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anti-Human EIF4EBP1 antibody for Intracellular Staining

Recommended EIF4EBP1 Antibody (supplied by: Log in to see )

Antigen
Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
  • 4e-bp
  • 4E-BP
  • 4e-bp1
  • 4E-BP1
  • 4ebp
  • 4eBP
  • 4EBP
  • 4EBP1
  • 43-BP
  • 153432_at
  • AA959816
  • anon-WO0172774.158
  • BcDNA.HL08053
  • BcDNA:HL08053
  • BP-1
  • CG8846
  • CT8705
  • d4e-bp
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4eBP
  • Dm 4E-BP
  • Dmel\\CG8846
  • dthor
  • eIF4E-BP
  • EIF4EBP1
  • fc04h09
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • PHAS-I
  • Phas1
  • pp20
  • thor
  • Thor/EIF4EBP
  • wu:fc04h09
  • zgc:64137
Epitope
pThr36
58
30
29
22
21
20
17
14
14
13
13
13
12
12
11
10
10
8
8
6
5
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
438
239
191
11
8
5
2
2
1
1
1
Host
Mouse
386
91
9
Clonality
Monoclonal
Conjugate
This EIF4EBP1 antibody is conjugated to Alexa Fluor 647
20
17
12
11
8
7
5
5
5
5
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Staining (ICS)
317
202
193
89
56
27
21
18
15
6
5
4
4
3
3
1
Supplier
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Supplier Product No.
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Catalog No. ABIN1177022
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN1177023 ICS Alexa Fluor 488 Mouse IgG1, kappa pThr36 Log in to see M31-16 2
0.0008709465 ABIN1177025 ICS Alexa Fluor 647 Mouse IgG1, kappa pThr69 Log in to see M34-273 2
0.0008709465 ABIN1177026 ICS Alexa Fluor 488 Mouse IgG1, kappa pThr69 Log in to see M34-273 2
0.0008709465 ABIN1177024 ICS PE Mouse IgG1, kappa pThr69 Log in to see M34-273 2
0.0008709465 ABIN1177021 ICS PE Mouse IgG1, kappa pThr36 Log in to see M31-16
0.0008709465 ABIN2677045 FACS ICS EFluor 660 Mouse IgG2b kappa pThr36, pThr45 Log in to see V3NTY24

Top referenced anti-EIF4EBP1 antibody for Intracellular Staining

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General

Antigen Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
Epitope pThr36
(58), (30), (29), (22), (21), (20), (17), (14), (14), (13), (13), (13), (12), (12), (11), (10), (10), (8), (8), (6), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(438), (239), (191), (11), (8), (5), (2), (2), (1), (1), (1)
Host Mouse
(386), (91), (9)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EIF4EBP1 antibody is conjugated to Alexa Fluor 647
(20), (17), (12), (11), (8), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Staining (ICS)
(317), (202), (193), (89), (56), (27), (21), (18), (15), (6), (5), (4), (4), (3), (3), (1)
Pubmed 2 references available
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Product Details anti-EIF4EBP1 Antibody

Target Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN1177022) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human 4EBP1 (pT45) Peptide
Clone M31-16
Isotype IgG1, kappa

Target Details EIF4EBP1

Product Details anti-EIF4EBP1 Antibody Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN1177022) Images back to top
Antigen
Alternative Name 4EBP1 (EIF4EBP1 Antibody Abstract)
Background The eukaryotic translation initiation factor 4E-Binding Protein 1 (4EBP1) is a phosphorylated heat- and acid-stable protein (PHAS-I or PHAS-1), and it is regulated by insulin. It is a member of the eIF4E-Binding Protein Family, which also includes the proteins 4EBP2 and 4EBP3. 4EBP1 binds with eukaryotic translation Initiation Factor 4E (eIF4E), which prevents its assembly into the eIF4E complex and inhibits cap-dependent translation. When 4EBP1 is phosphorylated, this binding is disrupted, allowing cap-dependent translation to be activated. Phosphorylation of 4EBP1 is required for protein synthesis, and it mediates the regulation of protein translation by stimuli that signal through the phosphoinositide 3 (PI3) kinase pathway. We found that threonines 36 and 45 (T36/T45) are phosphorylated in resting human peripheral blood mononuclear cells. PI3 kinase inhibitors, such as LY294002 down-regulate the phosphorylation level of 4EBP1 (pT36/pT45). The M31-16 monoclonal antibody recognizes the phosphorylated T36 and T45 of activated human 4EBP1. The orthologous phosphorylation sites in mouse and rat 4EBP1 are T35 and T44.
Synonyms: 4E-BP1. EIF4EBP1, P/OKCL.6, PHAS-I, PHAS-1
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling

Application Details

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Handling References for anti-EIF4EBP1 Antibody (ABIN1177022) Images back to top
Application Notes Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details References for anti-EIF4EBP1 Antibody (ABIN1177022) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-EIF4EBP1 Antibody (ABIN1177022)

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling Images back to top
Product cited in:

Hay, Sonenberg: "Upstream and downstream of mTOR." in: Genes & development, Vol. 18, Issue 16, pp. 1926-45, 2004

Gingras, Raught, Gygi, Niedzwiecka, Miron, Burley, Polakiewicz, Wyslouch-Cieszynska, Aebersold, Sonenberg: "Hierarchical phosphorylation of the translation inhibitor 4E-BP1." in: Genes & development, Vol. 15, Issue 21, pp. 2852-64, 2001