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anti-Mouse (Murine) ELK1 antibody for Immunocytochemistry

Recommended ELK1 Antibody (supplied by: Log in to see )

Antigen
ELK1, Member of ETS Oncogene Family (ELK1) Antibodies
  • BEC2
  • Elk-1
  • ELK1
  • Kv12.3
Epitope
C-Term
73
33
33
26
12
8
7
5
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
348
173
166
11
4
4
2
2
2
2
1
Host
Rabbit
278
74
Clonality
Monoclonal
Conjugate
This ELK1 antibody is un-conjugated
7
7
7
6
6
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
237
136
125
41
40
34
33
7
4
3
2
1
1
Supplier
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Available images

Catalog No. ABIN189327
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010124836 ABIN2987199 ICC IF IHC WB Rabbit IgG pSer383 Log in to see Polyclonal
0.0010124836 ABIN2988000 ICC IF IHC Rabbit IgG pThr417 Log in to see Polyclonal

Similar anti-ELK1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Chromatin Immunoprecipitation (ChIP) 1 Antibodies 1 Antibodies
Dot Blot (DB) 1 Antibodies 1 Antibodies
ELISA 27 Antibodies 30 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 3 Antibodies
Immunofluorescence (IF) 19 Antibodies 21 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies 34 Antibodies
Immunohistochemistry (IHC) 78 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies
Immunoprecipitation (IP) 29 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 94 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies

General

Antigen ELK1, Member of ETS Oncogene Family (ELK1) Antibodies
Epitope C-Term
(73), (33), (33), (26), (12), (8), (7), (5), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(348), (173), (166), (11), (4), (4), (2), (2), (2), (2), (1)
Host Rabbit
(278), (74)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ELK1 antibody is un-conjugated
(7), (7), (7), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(237), (136), (125), (41), (40), (34), (33), (7), (4), (3), (2), (1), (1)
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Product Details anti-ELK1 Antibody

Target Details ELK1 Application Details Handling Images
Specificity The antibody does not cross-react with other ETS family members.
Cross-Reactivity (Details) Cross reactivity determined by Western Blot only.
Purification Protein A purified
Immunogen A synthetic peptide corresponding to residues in C-terminus of human ELK-1 was used as immunogen.
Clone Y77
Isotype IgG

Target Details ELK1

Product Details anti-ELK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ELK1 (ELK1 Antibody Abstract)
Background Elk-1 is a transcription factor that is part of the ets oncogene superfamily (1). Elk-1forms a ternary complex with the serum response factors, SRE and SRF, mediatinggene activity in response to serum and growth factors (2). Activation by severalmitogen-activated protein kinases (Erk1 and Erk2) mediates the immediate earlyresponses of the c-fos promoter to growth factors and other stimuli (3,4). Serines 383and 389 in the C-terminal region are the preferred sites for phosphorylation-inducedactivation. Alternate Names: anti-ELK 1 antibody, anti-ELK1 member of ETS oncogene family antibody, anti-ELK1protein antibody, anti-ELK2 member of ETS oncogene family antibody, anti-ETS domaincontaining protein Elk 1 antibody, anti-ETS domain containing protein Elk1 antibody,anti-ETS domain protein Elk1 antibody, anti-Oncogene Elk1 antibody.
Gene Symbol: ELK1
Gene ID 2002, 13712, 314436
Research Area Cancer, Transcription Factors
Pathways MAPK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-ELK1 Antibody Target Details ELK1 Handling Images back to top
Application Notes In Western blot this antibody detects a band at approximately 62 kDa. Chromatin Immunoprecipitation was reported in scientific literature.
Recommended dilutions: Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry 1:50, Immunohistochemistry-Paraffin 1:50, Immunoprecipitation 1:80, Western Blot 1:500
Protocol Protocol specific for ELK1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-ELK1 Antibody Target Details ELK1 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-ELK1 Antibody Target Details ELK1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-ELK1 antibody (ELK1, Member of ETS Oncogene Family) (C-Term) (ABIN189327) Western blot analysis on cell lysate using anti-Elk-1 (C-term) RabMAb (catalog #110-5...