anti-Human GJA1 antibody for Immunoprecipitation

Recommended GJA1 Antibody (supplied by: Log in to see )

Antigen
Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
  • cx43
  • gjal
  • oddd
  • dfnb38
  • connexin43
  • cx43a1
  • gja1
  • zfCx43.3
  • AU042049
  • AW546267
  • Cnx43
  • Cx43
  • Cx43alpha1
  • Gja-1
  • Npm1
  • AVSD3
  • CX43
  • DFNB38
  • GJAL
  • HLHS1
  • HSS
  • ODDD
  • connexin 43
  • gja1-A
  • Cnx46
  • Cx46
  • Gja-3
  • AI118175
  • Cnx32
  • Cx32
  • Gjb-1
  • gap junction protein, alpha 1, 43kDa
  • connexin 43
  • CONNEXIN 43 protein
  • connexin 43-like
  • gap junction protein, alpha 1
  • gap junction protein, alpha 3
  • gap junction protein, beta 1
  • gja1
  • cx43
  • CX43
  • CONNEXIN 43
  • LOC100359132
  • Gja1
  • GJA1
  • Gja3
  • Gjb1
Epitope
AA 252-270
67
21
18
16
15
13
10
8
8
7
6
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
260
190
171
52
22
21
21
17
16
16
10
10
7
7
6
5
5
4
4
3
3
3
3
2
1
1
1
1
Host
Mouse
250
33
22
Clonality
Monoclonal
Conjugate
This GJA1 antibody is un-conjugated
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
233
116
99
77
41
20
19
14
11
8
7
5
2
1
Options
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Catalog No. ABIN967717
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967718 IHC (f) IF IP WB Mouse IgG1 AA 252-270 Log in to see 2-Connexin 5
1 ABIN2704673 IP WB Rabbit C-Term Log in to see Polyclonal
1 ABIN1858579 ICC IHC IP WB Rabbit IgG AA 180-382 Log in to see Polyclonal
1 ABIN1495894 ICC IHC (p) IP WB Sepharose Rabbit C-Term Log in to see Polyclonal
1 ABIN1495893 ICC IHC (p) IP WB Magnetic Particles Rabbit C-Term Log in to see Polyclonal
1 ABIN2621332 IP WB Rabbit C-Term Log in to see Polyclonal
1 ABIN1690046 IP Magnetic Particles Rabbit Log in to see Polyclonal
1 ABIN1690394 IP Sepharose Rabbit Log in to see Polyclonal

Top referenced anti-GJA1 antibody for Immunoprecipitation

Similar anti-GJA1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Dog (Canine)
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies 4 Antibodies 4 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 148 Antibodies 166 Antibodies 189 Antibodies 37 Antibodies 20 Antibodies
Immunoprecipitation (IP) 4 Antibodies 4 Antibodies 9 Antibodies 9 Antibodies

General

Antigen Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
Epitope AA 252-270
(67), (21), (18), (16), (15), (13), (10), (8), (8), (7), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(260), (190), (171), (52), (22), (21), (21), (17), (16), (16), (10), (10), (7), (7), (6), (5), (5), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Host Mouse
(250), (33), (22)
Clonality (Clone)
Monoclonal   ( )
Conjugate This GJA1 antibody is un-conjugated
(5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(233), (116), (99), (77), (41), (20), (19), (14), (11), (8), (7), (5), (2), (1)
Pubmed 5 references available
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Product Details anti-GJA1 Antibody

Target Details GJA1 Application Details Handling ProductDetails: References for anti-GJA1 antibody (ABIN967717) Images
Cross-Reactivity Chicken, Dog (Canine), Human, Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat Connexin-43 aa. 252-270
Clone 2-Connexin
Isotype IgG1

Target Details GJA1

Product Details anti-GJA1 Antibody Application Details Handling ProductDetails: References for anti-GJA1 antibody (ABIN967717) Images back to top
Antigen
Alternative Name Connexin-43 (GJA1 Antibody Abstract)
Background Gap junctions are intercellular protein pores. They enable cell-to-cell communication by allowing passage of ions and other small molecules. The subunits of gap junction channels are assembled from a family of proteins called connexins. Individual connexin molecules join to make hexameric hemichannels termed connexons, these structures dock to connexons on neighboring cells, forming gap junction pores. Connexin-43 is a member of the connexin family possessing four transmembrane regions, with cytoplasmic amino and carboxyl terminals. It undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. In addition, it is believed that phosphorylation of connexin-43 plays a regulatory role both in the assembly of connexons and in gating activity at the gap junction. This antibody is routinely tested by western blot analysis.
Molecular Weight 43 kDa
Research Area Extracellular Matrix, Cytoskeleton, Signaling
Pathways MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization

Application Details

Product Details anti-GJA1 Antibody Target Details GJA1 Handling ProductDetails: References for anti-GJA1 antibody (ABIN967717) Images back to top
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details ProductDetails: References for anti-GJA1 antibody (ABIN967717) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

ProductDetails: References for anti-GJA1 antibody (ABIN967717)

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling Images back to top
Product cited in:

Giepmans, Hengeveld, Postma, Moolenaar: "Interaction of c-Src with gap junction protein connexin-43. Role in the regulation of cell-cell communication." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 8544-9, 2001

Toyofuku, Akamatsu, Zhang, Kuzuya, Tada, Hori: "c-Src regulates the interaction between connexin-43 and ZO-1 in cardiac myocytes." in: The Journal of biological chemistry, Vol. 276, Issue 3, pp. 1780-8, 2001

Lucke, Choudhry, Thom, Selmer, Burden, Hodgins: "Upregulation of connexin 26 is a feature of keratinocyte differentiation in hyperproliferative epidermis, vaginal epithelium, and buccal epithelium." in: The Journal of investigative dermatology, Vol. 112, Issue 3, pp. 354-61, 1999

Laird, Castillo, Kasprzak: "Gap junction turnover, intracellular trafficking, and phosphorylation of connexin43 in brefeldin A-treated rat mammary tumor cells." in: The Journal of cell biology, Vol. 131, Issue 5, pp. 1193-203, 1996

Loo, Berestecky, Kanemitsu, Lau: "pp60src-mediated phosphorylation of connexin 43, a gap junction protein." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12751-61, 1995

Images

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling ProductDetails: References for anti-GJA1 antibody (ABIN967717) back to top
Supplier Images
Western Blotting (WB) image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (AA 252-270) (ABIN967717) Western blot analysis of Connexin-43 on a rat cerebrum lysate. Lane 1: 1:250, lane 2:...
Immunofluorescence (IF) image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (AA 252-270) (ABIN967717) Immunofluorescence staining on WI38 cells (human lung).