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|Antigen||Death-Associated Protein Kinase 1 (DAPK1) Antibodies|
|Epitope||AA 694-947 Alternatives|
|Conjugate||This DAP Kinase 1 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|2 references available|
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Product Details anti-DAP Kinase 1 AntibodyTarget Details DAP Kinase 1 Application Details Handling References for anti-DAP Kinase 1 antibody (ABIN967885) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human DAP Kinase aa. 694-947|
Target Details DAP Kinase 1Product Details anti-DAP Kinase 1 Antibody Application Details Handling References for anti-DAP Kinase 1 antibody (ABIN967885) Images back to top
|Alternative Name||DAP Kinase (DAPK1 Antibody Abstract)|
|Background||Chronic exposure to extracellular signals such as interferons induce the inhibition of cell proliferation followed by cell death. The gene for DAP Kinase (Death Associated Protein Kinase) was identified using a novel approach named Technical Knockout. Briefly, Hela cells were transfected with an antisense cDNA expression library, then exposed to Interferon-gamma. The surviving cells, with their antisense cDNAs, were rescued and the protecting genes isolated. The DAP Kinase gene encodes a protein of 1423 amino acids, a molecular weight of 160kDa, a kinase domain at its amino terminal region, ankyrin repeats in the middle region, and a death domain at the extreme C-terminus. DAP Kinase phosphorylates at Ser/Thr residues in a Ca2+/Calmodulin-dependent fashion. It has been demonstrated that Ca2+/Calmodulin binds directly to DAP Kinase at its amino terminal region. In addition, immunostaining studies localized DAP Kinase in association with the actin filaments where it may phosphorylate myosin light chain. Thus, this novel cytoskeletal and Ca2+/Calmodulin-dependent protein kinase plays a role in interferon-gamma-induced cell death. This antibody is routinely tested by western blot analysis.|
|Molecular Weight||160 kDa|
|Pathways||MAPK Signaling, Interferon-gamma Pathway|
Application DetailsProduct Details anti-DAP Kinase 1 Antibody Target Details DAP Kinase 1 Handling References for anti-DAP Kinase 1 antibody (ABIN967885) Images back to top
Related Products: ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-DAP Kinase 1 Antibody Target Details DAP Kinase 1 Application Details References for anti-DAP Kinase 1 antibody (ABIN967885) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20° C.|
References for anti-DAP Kinase 1 antibody (ABIN967885)Product Details anti-DAP Kinase 1 Antibody Target Details DAP Kinase 1 Application Details Handling Images back to top
|Product cited in:||
Cohen, Feinstein, Kimchi: "DAP-kinase is a Ca2+/calmodulin-dependent, cytoskeletal-associated protein kinase, with cell death-inducing functions that depend on its catalytic activity." in: The EMBO journal, Vol. 16, Issue 5, pp. 998-1008, 1997
Deiss, Feinstein, Berissi, Cohen, Kimchi: "Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death." in: Genes & development, Vol. 9, Issue 1, pp. 15-30, 1995
ImagesProduct Details anti-DAP Kinase 1 Antibody Target Details DAP Kinase 1 Application Details Handling References for anti-DAP Kinase 1 antibody (ABIN967885) back to top