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anti-Human GJA1 antibody for Western Blotting

Recommended GJA1 Antibody (supplied by: Log in to see )

Antigen
Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
  • AI118175
  • AU042049
  • AVSD3
  • AW546267
  • Cnx32
  • Cnx43
  • Cnx46
  • connexin43
  • connexin 43
  • Cx32
  • cx43
  • CX43
  • Cx43
  • cx43a1
  • Cx43alpha1
  • Cx46
  • DFNB38
  • dfnb38
  • Gja-1
  • Gja-3
  • gja1
  • gja1-A
  • GJAL
  • gjal
  • Gjb-1
  • HLHS1
  • HSS
  • Npm1
  • ODDD
  • oddd
  • zfCx43.3
Epitope
N-Term
61
22
18
16
13
10
10
9
7
7
7
6
6
5
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
226
156
134
42
20
13
13
10
9
9
9
7
6
6
4
4
4
3
3
3
3
2
2
1
1
1
Host
Rabbit
236
21
21
Clonality
Polyclonal
Conjugate
This GJA1 antibody is un-conjugated
6
5
4
4
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
214
108
96
52
40
16
12
10
9
7
3
2
2
2
Supplier
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Catalog No. ABIN2774853
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13029787 ABIN2774852 WB Rabbit Middle Region Log in to see Polyclonal 1
0.13029787 ABIN388369 IHC (p) WB Rabbit Ig AA 107-138, N-Term Log in to see Polyclonal 9
0.13029787 ABIN1531807 IHC ELISA WB Rabbit IgG pSer367 Log in to see Polyclonal 1
0.13029787 ABIN1532837 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.13029787 ABIN967717 IHC (f) IF IP WB Mouse IgG1 AA 252-270 Log in to see 2-Connexin 5
0.13029787 ABIN967718 IHC (f) IF IP WB Mouse IgG1 AA 252-270 Log in to see 2-Connexin 5
0.13029787 ABIN1533240 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13029787 ABIN1687235 IHC (p) WB Rabbit IgG 3rd Cytoplasmic Domain Log in to see Polyclonal
0.13029787 ABIN2704671 IHC WB Rabbit C-Term, pSer368 Log in to see Polyclonal
0.13029787 ABIN2704673 IP WB Rabbit C-Term Log in to see Polyclonal
0.13029787 ABIN2704672 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
0.13029787 ABIN3044320 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 351-367, C-Term Log in to see Polyclonal
0.13029787 ABIN498754 IHC (p) WB Rabbit pSer367 Log in to see Polyclonal
0.13029787 ABIN1107367 IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.13029787 ABIN498634 IHC (p) WB Rabbit Log in to see Polyclonal
0.13029787 ABIN1870206 IHC WB Rabbit IgG pSer368 Log in to see Polyclonal
0.13029787 ABIN2461382 ELISA WB Rabbit Log in to see Polyclonal
0.13029787 ABIN1872816 IHC WB Rabbit IgG Log in to see Polyclonal
0.13029787 ABIN1872817 IHC WB Rabbit IgG Log in to see Polyclonal
0.13029787 ABIN2160249 WB Rabbit Ig Fraction N-Term Log in to see Polyclonal

Top referenced anti-GJA1 antibody for Western Blotting

Similar anti-GJA1 Antibodies

Application / Reactivity Cow (Bovine) Mouse (Murine) Rat (Rattus) Human Dog (Canine) Pig (Porcine) Rabbit
Western Blotting (WB) 11 Antibodies 114 Antibodies 137 Antibodies 171 Antibodies 30 Antibodies 9 Antibodies 7 Antibodies
Light Microscopy (LM) 2 Antibodies 2 Antibodies 2 Antibodies
Immunoprecipitation (IP) 3 Antibodies 8 Antibodies 5 Antibodies 6 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies

General

Antigen Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
Epitope N-Term
(61), (22), (18), (16), (13), (10), (10), (9), (7), (7), (7), (6), (6), (5), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(226), (156), (134), (42), (20), (13), (13), (10), (9), (9), (9), (7), (6), (6), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(236), (21), (21)
Clonality
Polyclonal
Conjugate This GJA1 antibody is un-conjugated
(6), (5), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(214), (108), (96), (52), (40), (16), (12), (10), (9), (7), (3), (2), (2), (2)
Pubmed 1 reference available
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Product Details anti-GJA1 Antibody

Target Details GJA1 Application Details Handling References for anti-GJA1 Antibody (ABIN2774853) Images
Blocking Peptide Blocking peptide for this product available: ABIN977415
Sequence LYLAHVFYVM RKEEKLNKKE EELKVAQTDG VNVDMHLKQI EIKKFKYGIE
Predicted Reactivity Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Chicken : 90 %
Characteristics This is a rabbit polyclonal antibody against GJA1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human GJA1

Target Details GJA1

Product Details anti-GJA1 Antibody Application Details Handling References for anti-GJA1 Antibody (ABIN2774853) Images back to top
Antigen
Alternative Name GJA1 (GJA1 Antibody Abstract)
Background Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.
Molecular Weight 42 kDa
Gene ID 2697
NCBI Accession NM_000165, NP_000156
UniProt Q53FJ6
Pathways MAPK Signaling

Application Details

Product Details anti-GJA1 Antibody Target Details GJA1 Handling References for anti-GJA1 Antibody (ABIN2774853) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 382 AA

Restrictions For Research Use only

Handling

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details References for anti-GJA1 Antibody (ABIN2774853) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-GJA1 Antibody (ABIN2774853)

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling Images back to top
Background publications

Gellhaus, Dong, Propson, Maass, Klein-Hitpass, Kibschull, Traub, Willecke, Perbal, Lye, Winterhager: "Connexin43 interacts with NOV: a possible mechanism for negative regulation of cell growth in choriocarcinoma cells." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36931-42, 2004

Images

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling References for anti-GJA1 Antibody (ABIN2774853) back to top
Supplier Images
Western Blotting (WB) image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (N-Term) (ABIN2774853) WB Suggested Anti-GJA1 Antibody
Positive Control: Lane1:641 µg rat stiatum
Western Blotting (WB) image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (N-Term) (ABIN2774853) WB Suggested Anti-GJA1
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500 <...
Western Blotting (WB) image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (N-Term) (ABIN2774853) Species+tissue/cell type: Total rat cardiac lysate1:8 µg total cardiac lysate2: 15 µg...