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|Antigen||Cortactin (CTTN) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Cortactin antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Cortactin AntibodyTarget Details Cortactin Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Cortactin protein only when phosphorylated at Tyr421.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity Chromatography using epitope-specific immunogen.|
Target Details CortactinProduct Details anti-Cortactin Antibody Application Details Handling Images back to top
|Alternative Name||Cortactin (CTTN Antibody Abstract)|
|Background||Cortactin (also designated Ems-1) is a filamentous Actin (FActin) binding protein. It has also been shown to be a substrate for Src p60. Cortactin contains tandem 37 amino acid repeats at the amino-terminus and an SH3 domain at the carboxy-terminus. The tandem repeats appear to be necessary for F-Actin binding. Tyrosine phosphorylation of Cortactin by Src p60 results in diminished F-Actin binding to Cortactin and reduced F-Actin cross-linking activity. Cortactin has also been shown to be phosphorylated in response to FGF-1. Cortactin exhibits abundant expression in megakaryocytes and platelets, and it may play a role in the maturation of megakaryocytes.Synonyms: Amplaxin, CTTN, EMS1, Oncogene EMS1|
|Molecular Weight||approx. 85.0 kDa|
|Research Area||Cancer, Cell Cycle, Cytoskeleton, Microfilaments|
Application DetailsProduct Details anti-Cortactin Antibody Target Details Cortactin Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Cortactin Antibody Target Details Cortactin Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-Cortactin Antibody Target Details Cortactin Application Details Handling back to top