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anti-Dog (Canine) GJA1 antibody for Western Blotting

Recommended GJA1 Antibody (supplied by: Log in to see )

Antigen
Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
  • cx43
  • gjal
  • oddd
  • dfnb38
  • connexin43
  • cx43a1
  • gja1
  • zfCx43.3
  • AU042049
  • AW546267
  • Cnx43
  • Cx43
  • Cx43alpha1
  • Gja-1
  • Npm1
  • AVSD3
  • CX43
  • DFNB38
  • GJAL
  • HLHS1
  • HSS
  • ODDD
  • connexin 43
  • gja1-A
  • Cnx46
  • Cx46
  • Gja-3
  • AI118175
  • Cnx32
  • Cx32
  • Gjb-1
  • gap junction protein, alpha 1, 43kDa
  • connexin 43
  • CONNEXIN 43 protein
  • connexin 43-like
  • gap junction protein, alpha 1
  • gap junction protein, alpha 3
  • gap junction protein, beta 1
  • gja1
  • cx43
  • CX43
  • CONNEXIN 43
  • LOC100359132
  • Gja1
  • GJA1
  • Gja3
  • Gjb1
Epitope
N-Term
66
20
20
16
15
12
10
8
7
7
6
6
6
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
257
195
175
55
25
22
21
19
17
17
10
10
7
7
7
5
4
4
4
3
3
3
3
2
2
1
1
1
Host
Rabbit
254
27
23
Clonality
Polyclonal
Conjugate
This GJA1 antibody is un-conjugated
5
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
238
121
104
73
43
19
14
13
12
7
7
5
2
2
Options
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Catalog No. ABIN2774853
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9599175 ABIN2774852 WB Rabbit Middle Region Log in to see Polyclonal 1
2.9599175 ABIN967717 IHC (f) IF IP WB Mouse IgG1 AA 252-270 Log in to see 2-Connexin 5
2.9599175 ABIN967718 IHC (f) IF IP WB Mouse IgG1 AA 252-270 Log in to see 2-Connexin 5
2.9599175 ABIN2704672 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
2.9599175 ABIN2704673 IP WB Rabbit C-Term Log in to see Polyclonal
2.9599175 ABIN2162266 WB Rabbit C-Term Log in to see Polyclonal
2.9599175 ABIN2461382 ELISA WB Rabbit Log in to see Polyclonal
2.9599175 ABIN2441121 WB Rabbit pSer368 Log in to see Polyclonal
2.9599175 ABIN634661 WB Rabbit N-Term Log in to see Polyclonal
2.9599175 ABIN634660 WB Rabbit Middle Region Log in to see Polyclonal
2.9599175 ABIN2561944 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
1 ABIN452424 WB Goat Internal Region Log in to see Polyclonal 1
1 ABIN372602 WB Rabbit IgG C-Term Log in to see Polyclonal 1
1 ABIN372603 WB Rabbit IgG pSer368 Log in to see Polyclonal 1
1 ABIN671451 IF (p) IHC (p) WB Rabbit IgG AA 232-282 Log in to see Polyclonal 2
1 ABIN318733 WB Rabbit IgG C-Term Log in to see Polyclonal 1
1 ABIN319138 WB Rabbit IgG pSer368 Log in to see Polyclonal 3
1 ABIN216407 WB Rabbit IgG AA 91-140 Log in to see Polyclonal
1 ABIN462695 ELISA WB Goat AA 333-346 Log in to see Polyclonal
1 ABIN468263 WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-GJA1 antibody for Western Blotting

Similar anti-GJA1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Dog (Canine) Rabbit Pig (Porcine)
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies 4 Antibodies 4 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 17 Antibodies 151 Antibodies 170 Antibodies 193 Antibodies 39 Antibodies 15 Antibodies 13 Antibodies
Immunoprecipitation (IP) 4 Antibodies

General

Antigen Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
Epitope N-Term
(66), (20), (20), (16), (15), (12), (10), (8), (7), (7), (6), (6), (6), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(257), (195), (175), (55), (25), (22), (21), (19), (17), (17), (10), (10), (7), (7), (7), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(254), (27), (23)
Clonality
Polyclonal
Conjugate This GJA1 antibody is un-conjugated
(5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(238), (121), (104), (73), (43), (19), (14), (13), (12), (7), (7), (5), (2), (2)
Pubmed 1 reference available
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Product Details anti-GJA1 Antibody

Target Details GJA1 Application Details Handling References for anti-GJA1 Antibody (ABIN2774853) Images
Blocking Peptide Blocking peptide for this product available: ABIN977415
Sequence LYLAHVFYVM RKEEKLNKKE EELKVAQTDG VNVDMHLKQI EIKKFKYGIE
Predicted Reactivity Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Chicken : 90 %
Characteristics This is a rabbit polyclonal antibody against GJA1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human GJA1

Target Details GJA1

Product Details anti-GJA1 Antibody Application Details Handling References for anti-GJA1 Antibody (ABIN2774853) Images back to top
Antigen
Alternative Name GJA1 (GJA1 Antibody Abstract)
Background Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.
Molecular Weight 42 kDa
Gene ID 2697
NCBI Accession NM_000165, NP_000156
UniProt Q53FJ6
Pathways MAPK Signaling, Myometrial Relaxation and Contraction

Application Details

Product Details anti-GJA1 Antibody Target Details GJA1 Handling References for anti-GJA1 Antibody (ABIN2774853) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 382 AA

Restrictions For Research Use only

Handling

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details References for anti-GJA1 Antibody (ABIN2774853) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-GJA1 Antibody (ABIN2774853)

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling Images back to top
Background publications

Gellhaus, Dong, Propson, Maass, Klein-Hitpass, Kibschull, Traub, Willecke, Perbal, Lye, Winterhager: "Connexin43 interacts with NOV: a possible mechanism for negative regulation of cell growth in choriocarcinoma cells." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36931-42, 2004

Images

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling References for anti-GJA1 Antibody (ABIN2774853) back to top
Supplier Images
Western Blotting (WB) image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (N-Term) (ABIN2774853) WB Suggested Anti-GJA1 Antibody
Positive Control: Lane1:641 µg rat stiatum
Western Blotting (WB) image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (N-Term) (ABIN2774853) WB Suggested Anti-GJA1
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500 <...
Western Blotting (WB) image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (N-Term) (ABIN2774853) Species+tissue/cell type: Total rat cardiac lysate1:8 µg total cardiac lysate2: 15 µg...