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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Cow (Bovine)|
|Western Blotting (WB)||82 Antibodies||33 Antibodies||32 Antibodies||5 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies||2 Antibodies||2 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||8 Antibodies||6 Antibodies||6 Antibodies||3 Antibodies|
|Immunohistochemistry (IHC)||18 Antibodies||9 Antibodies||9 Antibodies||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies||2 Antibodies||2 Antibodies|
|Antigen||Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42) Antibodies|
|Epitope||AA 121-138, Middle Region Alternatives|
|Reactivity||Cow (Bovine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CDC42 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-CDC42 AntibodyTarget Details CDC42 Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Cell division control protein 42 homolog(CDC42) detection. Tested with WB, IHC-P in Human,Bovin,Mouse,Rat.
Gene Name: cell division cycle 42(GTP binding protein, 25 kDa)
Protein Name: Cell division control protein 42 homolog(Cdc42 homolog)
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human CDC42(121-138aa DDPSTIEKLAKNKQKPIT), identical to the related mouse and rat sequences.|
Target Details CDC42Product Details anti-CDC42 Antibody Application Details Handling Images back to top
|Alternative Name||CDC42 (CDC42 Antibody Abstract)|
|Background||Cell division control protein 42 homolog also known as CDC42 is a protein involved in regulation of the cell cycle. In humans, CDC42 is encoded by the CDC42 gene. CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein(N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.|
|Research Area||Cancer, Cell Cycle, Kinases/Phosphatases|
|Pathways||MAPK Signaling, Microtubule Dynamics, RTK Signaling, WNT Signaling, TCR Signaling, EGFR Signaling Pathway|
Application DetailsProduct Details anti-CDC42 Antibody Target Details CDC42 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Bovine, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-CDC42 Antibody Target Details CDC42 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-CDC42 Antibody Target Details CDC42 Application Details Handling back to top