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|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||55 Antibodies||151 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||4 Antibodies|
|Flow Cytometry (FACS)||12 Antibodies|
|Gel Shift (GS)||2 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||8 Antibodies|
|Immunofluorescence (IF)||15 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||57 Antibodies|
|Immunohistochemistry (IHC)||100 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||39 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Intracellular Staining (ICS)||4 Antibodies|
|Western Blotting (WB)||137 Antibodies|
|Dot Blot (DB)||18 Antibodies|
|Antigen||Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This EIF4EBP1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-EIF4EBP1 AntibodyTarget Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN499179) Images
|Specificity||4E-BP1 antibody was raised against a 14 amino acid peptide from near the carboxy-terminus of human 4E-BP1.|
|Purification||Affinity chromatography purified via peptide column|
|Immunogen||A 14 amino acid peptide from near the carboxy-terminus of Human 4E-BP1|
Target Details EIF4EBP1Product Details anti-EIF4EBP1 Antibody Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN499179) Images back to top
|Alternative Name||EIF4EBP1 / 4E-BP1 (EIF4EBP1 Antibody Abstract)|
|Background||The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms (reviewed in 1). One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 2). One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5'-UTRs (1). Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 3), at least in part by inhibiting the activity of TOR towards 4E-BP1.Synonyms: Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-1, PHAS-I, PHAS1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, eIF4E-binding protein 1|
|Pathways||MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling|
Application DetailsProduct Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Handling References for anti-EIF4EBP1 Antibody (ABIN499179) Images back to top
ELISA. Western Blot: 4E-BP1 antibody can be used for the detection of 4E-BP1 at 2.5 - 5 μg/mL. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details References for anti-EIF4EBP1 Antibody (ABIN499179) Images back to top
|Buffer||PBS containing 0.02 % Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
References for anti-EIF4EBP1 Antibody (ABIN499179)Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling Images back to top
Shamji, Nghiem, Schreiber: "Integration of growth factor and nutrient signaling: implications for cancer biology." in: Molecular cell, Vol. 12, Issue 2, pp. 271-80, 2003 (PubMed).
Shah, Anthony, Kimball et al.: "4E-BP1 and S6K1: translational integration sites for nutritional and hormonal information in muscle." in: American journal of physiology. Endocrinology and metabolism, Vol. 279, Issue 4, pp. E715-29, 2000 (PubMed).
ImagesProduct Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Handling References for anti-EIF4EBP1 Antibody (ABIN499179) back to top