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anti-Human EIF4EBP1 antibody for Enzyme Immunoassay

Recommended EIF4EBP1 Antibody (supplied by: Log in to see )

Antigen
Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
  • 153432_at
  • 43-BP
  • 4E-BP
  • 4E-BP1
  • 4EBP
  • 4e-bp
  • 4eBP
  • 4ebp
  • BcDNA.HL08053
  • BcDNA:HL08053
  • CG8846
  • CT8705
  • Dm 4E-BP
  • Dmel\\CG8846
  • Phas1
  • Thor/EIF4EBP
  • anon-WO0172774.158
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4e-bp
  • d4eBP
  • dthor
  • eIF4E-BP
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • pp20
  • thor
  • EIF4EBP1
  • fc04h09
  • wu:fc04h09
  • zgc:64137
  • 4EBP1
  • BP-1
  • PHAS-I
  • 4e-bp1
  • AA959816
  • CG8846 gene product from transcript CG8846-RA
  • eukaryotic translation initiation factor 4E binding protein 1
  • Thor
  • EIF4EBP1
  • eif4ebp1
  • LOC100230789
  • Eif4ebp1
Epitope
pThr36
58
31
27
22
22
19
16
16
15
15
15
15
14
12
12
10
8
7
6
5
5
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
432
275
236
20
17
13
9
5
5
5
3
2
2
1
1
1
1
1
Host
Rabbit
376
92
4
Clonality
Polyclonal
Conjugate
This EIF4EBP1 antibody is un-conjugated
19
16
11
10
8
8
5
5
5
5
5
5
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA)
323
205
186
96
66
28
17
14
13
8
7
6
5
3
3
1
Supplier
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Catalog No. ABIN358355
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4620432 EIA IHC (p) WB Rabbit Ig Log in to see Polyclonal 2
1 ABIN950187 EIA WB Rabbit Ig Middle Region Log in to see Polyclonal 2
1 ABIN499179 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 2

Top referenced anti-EIF4EBP1 antibody for Enzyme Immunoassay

Similar anti-EIF4EBP1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 17 Antibodies
ELISA 166 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Gel Shift (GS) 3 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 11 Antibodies
Immunofluorescence (IF) 28 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 53 Antibodies
Immunohistochemistry (IHC) 189 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 93 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Intracellular Staining (ICS) 6 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 300 Antibodies

General

Antigen Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
Epitope pThr36
(58), (31), (27), (22), (22), (19), (16), (16), (15), (15), (15), (15), (14), (12), (12), (10), (8), (7), (6), (5), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(432), (275), (236), (20), (17), (13), (9), (5), (5), (5), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(376), (92), (4)
Clonality
Polyclonal
Conjugate This EIF4EBP1 antibody is un-conjugated
(19), (16), (11), (10), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA)
(323), (205), (186), (96), (66), (28), (17), (14), (13), (8), (7), (6), (5), (3), (3), (1)
Pubmed 2 references available
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Product Details anti-EIF4EBP1 Antibody

Target Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN358355) Images
Specificity This antibody detects 4E-BP1/EIF4EBP1 pThr36. Predicted to cross react with Mouse (100 % Antigen Homology).
Purification Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T36 of human EIF4EBP1.
Isotype Ig

Target Details EIF4EBP1

Product Details anti-EIF4EBP1 Antibody Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN358355) Images back to top
Antigen
Alternative Name EIF4EBP1 / 4E-BP1 (EIF4EBP1 Antibody Abstract)
Background EIF4EBP1 is a member of a family of translation repressor proteins. This protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. It is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.Synonyms: Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-1, PHAS-I, PHAS1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, eIF4E-binding protein 1
Molecular Weight 12449 Da
Gene ID 1978, 9606
UniProt Q13541
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size

Application Details

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Handling References for anti-EIF4EBP1 Antibody (ABIN358355) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details References for anti-EIF4EBP1 Antibody (ABIN358355) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-EIF4EBP1 Antibody (ABIN358355)

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling Images back to top
Background publications

Fonseca, Smith, Lee, MacKintosh, Proud: "PRAS40 is a target for mammalian target of rapamycin complex 1 and is required for signaling downstream of this complex." in: The Journal of biological chemistry, Vol. 282, Issue 34, pp. 24514-24, 2007

Armengol, Rojo, Castellví, Iglesias, Cuatrecasas, Pons, Baselga, Ramón y Cajal: "4E-binding protein 1: a key molecular "funnel factor" in human cancer with clinical implications." in: Cancer research, Vol. 67, Issue 16, pp. 7551-5, 2007

Images

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN358355) back to top
Supplier Images
 image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (pThr36) (ABIN358355) anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) (pThr36) antibody