|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Chromatin Immunoprecipitation (ChIP)||1 Antibodies||1 Antibodies|
|Dot Blot (DB)||1 Antibodies||1 Antibodies|
|ELISA||27 Antibodies||30 Antibodies|
|Immunochromatography (IC)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||3 Antibodies|
|Immunofluorescence (IF)||19 Antibodies||21 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||34 Antibodies||34 Antibodies|
|Immunohistochemistry (IHC)||78 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||25 Antibodies|
|Immunoprecipitation (IP)||29 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies|
|Western Blotting (WB)||94 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Antigen||ELK1, Member of ETS Oncogene Family (ELK1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This ELK1 antibody is un-conjugated Alternatives|
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-ELK1 AntibodyTarget Details ELK1 Application Details Handling Images
|Specificity||The antibody does not cross-react with other ETS family members.|
|Cross-Reactivity (Details)||Cross reactivity determined by Western Blot only.|
|Purification||Protein A purified|
|Immunogen||A synthetic peptide corresponding to residues in C-terminus of human ELK-1 was used as immunogen.|
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|Alternative Name||ELK1 (ELK1 Antibody Abstract)|
Elk-1 is a transcription factor that is part of the ets oncogene superfamily (1). Elk-1forms a ternary complex with the serum response factors, SRE and SRF, mediatinggene activity in response to serum and growth factors (2). Activation by severalmitogen-activated protein kinases (Erk1 and Erk2) mediates the immediate earlyresponses of the c-fos promoter to growth factors and other stimuli (3,4). Serines 383and 389 in the C-terminal region are the preferred sites for phosphorylation-inducedactivation. Alternate Names: anti-ELK 1 antibody, anti-ELK1 member of ETS oncogene family antibody, anti-ELK1protein antibody, anti-ELK2 member of ETS oncogene family antibody, anti-ETS domaincontaining protein Elk 1 antibody, anti-ETS domain containing protein Elk1 antibody,anti-ETS domain protein Elk1 antibody, anti-Oncogene Elk1 antibody.
Gene Symbol: ELK1
|Gene ID||2002, 13712, 314436|
|Research Area||Cancer, Transcription Factors|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-ELK1 Antibody Target Details ELK1 Handling Images back to top
In Western blot this antibody detects a band at approximately 62 kDa. Chromatin Immunoprecipitation was reported in scientific literature.
Recommended dilutions: Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry 1:50, Immunohistochemistry-Paraffin 1:50, Immunoprecipitation 1:80, Western Blot 1:500
Protocol specific for ELK1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-ELK1 Antibody Target Details ELK1 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
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