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anti-Mouse (Murine) ERK2 antibody for Western Blotting

Recommended ERK2 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
  • ATMPK1
  • F14N23.9
  • F14N23_9
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • ATMPK2
  • MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2
  • T30E16.13
  • T30E16_13
  • mitogen-activated protein kinase homolog 2
  • ERK2
  • zERK2
  • MAPK1
  • ERK
  • ERT1
  • MAPK2
  • P42MAPK
  • PRKM1
  • PRKM2
  • p38
  • p40
  • p41
  • p41mapk
  • 9030612K14Rik
  • AA407128
  • AU018647
  • C78273
  • Erk2
  • Prkm1
  • p42mapk
  • erk
  • erk2
  • ert1
  • mapk
  • mpk1
  • xp42
  • mapk2
  • prkm1
  • prkm2
  • Xp42
  • mapk1b
  • mapk1
  • mapk1a
  • mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 2
  • mitogen activated protein kinase 1
  • ATMPK1
  • MPK1
  • MPK2
  • MAPK1
  • mapk1
  • Mapk1
  • PfMAP1
  • PVX_084965
  • Tsp_01601
  • mapk1-b
  • mapk1-a
Epitope
N-Term
58
22
15
11
9
8
8
8
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
332
141
112
50
30
19
14
11
10
9
8
7
5
5
2
2
2
2
2
1
1
1
Host
Mouse
164
157
17
3
2
Clonality
Monoclonal
Conjugate
This ERK2 antibody is un-conjugated
8
8
7
7
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
306
147
89
81
72
55
50
25
19
9
5
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN126833
$ 478.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0923712 ABIN1882240 WB Mouse IgG1, kappa AA 154-183 Log in to see 814CT5-4-2 5
3.0923712 ABIN1724654 ICC IHC ELISA WB Mouse IgG2a Log in to see 4C11 2
3.0923712 ABIN126832 EIA IP WB Mouse IgG1 C-Term Log in to see 6G11
3.0923712 ABIN967747 IF IP IHC WB Mouse IgG2b AA 219-358 Log in to see 33-ERK2 5
3.0923712 ABIN967748 IF IP IHC WB Mouse IgG2b AA 219-358 Log in to see 33-ERK2 5
3.0923712 ABIN967397 IP WB Mouse IgG1, kappa Log in to see G263-7 1
3.0923712 ABIN2787870 WB Rabbit C-Term Log in to see Polyclonal
3.0923712 ABIN2787871 WB Rabbit Middle Region Log in to see Polyclonal
3.0923712 ABIN1107133 IF IHC (p) WB Mouse IgG2a Log in to see 4C11 2
3.0923712 ABIN2788693 WB Rabbit C-Term Log in to see Polyclonal
3.0923712 ABIN3184547 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.0923712 ABIN2706125 WB Rabbit Center Log in to see Polyclonal
3.0923712 ABIN258758 ICC IHC (fro) IF IHC (p) IHC ELISA WB Mouse IgG2a Log in to see 4C11
3.0923712 ABIN1533683 ELISA WB Rabbit IgG Log in to see Polyclonal
3.0923712 ABIN438932 ICC IF IHC IHC (p) IP PLA WB Rabbit AA 310-360 Log in to see Polyclonal
3.0923712 ABIN3020728 IHC WB Rabbit IgG N-Term Log in to see Polyclonal
3.0923712 ABIN3031823 ELISA WB Mouse IgG1, kappa AA 154-183 Log in to see 814CT5-4-2
3.0923712 ABIN2467812 WB Chicken AA 232-360 Log in to see Polyclonal
3.0923712 ABIN4270497 IP WB Rabbit Log in to see T8
3.0923712 ABIN1873622 IF IHC WB Rabbit IgG N-Term Log in to see Polyclonal

Top referenced anti-ERK2 antibody for Western Blotting

Similar anti-ERK2 Antibodies

Application / Reactivity Human Mouse (Murine) Dog (Canine) Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 1 Antibodies
Simple Western (SimWes) 2 Antibodies
Western Blotting (WB) 296 Antibodies 30 Antibodies 129 Antibodies 106 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies 2 Antibodies 1 Antibodies 1 Antibodies
Luminex Assay (LMNX) 5 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 17 Antibodies 49 Antibodies

General

Antigen Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies
Epitope N-Term
(58), (22), (15), (11), (9), (8), (8), (8), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(332), (141), (112), (50), (30), (19), (14), (11), (10), (9), (8), (7), (5), (5), (2), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(164), (157), (17), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ERK2 antibody is un-conjugated
(8), (8), (7), (7), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(306), (147), (89), (81), (72), (55), (50), (25), (19), (9), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-ERK2 Antibody

Target Details ERK2 Application Details Handling References for anti-ERK2 Antibody (ABIN126833) Images
Specificity This antibody specifically recognizes the N-terminus of MAP kinase 2 (ERK2).
Characteristics Synonyms: Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2,MAPK2, MAPK1, Extracellular signal-regulated kinase 2
Purification Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Components incl. positive Control
Immunogen Peptide conjugated to KLHEpitope: N-terminus
Clone 6H3
Isotype IgG1

Target Details ERK2

Product Details anti-ERK2 Antibody Application Details Handling References for anti-ERK2 Antibody (ABIN126833) Images back to top
Antigen
Alternative Name ERK2 (MAPK1 Antibody Abstract)
Background Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.Synonyms: ERT1, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, PRKM1, PRKM2, p42-MAPK
Molecular Weight 42 kDa
Gene ID 5594
UniProt P28482
Research Area Stem Cells, Signaling, Inflammation
Pathways MAPK Signaling, RTK Signaling, Apoptosis, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-ERK2 Antibody Target Details ERK2 Handling References for anti-ERK2 Antibody (ABIN126833) Images back to top
Application Notes ELISA: Use at 0.05 μg/mL. Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated HepG2 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive Control Provided. Cell lysate from untreated HepG2Description: Cell lysate from untreated HepG2 cells, hepatocellular carcinoma (human)Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (minigel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.
Restrictions For Research Use only

Handling

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details References for anti-ERK2 Antibody (ABIN126833) Images back to top
Reconstitution Restore with 1.0 mL H2O (15 min, RT).
Buffer 1 mL2 x PBS containing PEG and Sucrose, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Expiry Date 12 months

References for anti-ERK2 Antibody (ABIN126833)

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling Images back to top
Product cited in:

Samuels, Karlo, Faruzzi, Pickering, Herrup, Sweatt, Saitta, Landreth: "Deletion of ERK2 mitogen-activated protein kinase identifies its key roles in cortical neurogenesis and cognitive function." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 27, pp. 6983-95, 2008

Images

Product Details anti-ERK2 Antibody Target Details ERK2 Application Details Handling References for anti-ERK2 Antibody (ABIN126833) back to top
Supplier Images
Western Blotting (WB) image for anti-ERK2 antibody (Mitogen-Activated Protein Kinase 1) (N-Term) (ABIN126833) anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (N-Term) antibody