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anti-Human Cortactin antibody for Immunoprecipitation

Recommended Cortactin Antibody (supplied by: Log in to see )

Antigen
Cortactin (CTTN) Antibodies
  • 1110020L01Rik
  • CG3637
  • cortactin
  • Cttn
  • CTTN
  • cttn
  • CTTN1
  • Cttnb
  • Dmel\CG3637
  • EMS1
  • Ems1
  • ems1
  • P85.25
Reactivity
Dog (Canine), Human
188
115
66
7
7
5
3
2
2
1
1
1
1
1
1
Host
Mouse
176
25
16
Clonality
Monoclonal
Conjugate
This Cortactin antibody is un-conjugated
4
4
4
4
4
4
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
182
97
89
29
18
10
8
3
3
1
1
1
1
Supplier
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Catalog No. ABIN967709
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089556724 ABIN967710 IHC (f) IF IP WB Mouse IgG1 Log in to see 30-Cortactin 6
0.00089556724 ABIN2704676 IC IF IP IHC WB Rabbit C-Term, pTyr466 Log in to see Polyclonal
0.00089556724 ABIN2705927 IP IHC WB Rabbit Center Log in to see Polyclonal
0.00089556724 ABIN2705928 IP IHC WB Rabbit pTyr421 Log in to see Polyclonal
0.00089556724 ABIN189263 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see EP1922Y
0.00089556724 ABIN1978163 IP WB Rabbit IgG AA 250-300 Log in to see Polyclonal
0.00089556724 ABIN1978164 IF IP IHC (p) WB Rabbit IgG AA 450-500 Log in to see Polyclonal

Top referenced anti-Cortactin antibody for Immunoprecipitation

Similar anti-Cortactin Antibodies

Application / Reactivity Human Dog (Canine)
ELISA 80 Antibodies 1 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 1 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 20 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 87 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 165 Antibodies

General

Antigen Cortactin (CTTN) Antibodies
Reactivity Dog (Canine), Human
(188), (115), (66), (7), (7), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(176), (25), (16)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cortactin antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(182), (97), (89), (29), (18), (10), (8), (3), (3), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Cortactin Antibody

Target Details Cortactin Application Details Handling References for anti-Cortactin Antibody (ABIN967709) Images
Cross-Reactivity Human, Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Chicken Cortactin
Clone 30-Cortactin
Isotype IgG1

Target Details Cortactin

Product Details anti-Cortactin Antibody Application Details Handling References for anti-Cortactin Antibody (ABIN967709) Images back to top
Antigen
Alternative Name Cortactin (CTTN Antibody Abstract)
Background Cortactin is a protein which is represented by two structurally related isoforms of 80 kDa and 85 kDa. Sequence analysis of this protein reveals the presence of a carboxy-terminal SH3 domain, as well as six internal tandem repeats of a 37 amino acid sequence. It is able to bind to F-actin via this N-terminal repeat region, and colocalizes with actin in the cell. Cortactin was originally described as a protein that is tyrosine-phosphorylated in cells transformed by the src oncogene, in which it localizes to rosettes or podosomes. It has since been shown that other stimuli, such as bacterial invasion, treatment with growth factors, and integrin-mediated cell adhesion, are also capable of inducing phosphorylation of cortactin. Some additional evidence indicates that cortactin may actually be a substrate of syk, with src acting as an upstream signaling molecule. This antibody is routinely tested by western blot analysis.
Molecular Weight 85/80 kDa
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments
Pathways MAPK Signaling

Application Details

Product Details anti-Cortactin Antibody Target Details Cortactin Handling References for anti-Cortactin Antibody (ABIN967709) Images back to top
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-Cortactin Antibody Target Details Cortactin Application Details References for anti-Cortactin Antibody (ABIN967709) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-Cortactin Antibody (ABIN967709)

Product Details anti-Cortactin Antibody Target Details Cortactin Application Details Handling Images back to top
Product cited in:

Lavoie, Champagne, Gingras, Robert: "Adenovirus E4 open reading frame 4-induced apoptosis involves dysregulation of Src family kinases." in: The Journal of cell biology, Vol. 150, Issue 5, pp. 1037-56, 2000

Nakahara, Mueller, Nomizu, Yamada, Yeh, Chen: "Activation of beta1 integrin signaling stimulates tyrosine phosphorylation of p190RhoGAP and membrane-protrusive activities at invadopodia." in: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 9-12, 1998

Dehio, Prévost, Sansonetti: "Invasion of epithelial cells by Shigella flexneri induces tyrosine phosphorylation of cortactin by a pp60c-src-mediated signalling pathway." in: The EMBO journal, Vol. 14, Issue 11, pp. 2471-82, 1995

Wu, Parsons: "Cortactin, an 80/85-kilodalton pp60src substrate, is a filamentous actin-binding protein enriched in the cell cortex." in: The Journal of cell biology, Vol. 120, Issue 6, pp. 1417-26, 1993

Wu, Reynolds, Kanner, Vines, Parsons: "Identification and characterization of a novel cytoskeleton-associated pp60src substrate." in: Molecular and cellular biology, Vol. 11, Issue 10, pp. 5113-24, 1991

Images

Product Details anti-Cortactin Antibody Target Details Cortactin Application Details Handling References for anti-Cortactin Antibody (ABIN967709) back to top
Supplier Images
Western Blotting (WB) image for anti-Cortactin antibody (CTTN) (ABIN967709) Western blot analysis of Cortactin on a A431 lysate. Lane 1: 1:1000, lane 2: 1:2000, ...
Immunofluorescence (IF) image for anti-Cortactin antibody (CTTN) (ABIN967709) Immunofluoresence staining of NIH-3T3 cells.