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anti-Human ERK2 antibody for Western Blotting

Recommended ERK2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
  • 9030612K14Rik
  • AA407128
  • ATMPK1
  • ATMPK2
  • AU018647
  • C78273
  • erk
  • ERK
  • ERK2
  • Erk2
  • erk2
  • ert1
  • ERT1
  • F14N23.9
  • F14N23_9
  • mapk
  • mapk1
  • MAPK1
  • mapk1a
  • mapk1b
  • MAPK2
  • mapk2
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2
  • mitogen-activated protein kinase homolog 2
  • mpk1
  • p38
  • p40
  • p41
  • p41mapk
  • p42mapk
  • P42MAPK
  • Prkm1
  • prkm1
  • PRKM1
  • PRKM2
  • prkm2
  • T30E16.13
  • T30E16_13
  • Xp42
  • xp42
  • zERK2
Epitope
N-Term
62
25
14
9
8
8
7
5
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
314
98
68
33
16
7
5
3
3
3
2
2
2
1
1
1
1
Host
Mouse
160
157
16
3
2
Clonality
Monoclonal
Conjugate
This ERK2 antibody is un-conjugated
8
8
7
7
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
298
140
83
78
65
51
49
25
17
9
5
3
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN126833
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13357732 ABIN359461 EIA IHC (p) WB Rabbit Ig Middle Region Log in to see Polyclonal 5
0.13357732 ABIN359631 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 5
0.13357732 ABIN391367 FACS IHC (p) WB Rabbit Ig AA 154-183, Center Log in to see Polyclonal 3
0.13357732 ABIN391720 IHC (p) WB Rabbit Ig AA 254-285, C-Term Log in to see Polyclonal 2
0.13357732 ABIN1882240 WB Mouse IgG1, kappa AA 154-183 Log in to see 814CT5-4-2 5
0.13357732 ABIN393485 IF WB Mouse IgG1 kappa AA 261-361 Log in to see 1D1 5
0.13357732 ABIN1724654 ICC IHC ELISA WB Mouse IgG2a Log in to see 4C11 2
0.13357732 ABIN967397 IP WB Mouse IgG1, kappa Log in to see G263-7 1
0.13357732 ABIN967747 IF IP IHC WB Mouse IgG2b AA 219-358 Log in to see 33-ERK2 5
0.13357732 ABIN967748 IF IP IHC WB Mouse IgG2b AA 219-358 Log in to see 33-ERK2 5
0.13357732 ABIN126832 EIA IP WB Mouse IgG1 C-Term Log in to see 6G11
0.13357732 ABIN1882175 FACS IHC (p) WB Rabbit AA 316-345, C-Term Log in to see Polyclonal 5
0.13357732 ABIN1107133 IF IHC (p) WB Mouse IgG2a Log in to see 4C11 2
0.13357732 ABIN2787870 WB Rabbit C-Term Log in to see Polyclonal
0.13357732 ABIN2787871 WB Rabbit Middle Region Log in to see Polyclonal
0.13357732 ABIN492382 EIA IF WB Mouse IgG2b AA 1-360, N-Term Log in to see AT1A4
0.13357732 ABIN2855862 WB Rabbit IgG N-Term Log in to see Polyclonal
0.13357732 ABIN2465663 ELISA WB Goat Internal Region Log in to see Polyclonal
0.13357732 ABIN1533683 ELISA WB Rabbit IgG Log in to see Polyclonal
0.13357732 ABIN2706125 WB Rabbit Center Log in to see Polyclonal

Top referenced anti-ERK2 antibody for Western Blotting

Similar anti-ERK2 Antibodies

Application / Reactivity Human Dog (Canine) Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 279 Antibodies 17 Antibodies 89 Antibodies 64 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Luminex Assay (LMNX) 5 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 13 Antibodies 39 Antibodies 18 Antibodies 22 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies 65 Antibodies 10 Antibodies 1 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
Epitope N-Term
(62), (25), (14), (9), (8), (8), (7), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(314), (98), (68), (33), (16), (7), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(160), (157), (16), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ERK2 antibody is un-conjugated
(8), (8), (7), (7), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(298), (140), (83), (78), (65), (51), (49), (25), (17), (9), (5), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-ERK2 Antibody

Target Details ERK2 Application Details Handling References for anti-ERK2 Antibody (ABIN126833) Images
Specificity This antibody specifically recognizes the N-terminus of MAP kinase 2 (ERK2).
Characteristics Synonyms: Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2,MAPK2, MAPK1, Extracellular signal-regulated kinase 2
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Components incl. positive Control
Immunogen Peptide conjugated to KLHEpitope: N-terminus
Clone 6H3
Isotype IgG1

Target Details ERK2

Product Details anti-ERK2 Antibody Application Details Handling References for anti-ERK2 Antibody (ABIN126833) Images back to top
Antigen
Alternative Name ERK2 (MAPK1 Antibody Abstract)
Background Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.Synonyms: ERT1, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, PRKM1, PRKM2, p42-MAPK
Molecular Weight 42 kDa
Gene ID 5594
UniProt P28482
Research Area Stem Cells, Signaling, Inflammation
Pathways MAPK Signaling, RTK Signaling, Apoptosis, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-ERK2 Antibody Target Details ERK2 Handling References for anti-ERK2 Antibody (ABIN126833) Images back to top
Application Notes ELISA: Use at 0.05 μg/mL. Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated HepG2 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive Control Provided. Cell lysate from untreated HepG2Description: Cell lysate from untreated HepG2 cells, hepatocellular carcinoma (human)Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (minigel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.
Restrictions For Research Use only

Handling

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details References for anti-ERK2 Antibody (ABIN126833) Images back to top
Reconstitution Restore with 1.0 mL H2O (15 min, RT).
Buffer 1 mL2 x PBS containing PEG and Sucrose, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Expiry Date 12 months

References for anti-ERK2 Antibody (ABIN126833)

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling Images back to top
Product cited in:

Samuels, Karlo, Faruzzi, Pickering, Herrup, Sweatt, Saitta, Landreth: "Deletion of ERK2 mitogen-activated protein kinase identifies its key roles in cortical neurogenesis and cognitive function." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 27, pp. 6983-95, 2008

Images

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling References for anti-ERK2 Antibody (ABIN126833) back to top
Supplier Images
Western Blotting (WB) image for anti-ERK2 antibody (Mitogen-Activated Protein Kinase 1) (N-Term) (ABIN126833) anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (N-Term) antibody