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anti-Human EIF4EBP1 antibody for Gel Shift

Recommended EIF4EBP1 Antibody (supplied by: Log in to see )

Antigen
Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
  • 4e-bp
  • 4E-BP
  • 4e-bp1
  • 4E-BP1
  • 4ebp
  • 4eBP
  • 4EBP
  • 4EBP1
  • 43-BP
  • 153432_at
  • AA959816
  • anon-WO0172774.158
  • BcDNA.HL08053
  • BcDNA:HL08053
  • BP-1
  • CG8846
  • CT8705
  • d4e-bp
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4eBP
  • Dm 4E-BP
  • Dmel\\CG8846
  • dthor
  • eIF4E-BP
  • EIF4EBP1
  • fc04h09
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • PHAS-I
  • Phas1
  • pp20
  • thor
  • Thor/EIF4EBP
  • wu:fc04h09
  • zgc:64137
Epitope
AA 101-118
59
30
29
22
20
20
17
14
14
13
13
13
12
12
11
10
10
8
8
5
5
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
435
238
190
10
8
5
2
2
1
1
1
Host
Rabbit
383
91
9
Clonality
Polyclonal
Conjugate
This EIF4EBP1 antibody is un-conjugated
20
17
12
11
8
8
5
5
5
5
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Gel Shift (GS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
313
200
192
86
56
26
21
18
14
7
5
4
4
3
2
1
Supplier
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Catalog No. ABIN462434
$ 451.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008711731 ABIN1733953 GS IHC (p) WB Rabbit IgG AA 101-118 Log in to see Polyclonal
0.0008711731 ABIN2467722 GS IHC WB Rabbit AA 101-118 Log in to see Polyclonal

Similar anti-EIF4EBP1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 18 Antibodies
ELISA 173 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 12 Antibodies
Gel Shift (GS) 3 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 27 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 193 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 86 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Intracellular Staining (ICS) 7 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 300 Antibodies

General

Antigen Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
Epitope AA 101-118
(59), (30), (29), (22), (20), (20), (17), (14), (14), (13), (13), (13), (12), (12), (11), (10), (10), (8), (8), (5), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(435), (238), (190), (10), (8), (5), (2), (2), (1), (1), (1)
Host Rabbit
(383), (91), (9)
Clonality
Polyclonal
Conjugate This EIF4EBP1 antibody is un-conjugated
(20), (17), (12), (11), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Gel Shift (GS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(313), (200), (192), (86), (56), (26), (21), (18), (14), (7), (5), (4), (4), (3), (2), (1)
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Product Details anti-EIF4EBP1 Antibody

Target Details EIF4EBP1 Application Details Handling Images
Specificity Detects multiple bands at 19-25 kD, corresponding to the apparent molecular mass of the various phosphorylation states of PHAS-I protein on SDS-PAGE immunoblots. This antibody provides a simple read-out for PKB (Akt) activity in vivo through the use of gel-shift assays. Antibody specificity has been confirmed in Western blot peptide competition studies.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Mouse, Rat, Hamster, Rabbit (100%) Marmoset, Goat, Elephant, Dog, Bat, Bovine, Horse (94%) Panda (83%).
Purification Immunoaffinity Chromatography
Immunogen An 18 residue synthetic peptide SPEDKRAGGEESQFEMDI based on the human PHAS-I (residues 101-118) and the peptide coupled to KLH. This sequence is identical to rat and mouse PHAS- I over these residues.

Target Details EIF4EBP1

Product Details anti-EIF4EBP1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name EIF4EBP1 / 4EBP1 (EIF4EBP1 Antibody Abstract)
Background Standard Gene Symbol: EIF4EBP1, Synonyms: EIF4EBP1, 4E-BP1, 4EBP1, BP-1, PHAS-I, EIF4E-binding protein 1
Gene ID 1978
UniProt Q13541
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling

Application Details

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Handling Images back to top
Application Notes GS, IHC-P (5 - 10 µg/mL), WB (4 µg/mL)
Restrictions For Research Use only

Handling

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Images back to top
Buffer BBS, pH 8.4 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)
Handling Advice avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
Storage 4 °C/-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Images

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling back to top
Supplier Images
 image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (AA 101-118) (ABIN462434) Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
 image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (AA 101-118) (ABIN462434) Breast, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)