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anti-Guinea Pig GJA1 antibody for Western Blotting

Recommended GJA1 Antibody (supplied by: Log in to see )

Antigen
Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
  • cx43
  • gjal
  • oddd
  • dfnb38
  • connexin43
  • cx43a1
  • gja1
  • zfCx43.3
  • AU042049
  • AW546267
  • Cnx43
  • Cx43
  • Cx43alpha1
  • Gja-1
  • Npm1
  • AVSD3
  • CX43
  • DFNB38
  • GJAL
  • HLHS1
  • HSS
  • ODDD
  • connexin 43
  • gja1-A
  • Cnx46
  • Cx46
  • Gja-3
  • AI118175
  • Cnx32
  • Cx32
  • Gjb-1
  • gap junction protein, alpha 1, 43kDa
  • connexin 43
  • CONNEXIN 43 protein
  • connexin 43-like
  • gap junction protein, alpha 1
  • gap junction protein, alpha 3
  • gap junction protein, beta 1
  • gja1
  • cx43
  • CX43
  • CONNEXIN 43
  • LOC100359132
  • Gja1
  • GJA1
  • Gja3
  • Gjb1
Epitope
pSer368
66
20
17
16
15
12
10
8
7
6
6
5
5
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Guinea Pig, Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
255
185
166
52
22
21
19
18
17
13
10
9
7
6
5
4
4
4
4
3
3
3
3
2
1
1
1
1
Host
Rabbit
244
34
22
Clonality
Polyclonal
Conjugate
This GJA1 antibody is un-conjugated
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Application
Western Blotting (WB)
228
116
98
76
42
20
19
14
12
8
7
5
2
2
Supplier
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Catalog No. ABIN372603
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN319138 WB Rabbit IgG pSer368 Log in to see Polyclonal 3
1 ABIN216407 WB Rabbit IgG AA 91-140 Log in to see Polyclonal
1 ABIN462695 ELISA WB Goat AA 333-346 Log in to see Polyclonal
1 ABIN468263 WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2621330 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 351-367 Log in to see Polyclonal
1 ABIN220025 IHC (fro) WB Mouse AA 131-142 Log in to see
1 ABIN2473077 WB Rabbit IgG pSer368 Log in to see Polyclonal 3
1 ABIN1440033 IF WB Goat IgG C-Term Log in to see Polyclonal
1 ABIN1440035 IF WB Goat IgG C-Term Log in to see Polyclonal
1 ABIN1440034 IF WB Goat IgG C-Term Log in to see Polyclonal

Top referenced anti-GJA1 antibody for Western Blotting

Similar anti-GJA1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Cow (Bovine) Dog (Canine) Zebrafish (Danio rerio) Guinea Pig
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies 4 Antibodies 4 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 16 Antibodies 143 Antibodies 161 Antibodies 184 Antibodies 37 Antibodies 20 Antibodies 5 Antibodies 11 Antibodies

General

Antigen Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
Epitope pSer368
(66), (20), (17), (16), (15), (12), (10), (8), (7), (6), (6), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Chicken, Guinea Pig, Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
(255), (185), (166), (52), (22), (21), (19), (18), (17), (13), (10), (9), (7), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(244), (34), (22)
Clonality
Polyclonal
Conjugate This GJA1 antibody is un-conjugated
(5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(228), (116), (98), (76), (42), (20), (19), (14), (12), (8), (7), (5), (2), (2)
Pubmed 1 reference available
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Product Details anti-GJA1 Antibody

Target Details GJA1 Application Details Handling References for anti-GJA1 Antibody (ABIN372603) Images
Specificity Specific for the ~43k Connexin-43 protein phosphorylated at Ser368. Immunolabeling is blocked by λ-phosphatase treatment.
Cross-Reactivity (Details) Species reactivity (expected):Bovine, Canine, Chicken, Guinea Pig, Human, Mouse and Zebrafish.
Species reactivity (tested):Rat.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser368 of rat Connexin-43.
Isotype IgG

Target Details GJA1

Product Details anti-GJA1 Antibody Application Details Handling References for anti-GJA1 Antibody (ABIN372603) Images back to top
Antigen
Alternative Name GJA1 / Cx43 (GJA1 Antibody Abstract)
Background Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser368 by protein kinase C (Yogo et al., 2002, Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).Synonyms: Connexin-43, GJAL, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein
Gene ID 2697
NCBI Accession NP_000156
UniProt P17302
Pathways MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization

Application Details

Product Details anti-GJA1 Antibody Target Details GJA1 Handling References for anti-GJA1 Antibody (ABIN372603) Images back to top
Application Notes Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details References for anti-GJA1 Antibody (ABIN372603) Images back to top
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-GJA1 Antibody (ABIN372603)

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling Images back to top
Background publications

Aronica, Gorter, Jansen, Leenstra, Yankaya, Troost: "Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex." in: Acta neuropathologica, Vol. 101, Issue 5, pp. 449-59, 2001

Images

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling References for anti-GJA1 Antibody (ABIN372603) back to top
Supplier Images
 image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (pSer368) (ABIN372603) anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) (pSer368) antibody