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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Dot Blot (DB)||1 Antibodies||1 Antibodies|
|ELISA||28 Antibodies||33 Antibodies|
|Immunochromatography (IC)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||1 Antibodies|
|Immunofluorescence (IF)||20 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||3 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||40 Antibodies|
|Immunohistochemistry (IHC)||85 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||34 Antibodies|
|Immunoprecipitation (IP)||35 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Western Blotting (WB)||110 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Antigen||ELK1, Member of ETS Oncogene Family (ELK1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This ELK1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-ELK1 AntibodyTarget Details ELK1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of ELK1 protein (region surrounding Ser383).|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details ELK1Product Details anti-ELK1 Antibody Application Details Handling Images back to top
|Alternative Name||ELK1 (ELK1 Antibody Abstract)|
|Background||Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1-3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5'-GGAA-3' element.Synonyms: ETS domain-containing protein Elk-1, Elk-1|
|Molecular Weight||approx. 45 kDa|
|Research Area||Cancer, Transcription Factors|
|Pathways||MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-ELK1 Antibody Target Details ELK1 Handling Images back to top
ELISA: 1: 20000approx. 1: 40000. IHC: 1: 50approx. 1: 200. IP: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ELK1 Antibody Target Details ELK1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-ELK1 Antibody Target Details ELK1 Application Details Handling back to top