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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Flow Cytometry (FACS)||3 Antibodies|
|Immunochromatography (IC)||4 Antibodies|
|Immunocytochemistry (ICC)||15 Antibodies|
|Immunofluorescence (IF)||36 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies|
|Immunohistochemistry (IHC)||105 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||49 Antibodies|
|Immunoprecipitation (IP)||8 Antibodies|
|Light Microscopy (LM)||2 Antibodies|
|Western Blotting (WB)||172 Antibodies|
|Antigen||Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies|
|Conjugate||This GJA1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-GJA1 AntibodyTarget Details GJA1 Application Details Handling References for anti-GJA1 Antibody (ABIN357085) Images
|Specificity||This antibody detects Connexin 43 (GJA1) at N-term.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human GJA1.|
Target Details GJA1Product Details anti-GJA1 Antibody Application Details Handling References for anti-GJA1 Antibody (ABIN357085) Images back to top
|Alternative Name||GJA1 / Cx43 (GJA1 Antibody Abstract)|
|Background||Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.Synonyms: Connexin-43, GJAL, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein|
|Molecular Weight||42877 Da|
|Gene ID||2697, 9606|
Application DetailsProduct Details anti-GJA1 Antibody Target Details GJA1 Handling References for anti-GJA1 Antibody (ABIN357085) Images back to top
ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-GJA1 Antibody Target Details GJA1 Application Details References for anti-GJA1 Antibody (ABIN357085) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.|
References for anti-GJA1 Antibody (ABIN357085)Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling Images back to top
Contreras, Sáez, Bukauskas et al.: "Gating and regulation of connexin 43 (Cx43) hemichannels." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 20, pp. 11388-93, 2003 (PubMed).
Spinella, Rosanò, Di Castro et al.: "Endothelin-1 decreases gap junctional intercellular communication by inducing phosphorylation of connexin 43 in human ovarian carcinoma cells." in: The Journal of biological chemistry, Vol. 278, Issue 42, pp. 41294-301, 2003 (PubMed).
Qin, Shao, Igdoura et al.: "Lysosomal and proteasomal degradation play distinct roles in the life cycle of Cx43 in gap junctional intercellular communication-deficient and -competent breast tumor cells." in: The Journal of biological chemistry, Vol. 278, Issue 32, pp. 30005-14, 2003 (PubMed).
Ma, Ma, Sui et al.: "Signal transduction of gap junctional genes, connexin32, connexin43 in human hepatocarcinogenesis." in: World journal of gastroenterology : WJG, Vol. 9, Issue 5, pp. 946-50, 2003 (PubMed).
Cameron, Malik, Akaike et al.: "Regulation of epidermal growth factor-induced connexin 43 gap junction communication by big mitogen-activated protein kinase1/ERK5 but not ERK1/2 kinase activation." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 18682-8, 2003 (PubMed).
ImagesProduct Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling References for anti-GJA1 Antibody (ABIN357085) back to top