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anti-Human ERK2 antibody for Immunoprecipitation

Recommended ERK2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
  • 9030612K14Rik
  • AA407128
  • ATMPK1
  • ATMPK2
  • AU018647
  • C78273
  • erk
  • ERK
  • ERK2
  • Erk2
  • erk2
  • ert1
  • ERT1
  • F14N23.9
  • F14N23_9
  • mapk
  • mapk1
  • MAPK1
  • mapk1a
  • mapk1b
  • MAPK2
  • mapk2
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2
  • mitogen-activated protein kinase homolog 2
  • mpk1
  • p38
  • p40
  • p41
  • p41mapk
  • p42mapk
  • P42MAPK
  • Prkm1
  • prkm1
  • PRKM1
  • PRKM2
  • prkm2
  • T30E16.13
  • T30E16_13
  • Xp42
  • xp42
  • zERK2
Epitope
C-Term
60
25
15
9
8
8
7
5
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
256
78
65
16
14
7
5
3
3
3
2
2
2
1
1
1
1
Host
Rabbit
152
107
16
3
1
Clonality
Polyclonal
Conjugate
This ERK2 antibody is un-conjugated
6
5
5
5
4
4
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
240
106
79
48
42
42
16
13
9
7
5
3
1
1
1
1
1
1
Supplier
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Catalog No. ABIN400970
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089235755 ABIN967397 IP WB Mouse IgG1, kappa Log in to see G263-7 1
0.00089235755 ABIN967747 IF IP IHC WB Mouse IgG2b AA 219-358 Log in to see 33-ERK2 5
0.00089235755 ABIN967748 IF IP IHC WB Mouse IgG2b AA 219-358 Log in to see 33-ERK2 5
0.00089235755 ABIN126832 EIA IP WB Mouse IgG1 C-Term Log in to see 6G11
0.00089235755 ABIN302975 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.00089235755 ABIN492351 EIA IP WB Biotin Mouse IgG1 C-Term Log in to see 6G11
0.00089235755 ABIN1499290 IF IP WB Mouse IgG1 Log in to see 7E7
0.00089235755 ABIN1499291 IHC (p) IP WB Mouse IgG1 Log in to see 9E9
0.00089235755 ABIN1499289 IF IHC (p) IP WB Mouse IgG1 Log in to see 4C2
0.00089235755 ABIN2452284 IP WB Mouse IgG1 Log in to see 1D3
0.00089235755 ABIN2452285 IP WB Mouse IgG1 Log in to see 1C11
0.00089235755 ABIN2452283 IP WB Mouse IgG2a Log in to see 4D10
0.00089235755 ABIN2452330 IF IP WB Mouse IgG2a Log in to see 7C9
0.00089235755 ABIN2452333 IF IP WB Mouse IgG1 Log in to see 7E7
0.00089235755 ABIN2452387 IP IHC WB Mouse IgG1 Log in to see 9E9
0.00089235755 ABIN2452462 IP IHC WB Mouse IgG1 Log in to see 6E5
0.00089235755 ABIN2452463 IP IHC WB Mouse IgG2a Log in to see 9H1
0.00089235755 ABIN2452608 IF IP IHC WB Mouse IgG1 Log in to see 4C2
0.00089235755 ABIN2452552 FACS IP IHC WB Mouse IgG2a Log in to see 6F8
0.00089235755 ABIN342484 IP ELISA WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-ERK2 antibody for Immunoprecipitation

Similar anti-ERK2 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Dot Blot (DB) 2 Antibodies
ELISA 100 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunofluorescence (IF) 44 Antibodies
Immunohistochemistry (IHC) 74 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies
Immunoprecipitation (IP) 38 Antibodies
Immunostaining (ISt) 1 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Luminex Assay (LMNX) 5 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 222 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
Epitope C-Term
(60), (25), (15), (9), (8), (8), (7), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(256), (78), (65), (16), (14), (7), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(152), (107), (16), (3), (1)
Clonality
Polyclonal
Conjugate This ERK2 antibody is un-conjugated
(6), (5), (5), (5), (4), (4), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(240), (106), (79), (48), (42), (42), (16), (13), (9), (7), (5), (3), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-ERK2 Antibody

