anti-Rat (Rattus) EIF4EBP1 antibody for Flow Cytometry

Recommended EIF4EBP1 Antibody (supplied by: Log in to see )

Antigen
Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
  • 153432_at
  • 43-BP
  • 4E-BP
  • 4E-BP1
  • 4EBP
  • 4e-bp
  • 4eBP
  • 4ebp
  • BcDNA.HL08053
  • BcDNA:HL08053
  • CG8846
  • CT8705
  • Dm 4E-BP
  • Dmel\\CG8846
  • Phas1
  • Thor/EIF4EBP
  • anon-WO0172774.158
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4e-bp
  • d4eBP
  • dthor
  • eIF4E-BP
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • pp20
  • thor
  • EIF4EBP1
  • fc04h09
  • wu:fc04h09
  • zgc:64137
  • 4EBP1
  • BP-1
  • PHAS-I
  • 4e-bp1
  • AA959816
  • CG8846 gene product from transcript CG8846-RA
  • eukaryotic translation initiation factor 4E binding protein 1
  • Thor
  • EIF4EBP1
  • eif4ebp1
  • LOC100230789
  • Eif4ebp1
Epitope
pThr37, pThr46
62
36
30
27
25
22
19
16
15
15
15
15
15
12
12
8
8
7
6
5
5
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Fruit Fly (Drosophila melanogaster), Human, Monkey, Mouse (Murine), Rat (Rattus)
461
277
235
20
17
12
9
5
5
5
3
2
2
1
1
1
1
Host
Rabbit
401
97
4
Clonality
Monoclonal
Conjugate
This EIF4EBP1 antibody is un-conjugated
19
17
11
10
8
8
5
5
5
5
5
5
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
345
216
201
110
66
29
17
17
14
8
7
6
5
4
3
1
Supplier
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Catalog No. ABIN354772
$ 837.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN603974 FACS WB Biotin Rabbit IgG pThr37, pThr46 Log in to see
1 ABIN229451 FACS ELISA WB Rabbit IgG pThr46 Log in to see

General

Antigen Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) Antibodies
Epitope pThr37, pThr46
(62), (36), (30), (27), (25), (22), (19), (16), (15), (15), (15), (15), (15), (12), (12), (8), (8), (7), (6), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Fruit Fly (Drosophila melanogaster), Human, Monkey, Mouse (Murine), Rat (Rattus)
(461), (277), (235), (20), (17), (12), (9), (5), (5), (5), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(401), (97), (4)
Clonality
Monoclonal
Conjugate This EIF4EBP1 antibody is un-conjugated
(19), (17), (11), (10), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(345), (216), (201), (110), (66), (29), (17), (17), (14), (8), (7), (6), (5), (4), (3), (1)
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Product Details anti-EIF4EBP1 Antibody

Target Details EIF4EBP1 Application Details Handling Images
Specificity Recognizes endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. May cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Species cross-reactivity: human, mouse, rat, monkey, and D. melanogaster.
Purification Ascites
Immunogen Synthetic phospho-peptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1 (KLH-coupled).

Type of Immunogen: Synthetic phospho-peptide
Isotype IgG

Target Details EIF4EBP1

Product Details anti-EIF4EBP1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name EIF4EBP1 / 4EBP1 (EIF4EBP1 Antibody Abstract)
Background Name/Gene ID: EIF4EBP1

Synonyms: EIF4EBP1, 4E-BP1, 4EBP1, BP-1, PHAS-I, EIF4E-binding protein 1
Gene ID 1978
UniProt Q13541
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size

Application Details

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Handling Images back to top
Application Notes Approved: Flo (1:100), IF, IHC-P (1:400), WB (1:1000)

Usage: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and Flow cytometry. Western Blot: 1:1000. For Western blots, incubate membrane with diluted antibody in 5 % w/v BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:400 (Citrate/TBST). Immunofluorescence (IF-IC): 1:50. Flow Cytometry: 1:100.
Restrictions For Research Use only

Handling

Product Details anti-EIF4EBP1 Antibody Target Details EIF4EBP1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol, 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.