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anti-Human GJA1 antibody for Western Blotting

Recommended GJA1 Antibody (supplied by: Log in to see )

Antigen
Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
  • cx43
  • gjal
  • oddd
  • dfnb38
  • connexin43
  • cx43a1
  • gja1
  • zfCx43.3
  • AU042049
  • AW546267
  • Cnx43
  • Cx43
  • Cx43alpha1
  • Gja-1
  • Npm1
  • AVSD3
  • CX43
  • DFNB38
  • GJAL
  • HLHS1
  • HSS
  • ODDD
  • connexin 43
  • gja1-A
  • Cnx46
  • Cx46
  • Gja-3
  • AI118175
  • Cnx32
  • Cx32
  • Gjb-1
  • gap junction protein, alpha 1, 43kDa
  • connexin 43
  • CONNEXIN 43 protein
  • connexin 43-like
  • gap junction protein, alpha 1
  • gap junction protein, alpha 3
  • gap junction protein, beta 1
  • gja1
  • cx43
  • CX43
  • CONNEXIN 43
  • LOC100359132
  • Gja1
  • GJA1
  • Gja3
  • Gjb1
Epitope
AA 107-138, N-Term
66
19
18
16
15
11
10
8
7
6
6
5
5
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
255
186
167
53
22
22
20
18
17
14
10
9
7
6
5
5
4
4
4
3
3
3
3
2
1
1
1
1
Host
Rabbit
244
34
22
Clonality
Polyclonal
Conjugate
This GJA1 antibody is un-conjugated
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
228
116
98
75
42
20
19
14
12
8
7
5
2
2
Options
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Catalog No. ABIN388369
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0741818 ABIN2774852 WB Rabbit Middle Region Log in to see Polyclonal 1
3.0741818 ABIN2774853 WB Rabbit N-Term Log in to see Polyclonal 1
3.0741818 ABIN1531807 IHC ELISA WB Rabbit IgG pSer367 Log in to see Polyclonal 1
3.0741818 ABIN1532837 ELISA WB Rabbit IgG Log in to see Polyclonal 1
3.0741818 ABIN967717 IHC (f) IF IP WB Mouse IgG1 AA 252-270 Log in to see 2-Connexin 5
3.0741818 ABIN967718 IHC (f) IF IP WB Mouse IgG1 AA 252-270 Log in to see 2-Connexin 5
3.0741818 ABIN3184034 ELISA IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal
3.0741818 ABIN3181970 ELISA IHC WB Rabbit IgG pSer368 Log in to see Polyclonal
3.0741818 ABIN1533240 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0741818 ABIN1687235 IHC (p) WB Rabbit IgG 3rd Cytoplasmic Domain Log in to see Polyclonal
3.0741818 ABIN3043758 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 351-367, C-Term Log in to see Polyclonal
3.0741818 ABIN2704671 IHC WB Rabbit C-Term, pSer368 Log in to see Polyclonal
3.0741818 ABIN2704672 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
3.0741818 ABIN2704673 IP WB Rabbit C-Term Log in to see Polyclonal
3.0741818 ABIN498754 IHC (p) WB Rabbit pSer367 Log in to see Polyclonal
3.0741818 ABIN1107367 IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
3.0741818 ABIN1870206 IHC WB Rabbit IgG pSer368 Log in to see Polyclonal
3.0741818 ABIN498634 IHC (p) WB Rabbit Log in to see Polyclonal
3.0741818 ABIN1872816 FACS IHC WB Rabbit IgG Log in to see Polyclonal
3.0741818 ABIN1872817 IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-GJA1 antibody for Western Blotting

Similar anti-GJA1 Antibodies

Application / Reactivity Human
ELISA 73 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 20 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 17 Antibodies
Immunofluorescence (IF) 38 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 14 Antibodies
Immunohistochemistry (IHC) 106 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 75 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 184 Antibodies

General

Antigen Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
Epitope AA 107-138, N-Term
(66), (19), (18), (16), (15), (11), (10), (8), (7), (6), (6), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(255), (186), (167), (53), (22), (22), (20), (18), (17), (14), (10), (9), (7), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(244), (34), (22)
Clonality (Clone)
Polyclonal   ( )
Conjugate This GJA1 antibody is un-conjugated
(5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(228), (116), (98), (75), (42), (20), (19), (14), (12), (8), (7), (5), (2), (2)
Pubmed 9 references available
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Product Details anti-GJA1 Antibody

