anti-Guinea Pig GJA1 antibody for Western Blotting

Recommended GJA1 Antibody (supplied by: Log in to see )

Antigen
Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
  • cx43
  • gjal
  • oddd
  • dfnb38
  • connexin43
  • cx43a1
  • gja1
  • zfCx43.3
  • AU042049
  • AW546267
  • Cnx43
  • Cx43
  • Cx43alpha1
  • Gja-1
  • Npm1
  • AVSD3
  • CX43
  • DFNB38
  • GJAL
  • HLHS1
  • HSS
  • ODDD
  • connexin 43
  • gja1-A
  • Cnx46
  • Cx46
  • Gja-3
  • AI118175
  • Cnx32
  • Cx32
  • Gjb-1
  • gap junction protein, alpha 1, 43kDa
  • connexin 43
  • CONNEXIN 43 protein
  • connexin 43-like
  • gap junction protein, alpha 1
  • gap junction protein, alpha 3
  • gap junction protein, beta 1
  • gja1
  • cx43
  • CX43
  • CONNEXIN 43
  • LOC100359132
  • Gja1
  • GJA1
  • Gja3
  • Gjb1
Epitope
N-Term
69
20
20
18
15
13
10
8
8
7
6
5
5
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish
281
191
173
52
22
22
20
16
16
15
10
9
7
6
6
5
4
4
4
3
3
3
3
2
1
1
1
1
Host
Rabbit
261
43
22
Clonality
Polyclonal
Conjugate
This GJA1 antibody is un-conjugated
5
5
5
4
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
Application
Western Blotting (WB)
242
119
102
82
45
29
20
14
13
8
7
5
2
2
2
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Catalog No. ABIN2774853
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2182703 ABIN2774852 WB Rabbit Middle Region Log in to see Polyclonal 1
3.2182703 ABIN1440033 IF WB Goat IgG C-Term Log in to see Polyclonal
3.2182703 ABIN1440034 IF WB Goat IgG C-Term Log in to see Polyclonal
3.2182703 ABIN1440035 IF WB Goat IgG C-Term Log in to see Polyclonal
1 ABIN462695 ELISA WB Goat AA 333-346 Log in to see Polyclonal
1 ABIN216407 WB Rabbit IgG AA 91-140 Log in to see Polyclonal
1 ABIN468263 WB Rabbit C-Term Log in to see Polyclonal
1 ABIN319138 WB Rabbit IgG pSer368 Log in to see Polyclonal 3
1 ABIN5551528 WB Rabbit IgG pSer368 Log in to see Polyclonal
1 ABIN2473077 WB Rabbit IgG pSer368 Log in to see Polyclonal 3
1 ABIN2621330 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 351-367 Log in to see Polyclonal
1 ABIN220025 IHC (fro) WB Mouse AA 131-142 Log in to see

General

Antigen Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
Epitope N-Term
(69), (20), (20), (18), (15), (13), (10), (8), (8), (7), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish
(281), (191), (173), (52), (22), (22), (20), (16), (16), (15), (10), (9), (7), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(261), (43), (22)
Clonality
Polyclonal
Conjugate This GJA1 antibody is un-conjugated
(5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(242), (119), (102), (82), (45), (29), (20), (14), (13), (8), (7), (5), (2), (2), (2)
Pubmed 1 reference available
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Product Details anti-GJA1 Antibody

Target Details GJA1 Application Details Handling References for anti-GJA1 antibody (ABIN2774853) Images
Blocking Peptide Blocking peptide for this product available: ABIN977415
Sequence LYLAHVFYVM RKEEKLNKKE EELKVAQTDG VNVDMHLKQI EIKKFKYGIE
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 92%
Characteristics This is a rabbit polyclonal antibody against GJA1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human GJA1

Target Details GJA1

Product Details anti-GJA1 Antibody Application Details Handling References for anti-GJA1 antibody (ABIN2774853) Images back to top
Antigen
Alternative Name GJA1 (GJA1 Antibody Abstract)
Background Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.
Alias Symbols: HSS, CX43, GJAL, ODDD, AVSD3, HLHS1, DFNB38
Protein Interaction Partner: UBC, STAMBP, EPS15, UBQLN4, PSMD4, PSMD2, S100A1, GJA1, CNST, NEDD4, TJP1, HGS, TSG101, SGSM3, GJA5, MAPK7, MAPK3, CAV1, CSNK1D, SRC, PRKCG, PRKCE, PRKCB, PRKCA, MAPK1, NOV, CSK, GJA3,
Protein Size: 382
Molecular Weight 42 kDa
Gene ID 2697
NCBI Accession NM_000165, NP_000156
UniProt Q53FJ6
Pathways MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization

Application Details

Product Details anti-GJA1 Antibody Target Details GJA1 Handling References for anti-GJA1 antibody (ABIN2774853) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 382 AA

Restrictions For Research Use only

Handling

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details References for anti-GJA1 antibody (ABIN2774853) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GJA1 antibody (ABIN2774853)

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling Images back to top
Background publications

Gellhaus, Dong, Propson, Maass, Klein-Hitpass, Kibschull, Traub, Willecke, Perbal, Lye, Winterhager: "Connexin43 interacts with NOV: a possible mechanism for negative regulation of cell growth in choriocarcinoma cells." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36931-42, 2004

Images

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling References for anti-GJA1 antibody (ABIN2774853) back to top
Supplier Images
Western Blotting (WB) image for anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) (N-Term) antibody (ABIN2774853) WB Suggested Anti-GJA1
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500 <...