anti-Human ERK2 antibody for Western Blotting

Recommended ERK2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
  • ATMPK1
  • F14N23.9
  • F14N23_9
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • ATMPK2
  • MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2
  • T30E16.13
  • T30E16_13
  • mitogen-activated protein kinase homolog 2
  • ERK2
  • zERK2
  • MAPK1
  • ERK
  • ERT1
  • MAPK2
  • P42MAPK
  • PRKM1
  • PRKM2
  • p38
  • p40
  • p41
  • p41mapk
  • 9030612K14Rik
  • AA407128
  • AU018647
  • C78273
  • Erk2
  • Prkm1
  • p42mapk
  • erk
  • erk2
  • ert1
  • mapk
  • mpk1
  • xp42
  • mapk2
  • prkm1
  • prkm2
  • Xp42
  • mapk1b
  • mapk1
  • mapk1a
  • mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 2
  • mitogen activated protein kinase 1
  • ATMPK1
  • MPK1
  • MPK2
  • MAPK1
  • mapk1
  • Mapk1
  • PfMAP1
  • PVX_084965
  • Tsp_01601
  • mapk1-b
  • mapk1-a
Epitope
N-Term
60
22
15
11
9
9
8
8
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
351
147
118
50
30
19
14
11
10
9
8
7
5
5
2
2
2
2
2
1
1
1
Host
Mouse
167
167
17
8
3
Clonality
Monoclonal
Conjugate
This ERK2 antibody is un-conjugated
10
9
8
7
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
324
157
93
82
81
62
50
26
21
9
5
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN126833
$ 478.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3484275 ABIN391367 FACS IHC (p) WB Rabbit Ig AA 154-183, Center Log in to see Polyclonal 3
3.3484275 ABIN391720 IHC (p) WB Rabbit Ig AA 254-285, C-Term Log in to see Polyclonal 2
3.3484275 ABIN1882240 WB Mouse IgG1, kappa AA 154-183 Log in to see 814CT5-4-2 5
3.3484275 ABIN268704 ELISA WB Goat AA 9-19 Log in to see Polyclonal 1
3.3484275 ABIN393485 IF WB Mouse IgG1 kappa AA 261-361 Log in to see 1D1 5
3.3484275 ABIN126832 EIA IP WB Mouse IgG1 C-Term Log in to see 6G11
3.3484275 ABIN967747 IF IP IHC WB Mouse IgG2b AA 219-358 Log in to see 33-ERK2 5
3.3484275 ABIN967397 IP WB Mouse IgG1, kappa Log in to see G263-7 1
3.3484275 ABIN967748 IF IP IHC WB Mouse IgG2b AA 219-358 Log in to see 33-ERK2 5
3.3484275 ABIN1882175 FACS IHC (p) WB Rabbit AA 316-345, C-Term Log in to see Polyclonal 5
3.3484275 ABIN1724654 ICC IHC ELISA WB Mouse IgG2a Log in to see 4C11 2
3.3484275 ABIN2787870 WB Rabbit C-Term Log in to see Polyclonal
3.3484275 ABIN2787871 WB Rabbit Middle Region Log in to see Polyclonal
3.3484275 ABIN2788693 WB Rabbit C-Term Log in to see Polyclonal
3.3484275 ABIN1107133 IF IHC (p) WB Mouse IgG2a Log in to see 4C11 2
3.3484275 ABIN492382 EIA IF WB Mouse IgG2b AA 1-360, N-Term Log in to see AT1A4
3.3484275 ABIN3044305 IHC (p) WB Rabbit IgG AA 171-184, N-Term Log in to see Polyclonal
3.3484275 ABIN3042634 WB Rabbit IgG AA 348-360, C-Term Log in to see Polyclonal 2
3.3484275 ABIN2855862 WB Rabbit IgG N-Term Log in to see Polyclonal
3.3484275 ABIN2465663 ELISA WB Goat Internal Region Log in to see Polyclonal

Top referenced anti-ERK2 antibody for Western Blotting

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General

Antigen Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
Epitope N-Term
(60), (22), (15), (11), (9), (9), (8), (8), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(351), (147), (118), (50), (30), (19), (14), (11), (10), (9), (8), (7), (5), (5), (2), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(167), (167), (17), (8), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ERK2 antibody is un-conjugated
(10), (9), (8), (7), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(324), (157), (93), (82), (81), (62), (50), (26), (21), (9), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-ERK2 Antibody

Target Details ERK2 Application Details Handling ProductDetails: References for anti-ERK2 antibody (ABIN126833) Images
Specificity This antibody specifically recognizes the N-terminus of MAP kinase 2 (ERK2).
Characteristics Synonyms: Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2,MAPK2, MAPK1, Extracellular signal-regulated kinase 2
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Components incl. positive Control
Immunogen Peptide conjugated to KLHEpitope: N-terminus
Clone 6H3
Isotype IgG1

Target Details ERK2

Product Details anti-ERK2 Antibody Application Details Handling ProductDetails: References for anti-ERK2 antibody (ABIN126833) Images back to top
Antigen
Alternative Name ERK2 (MAPK1 Antibody Abstract)
Background Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.Synonyms: ERT1, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, PRKM1, PRKM2, p42-MAPK
Molecular Weight 42 kDa
Gene ID 5594
UniProt P28482
Research Area Stem Cells, Signaling, Inflammation
Pathways MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Application Details

Product Details anti-ERK2 Antibody Target Details ERK2 Handling ProductDetails: References for anti-ERK2 antibody (ABIN126833) Images back to top
Application Notes ELISA: Use at 0.05 μg/mL. Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated HepG2 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive Control Provided. Cell lysate from untreated HepG2Description: Cell lysate from untreated HepG2 cells, hepatocellular carcinoma (human)Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (minigel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.
Restrictions For Research Use only

Handling

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details ProductDetails: References for anti-ERK2 antibody (ABIN126833) Images back to top
Reconstitution Restore with 1.0 mL H2O (15 min, RT).
Buffer 1 mL2 x PBS containing PEG and Sucrose, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Expiry Date 12 months

ProductDetails: References for anti-ERK2 antibody (ABIN126833)

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling Images back to top
Product cited in:

Samuels, Karlo, Faruzzi, Pickering, Herrup, Sweatt, Saitta, Landreth: "Deletion of ERK2 mitogen-activated protein kinase identifies its key roles in cortical neurogenesis and cognitive function." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 27, pp. 6983-95, 2008

Images

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling ProductDetails: References for anti-ERK2 antibody (ABIN126833) back to top
Supplier Images
Western Blotting (WB) image for anti-ERK2 antibody (Mitogen-Activated Protein Kinase 1) (N-Term) (ABIN126833) anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (N-Term) antibody