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anti-Mouse (Murine) Cortactin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Cortactin Antibody (supplied by: Log in to see )

Antigen
Cortactin (CTTN) Antibodies
  • 1110020L01Rik
  • CG3637
  • cortactin
  • Cttn
  • CTTN
  • cttn
  • CTTN1
  • Cttnb
  • Dmel\CG3637
  • EMS1
  • Ems1
  • ems1
  • P85.25
Reactivity
Human, Mouse (Murine), Rat (Rattus)
179
105
59
8
7
5
3
2
2
1
1
1
1
1
1
Host
Rabbit
165
25
15
Clonality
Polyclonal
Conjugate
This Cortactin antibody is un-conjugated
4
4
4
4
4
4
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
170
89
85
21
16
9
7
3
3
2
1
1
1
Supplier
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Catalog No. ABIN498635
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.3452421 ABIN498755 IHC (p) WB Rabbit pTyr421 Log in to see Polyclonal
0.3452421 ABIN498636 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0008944301 ABIN197530 IHC (p) Rabbit Tyr421 Log in to see Polyclonal 4
0.0008944301 ABIN197532 IHC (p) WB Rabbit Tyr466 Log in to see Polyclonal 3
0.0008944301 ABIN1106822 EIA IHC (p) WB Mouse IgG1 Log in to see 4C6 2
0.0008944301 ABIN2854674 ICC IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.0008944301 ABIN189263 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see EP1922Y
0.0008944301 ABIN2888968 IHC (p) ELISA WB Rabbit IgG AA 441-490 Log in to see Polyclonal
0.0008944301 ABIN2888389 IHC (p) ELISA WB Rabbit IgG pTyr466 Log in to see Polyclonal
0.0008944301 ABIN447054 ICC IF IHC (p) IHC WB Rabbit Tyr460 Log in to see Polyclonal
0.0008944301 ABIN680293 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0008944301 ABIN4218895 IHC (p) Rabbit pTyr466 Log in to see Polyclonal
0.0008944301 ABIN2718800 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-Cortactin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-Cortactin Antibodies

Application / Reactivity Mouse (Murine) Human Rat (Rattus)
Western Blotting (WB) 86 Antibodies 156 Antibodies 57 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 8 Antibodies 4 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies 14 Antibodies 8 Antibodies
Immunohistochemistry (IHC) 81 Antibodies 51 Antibodies 27 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 18 Antibodies 12 Antibodies

General

Antigen Cortactin (CTTN) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(179), (105), (59), (8), (7), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(165), (25), (15)
Clonality
Polyclonal
Conjugate This Cortactin antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(170), (89), (85), (21), (16), (9), (7), (3), (3), (2), (1), (1), (1)
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Product Details anti-Cortactin Antibody

Target Details Cortactin Application Details Handling Images
Specificity This antibody detects endogenous levels of Cortactin protein. (region surrounding Pro415)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography using epitope-specific immunogen
Purity > 95 % (by SDS-PAGE)

Target Details Cortactin

Product Details anti-Cortactin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cortactin (CTTN Antibody Abstract)
Background Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.Synonyms: Amplaxin, CTTN, EMS1, Oncogene EMS1
Molecular Weight approx. 85 kDa
Gene ID 2017
NCBI Accession NP_005222
UniProt Q14247
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments
Pathways MAPK Signaling

Application Details

Product Details anti-Cortactin Antibody Target Details Cortactin Handling Images back to top
Application Notes Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Cortactin Antibody Target Details Cortactin Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-Cortactin Antibody Target Details Cortactin Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Cortactin antibody (CTTN) (ABIN498635) Immunohistochemistry analyzes of Cortactin antibody in paraffin-embedded human...
Western Blotting (WB) image for anti-Cortactin antibody (CTTN) (ABIN498635) Western blot analyzes of Cortactin antibody in extracts from HeLa cells.