Cancer Tissue