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anti-Human EIF4EBP1 antibody for Immunohistochemistry (Frozen Sections)

Recommended EIF4EBP1 Antibody (supplied by: Log in to see )

Antigen
Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
  • 4e-bp
  • 4E-BP
  • 4e-bp1
  • 4E-BP1
  • 4ebp
  • 4eBP
  • 4EBP
  • 4EBP1
  • 43-BP
  • 153432_at
  • AA959816
  • anon-WO0172774.158
  • BcDNA.HL08053
  • BcDNA:HL08053
  • BP-1
  • CG8846
  • CT8705
  • d4e-bp
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4eBP
  • Dm 4E-BP
  • Dmel\\CG8846
  • dthor
  • eIF4E-BP
  • EIF4EBP1
  • fc04h09
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • PHAS-I
  • Phas1
  • pp20
  • thor
  • Thor/EIF4EBP
  • wu:fc04h09
  • zgc:64137
Epitope
AA 2-118
59
30
29
22
20
20
17
14
14
13
13
13
12
12
11
10
9
8
8
6
5
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
435
238
190
10
8
5
2
2
1
1
1
Host
Rabbit
383
91
9
Clonality
Polyclonal
Conjugate
This EIF4EBP1 antibody is un-conjugated
20
17
12
11
8
8
5
5
5
5
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
313
200
191
86
56
26
21
18
13
7
5
4
4
3
2
1
Supplier
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Catalog No. ABIN1078005
$ 424.60
Plus shipping costs $45.00

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Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
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Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
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General

Antigen Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
Epitope AA 2-118
(59), (30), (29), (22), (20), (20), (17), (14), (14), (13), (13), (13), (12), (12), (11), (10), (9), (8), (8), (6), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(435), (238), (190), (10), (8), (5), (2), (2), (1), (1), (1)
Host Rabbit
(383), (91), (9)
Clonality
Polyclonal
Conjugate This EIF4EBP1 antibody is un-conjugated
(20), (17), (12), (11), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(313), (200), (191), (86), (56), (26), (21), (18), (13), (7), (5), (4), (4), (3), (2), (1)
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Product Details anti-EIF4EBP1 Antibody

Target Details EIF4EBP1 Application Details Handling Images
Sequence MGHHHHHHSG SEF-SGGSSCSQT PSRAIPATRR VVLGDGVQLP PGDYSTTPGG TLFSTTPGGT RIIYDRKFLM ECRNSPVTKT PPRDLPTIPG VTSPSSDEPP MEASQSHLRN SPEDKRAGGE ESQFEMDI
Specificity It has been selected for its ability to recognize EIF4EBP1 in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen Recombinant EIF4EBP1 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against EIF4EBP1.
Isotype IgG

Target Details EIF4EBP1

Product Details anti-EIF4EBP1 Antibody Application Details Handling Images back to top
Antigen
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling

Application Details

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant EIF4EBP1 (Ser2~Ile118) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (AA 2-118) (ABIN1078005) anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) (AA 2-118) antibody