anti-Zebrafish (Danio rerio) GJA1 antibody for Western Blotting

Recommended GJA1 Antibody (supplied by: Log in to see )

Antigen
Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
  • cx43
  • gjal
  • oddd
  • dfnb38
  • connexin43
  • cx43a1
  • gja1
  • zfCx43.3
  • AU042049
  • AW546267
  • Cnx43
  • Cx43
  • Cx43alpha1
  • Gja-1
  • Npm1
  • AVSD3
  • CX43
  • DFNB38
  • GJAL
  • HLHS1
  • HSS
  • ODDD
  • connexin 43
  • gja1-A
  • Cnx46
  • Cx46
  • Gja-3
  • AI118175
  • Cnx32
  • Cx32
  • Gjb-1
  • gap junction protein, alpha 1, 43kDa
  • connexin 43
  • CONNEXIN 43 protein
  • connexin 43-like
  • gap junction protein, alpha 1
  • gap junction protein, alpha 3
  • gap junction protein, beta 1
  • gja1
  • cx43
  • CX43
  • CONNEXIN 43
  • LOC100359132
  • Gja1
  • GJA1
  • Gja3
  • Gjb1
Epitope
C-Term
68
21
20
18
15
13
10
8
8
7
6
5
5
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Bird (Avian), Dog (Canine), Chicken, Human, Monkey, Mouse (Murine), Rat (Rattus), Sheep (Ovine), Xenopus laevis, Zebrafish (Danio rerio)
281
191
173
52
21
21
21
17
16
16
10
9
7
6
5
5
4
4
4
3
3
3
3
2
1
1
1
1
Host
Rabbit
261
43
22
Clonality
Polyclonal
Conjugate
This GJA1 antibody is un-conjugated
5
5
5
4
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
Application
Western Blotting (WB)
242
119
102
82
45
29
20
14
13
8
7
5
2
2
2
Supplier
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Catalog No. ABIN318733
$ 654.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5551527 WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN319138 WB Rabbit IgG pSer368 Log in to see Polyclonal 3
1 ABIN5551528 WB Rabbit IgG pSer368 Log in to see Polyclonal
1 ABIN644019 WB Rabbit IgG C-Term Log in to see

General

Antigen Gap Junction Protein, alpha 1, 43kDa (GJA1) Antibodies
Epitope C-Term
(68), (21), (20), (18), (15), (13), (10), (8), (8), (7), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bird (Avian), Dog (Canine), Chicken, Human, Monkey, Mouse (Murine), Rat (Rattus), Sheep (Ovine), Xenopus laevis, Zebrafish (Danio rerio)
(281), (191), (173), (52), (21), (21), (21), (17), (16), (16), (10), (9), (7), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(261), (43), (22)
Clonality
Polyclonal
Conjugate This GJA1 antibody is un-conjugated
(5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(242), (119), (102), (82), (45), (29), (20), (14), (13), (8), (7), (5), (2), (2), (2)
Pubmed 1 reference available
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Product Details anti-GJA1 Antibody

Target Details GJA1 Application Details Handling References for anti-GJA1 antibody (ABIN318733) Images
Specificity This antibody recognises rat connexin43.
Purification Affinity chromatography
Immunogen Synthetic peptide corresponding to the C-terminal region of rat connexin 43 (CT).
Isotype IgG

Target Details GJA1

Product Details anti-GJA1 Antibody Application Details Handling References for anti-GJA1 antibody (ABIN318733) Images back to top
Antigen
Alternative Name GJA1 / Cx43 (GJA1 Antibody Abstract)
Background Connexin43 is a 43 kDa member of the Alpha-type (group II) subfamily of the connexins. It is a component of gap junctions, the small channels linking the cytoplasm of adjacent cells and enabling signalling through the transfer of ions and metabolites. Phosphorylation of Connexin 43 at serine 368 regulates the permeability of the gap junction. Connexin 43 is the major protein of gap junctions in the heart, essential to synchronised contractions. It is also crucial in embryonic development and is associated with epilepsy.Synonyms: Connexin-43, GJAL, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein
Gene ID 2697
NCBI Accession NP_000156
UniProt P17302
Pathways MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization

Application Details

Product Details anti-GJA1 Antibody Target Details GJA1 Handling References for anti-GJA1 antibody (ABIN318733) Images back to top
Application Notes Western Blot: 1: 1000, detects a band of approximately 43 kDa in rat cerebellar lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details References for anti-GJA1 antibody (ABIN318733) Images back to top
Format Liquid
Buffer 10 mM Hepes pH 7.5, 150 mM NaCl containing 0.09 % Sodium Azide, 0.01 % Bovine Serum Albumin, 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-GJA1 antibody (ABIN318733)

Product Details anti-GJA1 Antibody Target Details GJA1 Application Details Handling Images back to top
Background publications

Bao, Reuss, Altenberg: "Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368." in: The Journal of biological chemistry, Vol. 279, Issue 19, pp. 20058-66, 2004