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anti-Hamster EIF4EBP1 antibody for Gel Shift

Recommended EIF4EBP1 Antibody (supplied by: Log in to see )

Antigen
Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
  • 153432_at
  • 43-BP
  • 4E-BP
  • 4E-BP1
  • 4EBP
  • 4e-bp
  • 4eBP
  • 4ebp
  • BcDNA.HL08053
  • BcDNA:HL08053
  • CG8846
  • CT8705
  • Dm 4E-BP
  • Dmel\\CG8846
  • Phas1
  • Thor/EIF4EBP
  • anon-WO0172774.158
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4e-bp
  • d4eBP
  • dthor
  • eIF4E-BP
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • pp20
  • thor
  • EIF4EBP1
  • fc04h09
  • wu:fc04h09
  • zgc:64137
  • 4EBP1
  • BP-1
  • PHAS-I
  • 4e-bp1
  • AA959816
  • CG8846 gene product from transcript CG8846-RA
  • eukaryotic translation initiation factor 4E binding protein 1
  • Thor
  • EIF4EBP1
  • eif4ebp1
  • LOC100230789
  • Eif4ebp1
Epitope
AA 101-118
59
31
27
22
22
20
17
16
15
15
15
14
12
12
10
10
8
8
5
5
5
5
5
4
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Hamster, Human, Mouse (Murine), Rabbit, Rat (Rattus)
452
287
246
21
16
13
7
4
4
4
2
2
1
1
1
1
1
Host
Rabbit
393
95
4
Clonality
Polyclonal
Conjugate
This EIF4EBP1 antibody is un-conjugated
19
16
11
10
8
8
5
5
5
5
5
5
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
Application
Gel Shift (GS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
340
221
201
95
66
28
18
14
14
8
6
5
4
4
2
1
Supplier
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Catalog No. ABIN462434
$ 451.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2467722 GS IHC WB Rabbit AA 101-118 Log in to see Polyclonal

Similar anti-EIF4EBP1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Hamster Rabbit
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 318 Antibodies 189 Antibodies 168 Antibodies 3 Antibodies 7 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 6 Antibodies 3 Antibodies 3 Antibodies
Immunoprecipitation (IP) 6 Antibodies 5 Antibodies 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 93 Antibodies 62 Antibodies 55 Antibodies

General

Antigen Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
Epitope AA 101-118
(59), (31), (27), (22), (22), (20), (17), (16), (15), (15), (15), (14), (12), (12), (10), (10), (8), (8), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Hamster, Human, Mouse (Murine), Rabbit, Rat (Rattus)
(452), (287), (246), (21), (16), (13), (7), (4), (4), (4), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(393), (95), (4)
Clonality
Polyclonal
Conjugate This EIF4EBP1 antibody is un-conjugated
(19), (16), (11), (10), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Gel Shift (GS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(340), (221), (201), (95), (66), (28), (18), (14), (14), (8), (6), (5), (4), (4), (2), (1)
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Product Details anti-EIF4EBP1 Antibody

Target Details EIF4EBP1 Application Details Handling Images
Brand IHC-plus™
Specificity Detects multiple bands at 19-25 kD, corresponding to the apparent molecular mass of the various phosphorylation states of PHAS-I protein on SDS-PAGE immunoblots. This antibody provides a simple read-out for PKB (Akt) activity in vivo through the use of gel-shift assays. Antibody specificity has been confirmed in Western blot peptide competition studies.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%) Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%) Panda (83%).
Purification Immunoaffinity purified
Immunogen An 18 residue synthetic peptide SPEDKRAGGEESQFEMDI based on the human PHAS-I (residues 101-118) and the peptide coupled to KLH. This sequence is identical to rat and mouse PHAS- I over these residues. Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%), Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%), Panda (83%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Target Details EIF4EBP1

Product Details anti-EIF4EBP1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name EIF4EBP1 / 4EBP1 (EIF4EBP1 Antibody Abstract)
Background Name/Gene ID: EIF4EBP1

Synonyms: EIF4EBP1, 4E-BP1, 4EBP1, BP-1, PHAS-I, EIF4E-binding protein 1
Gene ID 1978
UniProt Q13541
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling

Application Details

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Handling Images back to top
Application Notes Approved: GS, IHC-P (5 - 10 μg/mL), WB (4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
Restrictions For Research Use only

Handling

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer BBS, pH 8.4 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)
Handling Advice avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
Storage 4 °C,-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Images

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling back to top
Supplier Images
 image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (AA 101-118) (ABIN462434) Breast, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
 image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (AA 101-118) (ABIN462434) Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)