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anti-Human EIF4EBP1 antibody for Immunoprecipitation

Recommended EIF4EBP1 Antibody (supplied by: Log in to see )

Antigen
Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
  • 4e-bp
  • 4E-BP
  • 4e-bp1
  • 4E-BP1
  • 4ebp
  • 4eBP
  • 4EBP
  • 4EBP1
  • 43-BP
  • 153432_at
  • AA959816
  • anon-WO0172774.158
  • BcDNA.HL08053
  • BcDNA:HL08053
  • BP-1
  • CG8846
  • CT8705
  • d4e-bp
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4eBP
  • Dm 4E-BP
  • Dmel\\CG8846
  • dthor
  • eIF4E-BP
  • EIF4EBP1
  • fc04h09
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • PHAS-I
  • Phas1
  • pp20
  • thor
  • Thor/EIF4EBP
  • wu:fc04h09
  • zgc:64137
Epitope
C-Term
59
30
29
22
20
20
17
14
14
13
13
13
12
12
11
10
10
8
8
6
5
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
436
238
190
10
8
5
2
2
1
1
1
Host
Rabbit
384
91
9
Clonality
Monoclonal
Conjugate
This EIF4EBP1 antibody is un-conjugated
20
17
12
11
8
8
5
5
5
5
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
314
200
192
87
56
26
21
18
14
7
5
4
4
3
3
1
Supplier
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Catalog No. ABIN189001
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008957968 ABIN221233 IP WB Rabbit IgG pSer112 Log in to see Polyclonal

Top referenced anti-EIF4EBP1 antibody for Immunoprecipitation

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Application / Reactivity Human
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Flow Cytometry (FACS) 12 Antibodies
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Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 27 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 193 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 86 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Intracellular Staining (ICS) 7 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 300 Antibodies

General

Antigen Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
Epitope C-Term
(59), (30), (29), (22), (20), (20), (17), (14), (14), (13), (13), (13), (12), (12), (11), (10), (10), (8), (8), (6), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(436), (238), (190), (10), (8), (5), (2), (2), (1), (1), (1)
Host Rabbit
(384), (91), (9)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EIF4EBP1 antibody is un-conjugated
(20), (17), (12), (11), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(314), (200), (192), (87), (56), (26), (21), (18), (14), (7), (5), (4), (4), (3), (3), (1)
Pubmed 1 reference available
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Product Details anti-EIF4EBP1 Antibody

Target Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN189001) Images
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues near the C-term of human4E-BP1 was used as immunogen.
Clone Y330
Isotype IgG

Target Details EIF4EBP1

Product Details anti-EIF4EBP1 Antibody Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN189001) Images back to top
Antigen
Alternative Name 4E-BP1 (EIF4EBP1 Antibody Abstract)
Background 4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein thatcompete with eIF4G for binding of eiF4E to the mRNA 5' cap structure (1). Binding ofthe 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, thephosphorylated form will not (2). Akt, TOR, MAP kinase, S6 kinase, and Cdc2 areknown kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylatingeither threonines 35, 45, 69 or serine 64. Although, not all phosphorylation eventsequally block the 4EBP1-eIF4E interaction (3-4). Alternate Names: anti-4E BP1 antibody, 4EBP1 antibody, Anti-4E BP1 antibody, Anti-4EBP1 antibody,Anti-BP 1 antibody, Anti-BP1 antibody, Anti-Eukaryotic translation initiation factor 4Ebinding protein 1 antibody, Anti-MGC4316 antibody, Anti-PHAS I antibody, Anti-PHASIantibody, Anti-Phosphorylated heat and acid stable protein regulated by insulin 1antibody, Anti-eIF4E binding protein 1 antibody, Anti-eIF4EBP1 antibody, BP 1 antibody,BP1 antibody, Eukaryotic translation initiation factor 4E binding protein 1 antibody,MGC4316 antibody, PHAS I antibody, PHASI antibody, Phosphorylated heat and acidstable protein regulated by insulin 1 antibody, eIF4E binding protein 1 antibody.
Gene Symbol: EIF4EBP1
Gene ID 1978
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling

Application Details

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Handling References for anti-EIF4EBP1 Antibody (ABIN189001) Images back to top
Application Notes This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 15-20kDa.
Recommended dilutions: Flow Cytometry 1:20, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50-100, Immunoprecipitation 1:50, Western Blot 1:1000-10000
Protocol Protocol specific for eIF4EBP1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details References for anti-EIF4EBP1 Antibody (ABIN189001) Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

References for anti-EIF4EBP1 Antibody (ABIN189001)

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling Images back to top
Product cited in:

Escobar, Frank, Suryawan, Nguyen, Davis: "Amino acid availability and age affect the leucine stimulation of protein synthesis and eIF4F formation in muscle." in: American journal of physiology. Endocrinology and metabolism, Vol. 293, Issue 6, pp. E1615-21, 2007

Images

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN189001) back to top
Supplier Images
Western Blotting (WB) image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (C-Term) (ABIN189001) Western blot analysis on K562 cell lysate using anti-4E-BP1 RabMAb (ABIN189001), dilu...
Immunohistochemistry (IHC) image for anti-EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (C-Term) (ABIN189001) Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti...