anti-Human ERK2 antibody for Immunofluorescence

Recommended ERK2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
  • ATMPK1
  • F14N23.9
  • F14N23_9
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • ATMPK2
  • MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2
  • T30E16.13
  • T30E16_13
  • mitogen-activated protein kinase homolog 2
  • ERK2
  • zERK2
  • MAPK1
  • ERK
  • ERT1
  • MAPK2
  • P42MAPK
  • PRKM1
  • PRKM2
  • p38
  • p40
  • p41
  • p41mapk
  • 9030612K14Rik
  • AA407128
  • AU018647
  • C78273
  • Erk2
  • Prkm1
  • p42mapk
  • erk
  • erk2
  • ert1
  • mapk
  • mpk1
  • xp42
  • mapk2
  • prkm1
  • prkm2
  • Xp42
  • mapk1b
  • mapk1
  • mapk1a
  • mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 2
  • mitogen activated protein kinase 1
  • ATMPK1
  • MPK1
  • MPK2
  • MAPK1
  • mapk1
  • Mapk1
  • PfMAP1
  • PVX_084965
  • Tsp_01601
  • mapk1-b
  • mapk1-a
Epitope
AA 219-358
60
22
16
11
9
9
8
8
5
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
351
147
118
50
30
19
14
10
10
9
8
7
5
5
2
2
2
2
1
1
1
1
Host
Mouse
167
167
17
8
3
Clonality
Monoclonal
Conjugate
This ERK2 antibody is un-conjugated
10
9
8
7
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
324
157
92
81
81
61
50
26
21
10
5
3
3
2
2
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN967747
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.326117 ABIN967748 IF IP IHC WB Mouse IgG2b AA 219-358 Log in to see 33-ERK2 5
12.326117 ABIN1107133 IF IHC (p) WB Mouse IgG2a Log in to see 4C11 2
12.326117 ABIN492382 EIA IF WB Mouse IgG2b AA 1-360, N-Term Log in to see AT1A4
12.326117 ABIN258758 ICC IHC (fro) IF IHC (p) IHC ELISA WB Mouse IgG2a Log in to see 4C11
12.326117 ABIN2452333 IF IP WB Mouse IgG1 Log in to see 7E7
12.326117 ABIN2452330 IF IP WB Mouse IgG2a Log in to see 7C9
12.326117 ABIN1873622 IF IHC WB Rabbit IgG N-Term Log in to see Polyclonal
12.326117 ABIN4309415 ICC IF IHC IHC (p) SimWes WB Rabbit IgG Log in to see Polyclonal
12.326117 ABIN2475489 IF WB Mouse IgG1 AA 261-361 Log in to see 1D1
12.326117 ABIN3210001 IF IHC ELISA WB Mouse IgG2a Log in to see 4C11
12.326117 ABIN562405 IF PLA ELISA WB Mouse IgG1 kappa AA 261-360, full length Log in to see 1D1
12.326117 ABIN934060 IF ELISA WB Mouse IgG2b kappa Log in to see AT1A4
1 ABIN393485 IF WB Mouse IgG1 kappa AA 261-361 Log in to see 1D1 5
1 ABIN3184547 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN2452608 IF IP IHC WB Mouse IgG1 Log in to see 4C2
1 ABIN1872585 IF IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN1499288 IF WB Mouse IgG2a Log in to see 7C9
1 ABIN1499290 IF IP WB Mouse IgG1 Log in to see 7E7
1 ABIN1499289 IF IHC (p) IP WB Mouse IgG1 Log in to see 4C2
1 ABIN438932 ICC IF IHC IHC (p) IP PLA WB Rabbit AA 310-360 Log in to see Polyclonal

Top referenced anti-ERK2 antibody for Immunofluorescence

Similar anti-ERK2 Antibodies

Application / Reactivity Human Mouse (Murine) Dog (Canine) Rat (Rattus) Chicken Frog
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 1 Antibodies
Simple Western (SimWes) 2 Antibodies
Western Blotting (WB) 314 Antibodies 30 Antibodies 135 Antibodies 112 Antibodies 9 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies 2 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Luminex Assay (LMNX) 5 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
Epitope AA 219-358
(60), (22), (16), (11), (9), (9), (8), (8), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
(351), (147), (118), (50), (30), (19), (14), (10), (10), (9), (8), (7), (5), (5), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(167), (167), (17), (8), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ERK2 antibody is un-conjugated
(10), (9), (8), (7), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(324), (157), (92), (81), (81), (61), (50), (26), (21), (10), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-ERK2 Antibody