Target Details GJA1 Application Details Handling References for anti-GJA1 Antibody (ABIN388369) Images
Specificity This Connexin 43 Antibody antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114~144 amino acids from the N-terminal region of human GJA1.
Predicted Reactivity Cow (Bovine),Xenopus laevis
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This Connexin 43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-138 AA from the N-terminal region of human Connexin 43.
Clone RB01369
Isotype Ig

Target Details GJA1

Product Details anti-GJA1 Antibody Application Details Handling References for anti-GJA1 Antibody (ABIN388369) Images back to top
Antigen
Alternative Name Connexin 43 (GJA1) (GJA1 Antibody Abstract)
Background Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.
Synonyms: Gap junction alpha-1 protein, Connexin 43, Cx43, Gap junction 43 kDa heart protein
Molecular Weight 42877 DA
Gene ID 2697
UniProt P17302
Research Area Stem Cells, Signaling, Chromatin, Cell Structure
Pathways MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization

Application Details

Product Details anti-GJA1 Antibody Target Details GJA1 Handling References for anti-GJA1 Antibody (ABIN388369) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only

Handling

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details References for anti-GJA1 Antibody (ABIN388369) Images back to top
Format Liquid
Concentration 1.7 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-GJA1 Antibody (ABIN388369)

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling Images back to top
Product cited in:

Liu, Sun, Torii, Rakic: "Connexin 43 controls the multipolar phase of neuronal migration to the cerebral cortex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 21, pp. 8280-5, 2012

Ponsaerts, De Vuyst, Retamal, Dhondt, Vermeire, Wang, De Smedt, Zimmermann, Himpens, Vereecke, Leybaert, Bultynck: "Intramolecular loop/tail interactions are essential for connexin 43-hemichannel activity." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 11, pp. 4378-95, 2010

Li, Zhang, Jiao, Zou: "Knockdown of microRNA-181 by lentivirus mediated siRNA expression vector decreases the arrhythmogenic effect of skeletal myoblast transplantation in rat with myocardial infarction." in: Microvascular research, Vol. 78, Issue 3, pp. 393-404, 2009

Cina, Maass, Theis, Willecke, Bechberger, Naus: "Involvement of the cytoplasmic C-terminal domain of connexin43 in neuronal migration." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 7, pp. 2009-21, 2009

Background publications

Spinella, Rosanò, Di Castro, Nicotra, Natali, Bagnato: "Endothelin-1 decreases gap junctional intercellular communication by inducing phosphorylation of connexin 43 in human ovarian carcinoma cells." in: The Journal of biological chemistry, Vol. 278, Issue 42, pp. 41294-301, 2003

Contreras, Sáez, Bukauskas, Bennett: "Gating and regulation of connexin 43 (Cx43) hemichannels." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 20, pp. 11388-93, 2003

Qin, Shao, Igdoura, Alaoui-Jamali, Laird: "Lysosomal and proteasomal degradation play distinct roles in the life cycle of Cx43 in gap junctional intercellular communication-deficient and -competent breast tumor cells." in: The Journal of biological chemistry, Vol. 278, Issue 32, pp. 30005-14, 2003

Cameron, Malik, Akaike, Lerner-Marmarosh, Yan, Lee, Abe, Yang: "Regulation of epidermal growth factor-induced connexin 43 gap junction communication by big mitogen-activated protein kinase1/ERK5 but not ERK1/2 kinase activation." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 18682-8, 2003

Ma, Ma, Sui, Wang, Wang: "Signal transduction of gap junctional genes, connexin32, connexin43 in human hepatocarcinogenesis." in: World journal of gastroenterology : WJG, Vol. 9, Issue 5, pp. 946-50, 2003

Images

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling References for anti-GJA1 Antibody (ABIN388369) back to top
Supplier Images
Western Blotting (WB) image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (AA 107-138) (ABIN388369) (LEFT)Western blot analysis of anti-GJA1 Antibody (N-term)(ABIN388369) in CEM cell li...
Immunohistochemistry (IHC) image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (AA 107-138) (ABIN388369) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...