You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human GJA1 antibody for Enzyme Immunoassay

Recommended GJA1 Antibody (supplied by: Log in to see )

Antigen
Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
  • AI118175
  • AU042049
  • AVSD3
  • AW546267
  • Cnx32
  • Cnx43
  • Cnx46
  • connexin43
  • connexin 43
  • Cx32
  • cx43
  • CX43
  • Cx43
  • cx43a1
  • Cx43alpha1
  • Cx46
  • DFNB38
  • dfnb38
  • Gja-1
  • Gja-3
  • gja1
  • gja1-A
  • GJAL
  • gjal
  • Gjb-1
  • HLHS1
  • HSS
  • Npm1
  • ODDD
  • oddd
  • zfCx43.3
Epitope
N-Term
66
22
19
16
13
10
10
9
7
7
7
6
6
5
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
245
167
143
44
20
15
14
12
11
10
9
7
6
6
5
4
4
3
3
3
3
3
2
1
1
1
Host
Rabbit
249
28
22
Clonality
Polyclonal
Conjugate
This GJA1 antibody is un-conjugated
6
5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
223
117
103
61
46
22
12
10
10
9
7
2
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN357085
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN265368 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-GJA1 antibody for Enzyme Immunoassay

Similar anti-GJA1 Antibodies

Application / Reactivity Human
ELISA 76 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 19 Antibodies
Immunofluorescence (IF) 40 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 111 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 56 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Light Microscopy (LM) 2 Antibodies
Western Blotting (WB) 176 Antibodies

General

Antigen Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
Epitope N-Term
(66), (22), (19), (16), (13), (10), (10), (9), (7), (7), (7), (6), (6), (5), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(245), (167), (143), (44), (20), (15), (14), (12), (11), (10), (9), (7), (6), (6), (5), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(249), (28), (22)
Clonality
Polyclonal
Conjugate This GJA1 antibody is un-conjugated
(6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(223), (117), (103), (61), (46), (22), (12), (10), (10), (9), (7), (2), (2), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-GJA1 Antibody

Target Details GJA1 Application Details Handling References for anti-GJA1 Antibody (ABIN357085) Images
Specificity This antibody detects Connexin 43 (GJA1) at N-term.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human GJA1.
Isotype Ig

Target Details GJA1

Product Details anti-GJA1 Antibody Application Details Handling References for anti-GJA1 Antibody (ABIN357085) Images back to top
Antigen
Alternative Name GJA1 / Cx43 (GJA1 Antibody Abstract)
Background Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.Synonyms: Connexin-43, GJAL, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein
Molecular Weight 42877 Da
Gene ID 2697, 9606
UniProt P17302
Pathways MAPK Signaling

Application Details

Product Details anti-GJA1 Antibody Target Details GJA1 Handling References for anti-GJA1 Antibody (ABIN357085) Images back to top
Application Notes ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details References for anti-GJA1 Antibody (ABIN357085) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-GJA1 Antibody (ABIN357085)

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling Images back to top
Background publications

Spinella, Rosanò, Di Castro, Nicotra, Natali, Bagnato: "Endothelin-1 decreases gap junctional intercellular communication by inducing phosphorylation of connexin 43 in human ovarian carcinoma cells." in: The Journal of biological chemistry, Vol. 278, Issue 42, pp. 41294-301, 2003

Contreras, Sáez, Bukauskas, Bennett: "Gating and regulation of connexin 43 (Cx43) hemichannels." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 20, pp. 11388-93, 2003

Qin, Shao, Igdoura, Alaoui-Jamali, Laird: "Lysosomal and proteasomal degradation play distinct roles in the life cycle of Cx43 in gap junctional intercellular communication-deficient and -competent breast tumor cells." in: The Journal of biological chemistry, Vol. 278, Issue 32, pp. 30005-14, 2003

Cameron, Malik, Akaike, Lerner-Marmarosh, Yan, Lee, Abe, Yang: "Regulation of epidermal growth factor-induced connexin 43 gap junction communication by big mitogen-activated protein kinase1/ERK5 but not ERK1/2 kinase activation." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 18682-8, 2003

Ma, Ma, Sui, Wang, Wang: "Signal transduction of gap junctional genes, connexin32, connexin43 in human hepatocarcinogenesis." in: World journal of gastroenterology : WJG, Vol. 9, Issue 5, pp. 946-50, 2003

Images

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling References for anti-GJA1 Antibody (ABIN357085) back to top
Supplier Images
 image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (N-Term) (ABIN357085) anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) (N-Term) antibody
 image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (N-Term) (ABIN357085) anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) (N-Term) antibody (Image 2)