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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Chicken||Cow (Bovine)||Dog (Canine)||Zebrafish (Danio rerio)||Guinea Pig|
|Immunofluorescence (fixed cells) (IF/ICC)||6 Antibodies||4 Antibodies||4 Antibodies||1 Antibodies||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||16 Antibodies||143 Antibodies||161 Antibodies||184 Antibodies||37 Antibodies||20 Antibodies||5 Antibodies||11 Antibodies|
|Antigen||Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies|
|Reactivity||Cow (Bovine), Dog (Canine), Chicken, Guinea Pig, Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio) Alternatives|
|Conjugate||This GJA1 antibody is un-conjugated Alternatives|
Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-GJA1 AntibodyTarget Details GJA1 Application Details Handling References for anti-GJA1 Antibody (ABIN372603) Images
|Specificity||Specific for the ~43k Connexin-43 protein phosphorylated at Ser368. Immunolabeling is blocked by λ-phosphatase treatment.|
Species reactivity (expected):Bovine, Canine, Chicken, Guinea Pig, Human, Mouse and Zebrafish.
Species reactivity (tested):Rat.
|Purification||Sequential Chromatography on phospho- and dephosphopeptide affinity columns.|
|Immunogen||Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser368 of rat Connexin-43.|
Target Details GJA1Product Details anti-GJA1 Antibody Application Details Handling References for anti-GJA1 Antibody (ABIN372603) Images back to top
|Alternative Name||GJA1 / Cx43 (GJA1 Antibody Abstract)|
|Background||Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser368 by protein kinase C (Yogo et al., 2002, Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).Synonyms: Connexin-43, GJAL, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein|
|Pathways||MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization|
Application DetailsProduct Details anti-GJA1 Antibody Target Details GJA1 Handling References for anti-GJA1 Antibody (ABIN372603) Images back to top
Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-GJA1 Antibody Target Details GJA1 Application Details References for anti-GJA1 Antibody (ABIN372603) Images back to top
|Buffer||10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody undiluted (in aliquots) at-20 °C.|
References for anti-GJA1 Antibody (ABIN372603)Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling Images back to top
Aronica, Gorter, Jansen, Leenstra, Yankaya, Troost: "Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex." in: Acta neuropathologica, Vol. 101, Issue 5, pp. 449-59, 2001
ImagesProduct Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling References for anti-GJA1 Antibody (ABIN372603) back to top