anti-Rat (Rattus) GJA1 antibody for Enzyme Immunoassay

Recommended GJA1 Antibody (supplied by: Log in to see )

Antigen
Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
  • cx43
  • gjal
  • oddd
  • dfnb38
  • connexin43
  • cx43a1
  • gja1
  • zfCx43.3
  • AU042049
  • AW546267
  • Cnx43
  • Cx43
  • Cx43alpha1
  • Gja-1
  • Npm1
  • AVSD3
  • CX43
  • DFNB38
  • GJAL
  • HLHS1
  • HSS
  • ODDD
  • connexin 43
  • gja1-A
  • Cnx46
  • Cx46
  • Gja-3
  • AI118175
  • Cnx32
  • Cx32
  • Gjb-1
  • gap junction protein, alpha 1, 43kDa
  • connexin 43
  • CONNEXIN 43 protein
  • connexin 43-like
  • gap junction protein, alpha 1
  • gap junction protein, alpha 3
  • gap junction protein, beta 1
  • gja1
  • cx43
  • CX43
  • CONNEXIN 43
  • LOC100359132
  • Gja1
  • GJA1
  • Gja3
  • Gjb1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
272
191
173
53
22
22
21
17
16
16
10
10
7
7
6
5
5
4
4
3
3
3
3
2
1
1
1
1
Host
Rabbit
261
34
22
Clonality
Polyclonal
Conjugate
This GJA1 antibody is un-conjugated
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
242
119
102
81
44
20
20
14
13
8
7
5
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN265368
$ 390.50
Plus shipping costs $45.00

General

Antigen Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(272), (191), (173), (53), (22), (22), (21), (17), (16), (16), (10), (10), (7), (7), (6), (5), (5), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(261), (34), (22)
Clonality
Polyclonal
Conjugate This GJA1 antibody is un-conjugated
(5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(242), (119), (102), (81), (44), (20), (20), (14), (13), (8), (7), (5), (2), (1), (1)
Supplier Log in to see

Product Details anti-GJA1 Antibody

Target Details GJA1 Application Details Handling Images
Specificity This antibody detects endogenous levels of GJA1 / Cx43 protein(region surrounding Ser369).
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography using epitope-specific immunogen.

Target Details GJA1

Product Details anti-GJA1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name GJA1 / Cx43 (GJA1 Antibody Abstract)
Background Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication.Synonyms: Connexin-43, GJAL, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein
Molecular Weight approx. 43 kDa
Gene ID 2697
NCBI Accession NP_000156
UniProt P17302
Pathways MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization

Application Details

Product Details anti-GJA1 Antibody Target Details GJA1 Handling Images back to top
Application Notes ELISA: 1/10000approx. 1/20000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid Repeated freeze/thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling back to top
Supplier Images
 image for anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) antibody (ABIN265368) anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) antibody
 image for anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) antibody (ABIN265368) anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) antibody (Image 2)