Target Details ERK2 Application Details Handling ProductDetails: References for anti-ERK2 antibody (ABIN967747) Images
Cross-Reactivity Human, Dog (Canine), Mouse (Murine), Chicken, Frog
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat ERK2 aa. 219-358
Clone 33-ERK2
Isotype IgG2b

Target Details ERK2

Product Details anti-ERK2 Antibody Application Details Handling ProductDetails: References for anti-ERK2 antibody (ABIN967747) Images back to top
Antigen
Alternative Name ERK2 (MAPK1 Antibody Abstract)
Background The family of serine/threonine kinases known as ERKs (extracellular signal regulated kinases) or MAPKs (mitogen-activated protein kinases) are activated after cell stimulation by a wide variety of hormones and growth factors. Cell stimulation induces a signaling cascade that leads to phosphorylation of MEK (MAPK/ERK kinase) which, in turn, activates ERK via tyrosine and threonine phosphorylation. Structural analysis of ERK2 indicates that phosphorylation induces a conformational change that exposes the active site for substrate binding. Myriad proteins represent the downstream effectors for the active ERK and implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Activation of ERK is normally transient and cells possess dual specificity phosphatases that are responsible for its down-regulation. Furthermore, multiple studies have shown that elevated ERK activity is associated with some cancers. ERK2 is the 42kDa member of the ERK family and is highly homologous to ERK1.
Molecular Weight 42 kDa
Research Area Stem Cells, Signaling, Inflammation
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Application Details

Product Details anti-ERK2 Antibody Target Details ERK2 Handling ProductDetails: References for anti-ERK2 antibody (ABIN967747) Images back to top
Comment

Related Products: ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details ProductDetails: References for anti-ERK2 antibody (ABIN967747) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

ProductDetails: References for anti-ERK2 antibody (ABIN967747)

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling Images back to top
Product cited in:

Kim, Ju, Oh, Yoon, Song, Kim, Yoo, Bang, Kang, Chun: "ERK-1/2 and p38 kinase oppositely regulate nitric oxide-induced apoptosis of chondrocytes in association with p53, caspase-3, and differentiation status." in: The Journal of biological chemistry, Vol. 277, Issue 2, pp. 1332-9, 2002

Lund-Johansen, Davis, Bishop, de Waal Malefyt: "Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions." in: Cytometry, Vol. 39, Issue 4, pp. 250-9, 2000

Lehmann, Janda, Pierreux, Rytömaa, Schulze, McMahon, Hill, Beug, Downward: "Raf induces TGFbeta production while blocking its apoptotic but not invasive responses: a mechanism leading to increased malignancy in epithelial cells." in: Genes & development, Vol. 14, Issue 20, pp. 2610-22, 2000

Liu, Tsai, Aird: "Egr-1 gene is induced by the systemic administration of the vascular endothelial growth factor and the epidermal growth factor." in: Blood, Vol. 96, Issue 5, pp. 1772-81, 2000

Visconti, Gadina, Chiariello, Chen, Stancato, Gutkind, OShea: "Importance of the MKK6/p38 pathway for interleukin-12-induced STAT4 serine phosphorylation and transcriptional activity." in: Blood, Vol. 96, Issue 5, pp. 1844-52, 2000

Images

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling ProductDetails: References for anti-ERK2 antibody (ABIN967747) back to top
Supplier Images
Western Blotting (WB) image for anti-ERK2 antibody (Mitogen-Activated Protein Kinase 1) (AA 219-358) (ABIN967747) Western blot analysis of ERK2 on rat pituitary lysate. Lane 1: 1:5000, lane 2: 1:1000...
Immunofluorescence (IF) image for anti-ERK2 antibody (Mitogen-Activated Protein Kinase 1) (AA 219-358) (ABIN967747) Immunofluorescence staining of MCF7 cells.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ERK2 antibody (Mitogen-Activated Protein Kinase 1) (AA 219-358) (ABIN967747) ERK 2 (clone 33) staining on rat brain. Formalin fixed paraffin section with citrate ...