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|Application / Reactivity||Human||Mouse (Murine)||Xenopus laevis||Cow (Bovine)||Chimpanzee||Chicken|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies||1 Antibodies|
|Simple Western (SimWes)||2 Antibodies|
|Western Blotting (WB)||306 Antibodies||136 Antibodies||5 Antibodies||16 Antibodies||1 Antibodies||9 Antibodies|
|Proximity Ligation Assay (PLA)||3 Antibodies||2 Antibodies||1 Antibodies||1 Antibodies|
|Luminex Assay (LMNX)||5 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies||3 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Immunoprecipitation (IP)||55 Antibodies|
|Antigen||Mitogen-Activated Protein Kinase 1 (MAPK1) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Chicken, Chimpanzee, Cow (Bovine), Human, Mouse (Murine), Xenopus laevis Alternatives|
|Conjugate||This ERK2 antibody is un-conjugated Alternatives|
Western Blotting (WB)
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Product Details anti-ERK2 AntibodyTarget Details ERK2 Application Details Handling Images
|Specificity||This antibody is expected to recognise an epitope corresponding to aa 9-19 of both reported protein isoforms of human ERK2 / MAPK1 protein.|
Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.
|Immunogen||Peptide with sequence CAAGPEMVRGQVF, from the internal region of the protein sequence|
Target Details ERK2Product Details anti-ERK2 Antibody Application Details Handling Images back to top
|Alternative Name||ERK2 (MAPK1 Antibody Abstract)|
|Background||ERK2 is activated by MEK2, which phosphorylates neighbouring threonine 183 and tyrosine 185 residues. ERK2 is a serine/threonine kinase that phosphorylates MAP2 and myelin basic protein. This kinase is an important proximal componant of the MAP kinase pathway involved in transmitting the signals from growth factors, neurotransmitters and hormones at the cell surface to the transcriptional events in the nucleus.Synonyms: ERT1, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, PRKM1, PRKM2, p42-MAPK|
|Gene ID||5594, 9606|
|Research Area||Inflammation, Signaling, Stem Cells|
|Pathways||MAPK Signaling, RTK Signaling, Apoptosis, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals|
Application DetailsProduct Details anti-ERK2 Antibody Target Details ERK2 Handling Images back to top
Peptide ELISA: 1/8000. Western Blot: 0.5 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ERK2 Antibody Target Details ERK2 Application Details Images back to top
|Buffer||Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|