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|Application / Reactivity||Human|
|Dot Blot (DB)||18 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Flow Cytometry (FACS)||12 Antibodies|
|Gel Shift (GS)||3 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||11 Antibodies|
|Immunofluorescence (IF)||25 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||46 Antibodies|
|Immunohistochemistry (IHC)||186 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||61 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|Intracellular Staining (ICS)||7 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Western Blotting (WB)||275 Antibodies|
|Antigen||Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies|
|Conjugate||This EIF4EBP1 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|1 reference available|
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Product Details anti-EIF4EBP1 AntibodyTarget Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN189001) Images
|No Cross-Reactivity||Mouse (Murine), Rat (Rattus)|
|Immunogen||A synthetic peptide corresponding to residues near the C-term of human4E-BP1 was used as immunogen.|
Target Details EIF4EBP1Product Details anti-EIF4EBP1 Antibody Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN189001) Images back to top
|Alternative Name||4E-BP1 (EIF4EBP1 Antibody Abstract)|
4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein thatcompete with eIF4G for binding of eiF4E to the mRNA 5' cap structure (1). Binding ofthe 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, thephosphorylated form will not (2). Akt, TOR, MAP kinase, S6 kinase, and Cdc2 areknown kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylatingeither threonines 35, 45, 69 or serine 64. Although, not all phosphorylation eventsequally block the 4EBP1-eIF4E interaction (3-4). Alternate Names: anti-4E BP1 antibody, 4EBP1 antibody, Anti-4E BP1 antibody, Anti-4EBP1 antibody,Anti-BP 1 antibody, Anti-BP1 antibody, Anti-Eukaryotic translation initiation factor 4Ebinding protein 1 antibody, Anti-MGC4316 antibody, Anti-PHAS I antibody, Anti-PHASIantibody, Anti-Phosphorylated heat and acid stable protein regulated by insulin 1antibody, Anti-eIF4E binding protein 1 antibody, Anti-eIF4EBP1 antibody, BP 1 antibody,BP1 antibody, Eukaryotic translation initiation factor 4E binding protein 1 antibody,MGC4316 antibody, PHAS I antibody, PHASI antibody, Phosphorylated heat and acidstable protein regulated by insulin 1 antibody, eIF4E binding protein 1 antibody.
Gene Symbol: EIF4EBP1
|Pathways||MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling|
Application DetailsProduct Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Handling References for anti-EIF4EBP1 Antibody (ABIN189001) Images back to top
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 15-20kDa.
Recommended dilutions: Flow Cytometry 1:20, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50-100, Immunoprecipitation 1:50, Western Blot 1:1000-10000
Protocol specific for eIF4EBP1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details References for anti-EIF4EBP1 Antibody (ABIN189001) Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
References for anti-EIF4EBP1 Antibody (ABIN189001)Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling Images back to top
|Product cited in:||
Escobar, Frank, Suryawan et al.: "Amino acid availability and age affect the leucine stimulation of protein synthesis and eIF4F formation in muscle." in: American journal of physiology. Endocrinology and metabolism, Vol. 293, Issue 6, pp. E1615-21, 2007 (PubMed).
ImagesProduct Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN189001) back to top