You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Rat (Rattus) Cortactin antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Cortactin Antibody (supplied by: Log in to see )

Antigen
Cortactin (CTTN) Antibodies
  • 1110020L01Rik
  • CG3637
  • cortactin
  • Cttn
  • CTTN
  • cttn
  • CTTN1
  • Cttnb
  • Dmel\CG3637
  • EMS1
  • Ems1
  • ems1
  • P85.25
Reactivity
Human, Mouse (Murine), Rat (Rattus)
188
114
65
8
7
5
3
2
2
1
1
1
1
1
1
Host
Rabbit
175
26
16
Clonality
Polyclonal
Conjugate
This Cortactin antibody is un-conjugated
4
4
4
4
4
4
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
182
97
89
28
19
10
9
3
3
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN498635
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.34288716 ABIN498755 IHC (p) WB Rabbit pTyr421 Log in to see Polyclonal
0.0008982679 ABIN2854674 ICC IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.0008982679 ABIN189263 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see EP1922Y
0.0008982679 ABIN447054 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN1733371 IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0008982679 ABIN680293 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0008982679 ABIN4300123 ELISA IHC IHC (p) WB Rabbit C-Term, pTyr466 Log in to see Polyclonal
0.0008982679 ABIN4300120 IHC IHC (p) WB Rabbit pTyr421 Log in to see Polyclonal
0.0008982679 ABIN4300126 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN4300118 IHC IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN2718800 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal

Similar anti-Cortactin Antibodies

Application / Reactivity Mouse (Murine) Human Rat (Rattus)
Western Blotting (WB) 93 Antibodies 165 Antibodies 62 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 8 Antibodies 4 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies 18 Antibodies 12 Antibodies
Immunohistochemistry (IHC) 87 Antibodies 56 Antibodies 31 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 20 Antibodies 13 Antibodies

General

Antigen Cortactin (CTTN) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(188), (114), (65), (8), (7), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(175), (26), (16)
Clonality
Polyclonal
Conjugate This Cortactin antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(182), (97), (89), (28), (19), (10), (9), (3), (3), (2), (1), (1), (1)
Supplier Log in to see

Product Details anti-Cortactin Antibody

Target Details Cortactin Application Details Handling Images
Specificity This antibody detects endogenous levels of Cortactin protein. (region surrounding Pro415)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography using epitope-specific immunogen
Purity > 95 % (by SDS-PAGE)

Target Details Cortactin

Product Details anti-Cortactin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Cortactin (CTTN Antibody Abstract)
Background Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.Synonyms: Amplaxin, CTTN, EMS1, Oncogene EMS1
Molecular Weight approx. 85 kDa
Gene ID 2017
NCBI Accession NP_005222
UniProt Q14247
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments
Pathways MAPK Signaling

Application Details

Product Details anti-Cortactin Antibody Target Details Cortactin Handling Images back to top
Application Notes Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Cortactin Antibody Target Details Cortactin Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-Cortactin Antibody Target Details Cortactin Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Cortactin antibody (CTTN) (ABIN498635) Immunohistochemistry analyzes of Cortactin antibody in paraffin-embedded human...
Western Blotting (WB) image for anti-Cortactin antibody (CTTN) (ABIN498635) Western blot analyzes of Cortactin antibody in extracts from HeLa cells.