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anti-Chicken HSPB1 antibody for Immunoprecipitation

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • Hs.78846
  • LOH11CR1K
  • MKBP
  • Hsp27
  • 27kDa
  • 2810021G24Rik
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock 27kDa protein 1
  • heat shock 27kDa protein 2
  • heat shock protein 1
  • heat shock protein 2
  • heat shock protein 25
  • hspb1
  • HSPB1
  • HSP27
  • HSPB2
  • Hspb1
  • Hspb2
  • HSP25
Reactivity
Chicken, Human, Mouse (Murine), Primate, Rat (Rattus), Sheep (Ovine)
838
289
284
87
80
78
70
57
53
38
32
30
19
13
11
9
6
4
4
2
2
2
2
2
1
1
Host
Mouse
511
349
3
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
34
34
23
20
13
13
13
13
12
11
10
10
10
9
9
9
9
9
6
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
725
346
284
250
228
213
173
123
60
40
18
15
9
8
6
4
2
2
2
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Catalog No. ABIN4320024
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4320034 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320041 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320045 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320035 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320037 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320043 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320038 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320051 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320050 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320055 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320032 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320053 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320025 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320031 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320036 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320040 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320042 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320046 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320048 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320054 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 kappa Log in to see SPM252

Similar anti-HSPB1 Antibodies

Application / Reactivity Sheep (Ovine) Rat (Rattus)
Cytometry by Time of Flight (CyTOF) 6 Antibodies 8 Antibodies
ELISA 32 Antibodies 60 Antibodies
Flow Cytometry (FACS) 70 Antibodies 103 Antibodies
Immunocytochemistry (ICC) 38 Antibodies 105 Antibodies
Immunofluorescence (IF) 52 Antibodies 132 Antibodies
Immunohistochemistry (IHC) 6 Antibodies 88 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 35 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 65 Antibodies
Immunoprecipitation (IP) 33 Antibodies
Western Blotting (WB) 67 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Chicken, Human, Mouse (Murine), Primate, Rat (Rattus), Sheep (Ovine)
(838), (289), (284), (87), (80), (78), (70), (57), (53), (38), (32), (30), (19), (13), (11), (9), (6), (4), (4), (2), (2), (2), (2), (2), (1), (1)
Host Mouse
(511), (349), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(34), (34), (23), (20), (13), (13), (13), (13), (12), (11), (10), (10), (10), (9), (9), (9), (9), (9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(725), (346), (284), (250), (228), (213), (173), (123), (60), (40), (18), (15), (9), (8), (6), (4), (2), (2), (2)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Specificity This MAb reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosophila HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumors.
Purification Protein A purified
Immunogen Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
Clone G3-1
Isotype IgG1 kappa

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background Gene Symbol: HSPB1
Molecular Weight Theoretical MW: 27 kDa
Gene ID 3315
UniProt P04792
Research Area Heat Shock Proteins
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes Western Blot 0.25-0.5 μg/mL, Flow Cytometry 0.5-1 μg/million cells, Immunocytochemistry/Immunofluorescence 0.5-1 μg/mL, Immunoprecipitation 0.5-1 μg/500 μg protein lysate, Immunohistochemistry-Paraffin 0.5-1.0 μg/mL, Immunohistochemistry-Frozen 0.5-1.0 μg/mLELISA (For coating, order NBP2-34621 (BSA free version of NBP2-32972))
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Format Liquid
Buffer PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.