Target Details ERK2 Application Details Handling References for anti-ERK2 Antibody (ABIN400970) Images
Specificity This antibody reacts to p42 MAP Kinase (ERK2) protein. No reactivity is observed against p44 MAP Kinase (ERK1). Cross reactivity is expected with p44 MAP Kinase (ERK1) proteins from human and mouse sources.
Cross-Reactivity (Details) Species reactivity (expected):Rat.
Species reactivity (tested):Human.
Purification Serum
Immunogen This whole rabbit antiserum was prepared by repeated immunizations with a GST fusion protein. The epitope maps near the carboxy-terminus of human p42 MAP Kinase (ERK2) protein. The epitope is identical to the corresponding sequence in mouse and differs from the rat sequence by a single, conservative amino acid substitution.
Isotype IgG

Target Details ERK2

Product Details anti-ERK2 Antibody Application Details Handling References for anti-ERK2 Antibody (ABIN400970) Images back to top
Antigen
Alternative Name ERK2 (MAPK1 Antibody Abstract)
Background Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses. In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPK's) ERK1 and ERK2 of 44 kDa and 42 kDa, respectively. When activated, ERK's form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the upregulation of immediate early genes. As such, ERK1 and ERK2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells. These enzymes function within highly conserved cascade of sequentially activating protein kinases that transduce signals from diverse extracellular stimuli. Alternative splice transcript variants encoding different protein isoforms have been described. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2. In vertebrates the mitogeninduced sequential activation of the kinases Raf1->Mek1->Erk2->Rsk occurs via the G-protein Ras.Synonyms: ERT1, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, PRKM1, PRKM2, p42-MAPK
Gene ID 5594
NCBI Accession NP_002736
UniProt P28482
Research Area Stem Cells, Signaling, Inflammation
Pathways MAPK Signaling, RTK Signaling, Apoptosis, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-ERK2 Antibody Target Details ERK2 Handling References for anti-ERK2 Antibody (ABIN400970) Images back to top
Application Notes ELISA: 1/2000 to 1/10000. Western Blot: 1/1000-1/2000. Immunopreicpitation: 5.0 μg/mg sample.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details References for anti-ERK2 Antibody (ABIN400970) Images back to top
Format Liquid
Concentration 85 mg/mL (by Refractometry)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-ERK2 Antibody (ABIN400970)

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling Images back to top
Background publications

Asthagiri, Reinhart, Horwitz, Lauffenburger: "The role of transient ERK2 signals in fibronectin- and insulin-mediated DNA synthesis." in: Journal of cell science, Vol. 113 Pt 24, pp. 4499-510, 2001

Hoffmann, Baillie, MacKenzie, Yarwood, Houslay: "The MAP kinase ERK2 inhibits the cyclic AMP-specific phosphodiesterase HSPDE4D3 by phosphorylating it at Ser579." in: The EMBO journal, Vol. 18, Issue 4, pp. 893-903, 1999

Garrington, Johnson: "Organization and regulation of mitogen-activated protein kinase signaling pathways." in: Current opinion in cell biology, Vol. 11, Issue 2, pp. 211-8, 1999

Elion: "Routing MAP kinase cascades." in: Science (New York, N.Y.), Vol. 281, Issue 5383, pp. 1625-6, 1998

Pelech, Charest: "MAP kinase-dependent pathways in cell cycle control." in: Progress in cell cycle research, Vol. 1, pp. 33-52, 1998

Images

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling References for anti-ERK2 Antibody (ABIN400970) back to top
Supplier Images
 image for anti-ERK2 antibody (Mitogen-Activated Protein Kinase 1) (C-Term) (ABIN400970) anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (C-Term) antibody