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anti-Rat (Rattus) CDC42 antibody for Immunofluorescence

Recommended CDC42 Antibody (supplied by: Log in to see )

Antigen
Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42) Antibodies
  • AI747189
  • AU018915
  • CDC42
  • Cdc42
  • cdc42
  • cdc42a
  • Cdc42Dm
  • cdc42hs
  • CDC42Hs
  • CG12530
  • D-Cdc42
  • D-CDC42
  • D-cdc42
  • DCDC42
  • DCdc42
  • Dcdc42
  • Dm Cdc42
  • Dmcdc42
  • DmCDC42
  • Dmel\\CG12530
  • g25k
  • G25K
  • wu:fb07d08
  • wu:fi33d02
  • wu:fj62g06
  • Xcdc42
  • zgc:55427
  • zgc:63918
  • zgc:86687
Epitope
AA 1-191
14
13
13
12
11
5
5
5
5
3
2
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
97
42
42
7
5
4
3
2
1
1
Host
Mouse
79
9
9
Clonality
Monoclonal
Conjugate
This CDC42 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
78
44
18
17
11
8
4
3
2
2
1
1
Supplier
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Catalog No. ABIN968255
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010868901 ABIN968256 IF WB Mouse IgG1 AA 1-191 Log in to see 44-CDC42 5

Top referenced anti-CDC42 antibody for Immunofluorescence

Similar anti-CDC42 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine)
Western Blotting (WB) 81 Antibodies 31 Antibodies 31 Antibodies 5 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 5 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies 7 Antibodies 7 Antibodies
Immunohistochemistry (IHC) 18 Antibodies 9 Antibodies 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies 3 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies 11 Antibodies

General

Antigen Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42) Antibodies
Epitope AA 1-191
(14), (13), (13), (12), (11), (5), (5), (5), (5), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(97), (42), (42), (7), (5), (4), (3), (2), (1), (1)
Host Mouse
(79), (9), (9)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CDC42 antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(78), (44), (18), (17), (11), (8), (4), (3), (2), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-CDC42 Antibody

Target Details CDC42 Application Details Handling References for anti-CDC42 Antibody (ABIN968255) Images
Cross-Reactivity Rat (Rattus), Dog (Canine), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human CDC42 aa. 1-191
Clone 44-CDC42
Isotype IgG1

Target Details CDC42

Product Details anti-CDC42 Antibody Application Details Handling References for anti-CDC42 Antibody (ABIN968255) Images back to top
Antigen
Alternative Name Cdc42 (CDC42 Antibody Abstract)
Background Rho family members are small GTP binding proteins that serve as molecular switches for a number of biological processes. They cycle between active GTP-bound and inactive GDP-bound states. CDC42 is a Rho family protein that was identified in membranes of human platelets and placenta. It is the homologue of CDC42Sc, which regulates initiation of bud-site assembly in Saccharomyces cerevisiae. CDC42 regulates the function of the mammalian actin cytoskeleton, allowing for efficient cytokinesis and cell morphogenesis. CDC42 and Rac1, a Ras-related GTPase, activate MEKK1, a JNK kinase kinase, which leads to the activation of several downstream components of the MAP kinase cascade leading to activation of PAK65, a CDC42- and Rac-binding protein. PAK65 interacts with CDC42/Rac1, mediates their interaction with MEKK1, and enhances MEKK1 catalytic activity. Chronic activation of CDC42 has been shown to induce malignant cellular transformation. Due to sequence homology among various small GTPase family members, potential cross-reactivity could be observed with this antibody.
Molecular Weight 22 kDa
Pathways MAPK Signaling, Microtubule Dynamics, RTK Signaling, WNT Signaling, TCR Signaling, EGFR Signaling Pathway

Application Details

Product Details anti-CDC42 Antibody Target Details CDC42 Handling References for anti-CDC42 Antibody (ABIN968255) Images back to top
Comment

Related Products: ABIN967389, ABIN968545

Restrictions For Research Use only

Handling

Product Details anti-CDC42 Antibody Target Details CDC42 Application Details References for anti-CDC42 Antibody (ABIN968255) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-CDC42 Antibody (ABIN968255)

Product Details anti-CDC42 Antibody Target Details CDC42 Application Details Handling Images back to top
Product cited in:

Habas, Dawid, He: "Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation." in: Genes & development, Vol. 17, Issue 2, pp. 295-309, 2003

Brandt, Gimona, Hillmann, Haller, Mischak: "Protein kinase C induces actin reorganization via a Src- and Rho-dependent pathway." in: The Journal of biological chemistry, Vol. 277, Issue 23, pp. 20903-10, 2002

Zugasti, Rul, Roux, Peyssonnaux, Eychene, Franke, Fort, Hibner: "Raf-MEK-Erk cascade in anoikis is controlled by Rac1 and Cdc42 via Akt." in: Molecular and cellular biology, Vol. 21, Issue 19, pp. 6706-17, 2001

Background publications

Shinjo, Koland, Hart, Narasimhan, Johnson, Evans, Cerione: "Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 24, pp. 9853-7, 1991

Munemitsu, Innis, Clark, McCormick, Ullrich, Polakis: "Molecular cloning and expression of a G25K cDNA, the human homolog of the yeast cell cycle gene CDC42." in: Molecular and cellular biology, Vol. 10, Issue 11, pp. 5977-82, 1990

Images

Product Details anti-CDC42 Antibody Target Details CDC42 Application Details Handling References for anti-CDC42 Antibody (ABIN968255) back to top
Supplier Images
Western Blotting (WB) image for anti-CDC42 antibody (Cell Division Cycle 42 (GTP Binding Protein, 25kDa)) (AA 1-191) (ABIN968255) Western blot analysis of CDC42 on rat brain lysate. Lane 1: 1:250, lane 2: 1:500, lan...