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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||275 Antibodies||137 Antibodies||116 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Intracellular Staining (ICS)||7 Antibodies||4 Antibodies||3 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||61 Antibodies||39 Antibodies||32 Antibodies|
|Immunohistochemistry (IHC)||186 Antibodies||100 Antibodies||96 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies||1 Antibodies|
|Antigen||Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This EIF4EBP1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-EIF4EBP1 AntibodyTarget Details EIF4EBP1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at Thr37.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purity||> 95 % by SDS-PAGE|
Target Details EIF4EBP1Product Details anti-EIF4EBP1 Antibody Application Details Handling Images back to top
|Alternative Name||EIF4EBP1 / 4E-BP1 (EIF4EBP1 Antibody Abstract)|
|Background||The translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regulatory protein 4E-BP1, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to 4E-BP1, designated 4E-BP2, also associates with eIF-4E.Synonyms: Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-1, PHAS-I, PHAS1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, eIF4E-binding protein 1|
|Molecular Weight||approx. 15 to 20 kDa|
|Pathways||MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling|
Application DetailsProduct Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling back to top