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anti-Human ELK1 antibody for Enzyme Immunoassay

Recommended ELK1 Antibody (supplied by: Log in to see )

Antigen
ELK1, Member of ETS Oncogene Family (ELK1) Antibodies
  • BEC2
  • Elk-1
  • ELK1
  • Kv12.3
Epitope
Ser383
75
33
33
25
12
8
7
5
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
350
174
168
11
4
4
2
2
2
2
1
Host
Rabbit
279
76
Clonality
Polyclonal
Conjugate
This ELK1 antibody is un-conjugated
7
7
7
6
6
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
240
139
127
41
41
34
33
6
4
3
2
1
1
Supplier
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Catalog No. ABIN358791
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN1107085 EIA IHC (p) WB Mouse IgG1 Log in to see 3H6D12 4
0.0008867729 ABIN1107086 EIA WB Mouse IgG1 Log in to see 7E10D5 4
0.0008867729 ABIN318012 EIA IHC (p) IP WB Rabbit pSer383 Log in to see Polyclonal
0.0008867729 ABIN452640 EIA Mouse IgG1 pSer383 Log in to see B4A12
0.0008867729 ABIN452642 EIA Mouse IgG2b pSer383 Log in to see D7H2
0.0008867729 ABIN2720129 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-ELK1 antibody for Enzyme Immunoassay

Similar anti-ELK1 Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 127 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 34 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 140 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies
Immunoprecipitation (IP) 42 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 235 Antibodies

General

Antigen ELK1, Member of ETS Oncogene Family (ELK1) Antibodies
Epitope Ser383
(75), (33), (33), (25), (12), (8), (7), (5), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(350), (174), (168), (11), (4), (4), (2), (2), (2), (2), (1)
Host Rabbit
(279), (76)
Clonality
Polyclonal
Conjugate This ELK1 antibody is un-conjugated
(7), (7), (7), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(240), (139), (127), (41), (41), (34), (33), (6), (4), (3), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-ELK1 Antibody

Target Details ELK1 Application Details Handling References for anti-ELK1 Antibody (ABIN358791) Images
Specificity This antibody reacts to ELK1.
Purification Protein A column, followed by peptide affinity purification
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S383 of human ELK1.
Isotype Ig

Target Details ELK1

Product Details anti-ELK1 Antibody Application Details Handling References for anti-ELK1 Antibody (ABIN358791) Images back to top
Antigen
Alternative Name ELK1 (ELK1 Antibody Abstract)
Background ELK1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein is a nuclear target for the ras-raf-MAPK signaling cascade.Synonyms: ETS domain-containing protein Elk-1, Elk-1
Gene ID 2002, 9606
UniProt P19419
Research Area Cancer, Transcription Factors
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-ELK1 Antibody Target Details ELK1 Handling References for anti-ELK1 Antibody (ABIN358791) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-ELK1 Antibody Target Details ELK1 Application Details References for anti-ELK1 Antibody (ABIN358791) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-ELK1 Antibody (ABIN358791)

Product Details anti-ELK1 Antibody Target Details ELK1 Application Details Handling Images back to top
Background publications

Salinas, Briançon-Marjollet, Bossis, Lopez, Piechaczyk, Jariel-Encontre, Debant, Hipskind: "SUMOylation regulates nucleo-cytoplasmic shuttling of Elk-1." in: The Journal of cell biology, Vol. 165, Issue 6, pp. 767-73, 2004

Yang, Jaffray, Hay, Sharrocks: "Dynamic interplay of the SUMO and ERK pathways in regulating Elk-1 transcriptional activity." in: Molecular cell, Vol. 12, Issue 1, pp. 63-74, 2003

Bébien, Salinas, Becamel, Richard, Linares, Hipskind: "Immediate-early gene induction by the stresses anisomycin and arsenite in human osteosarcoma cells involves MAPK cascade signaling to Elk-1, CREB and SRF." in: Oncogene, Vol. 22, Issue 12, pp. 1836-47, 2003

Murai, Treisman: "Interaction of serum response factor (SRF) with the Elk-1 B box inhibits RhoA-actin signaling to SRF and potentiates transcriptional activation by Elk-1." in: Molecular and cellular biology, Vol. 22, Issue 20, pp. 7083-92, 2002

Sharrocks: "Complexities in ETS-domain transcription factor function and regulation: lessons from the TCF (ternary complex factor) subfamily. The Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 30, Issue 2, pp. 1-9, 2002

Images

Product Details anti-ELK1 Antibody Target Details ELK1 Application Details Handling References for anti-ELK1 Antibody (ABIN358791) back to top
Supplier Images
 image for anti-ELK1 antibody (ELK1, Member of ETS Oncogene Family) (Ser383) (ABIN358791) anti-ELK1, Member of ETS Oncogene Family (ELK1) (Ser383) antibody