anti-Human HSPB1 antibody for Flow Cytometry

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • Hs.78846
  • LOH11CR1K
  • MKBP
  • Hsp27
  • 27kDa
  • 2810021G24Rik
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock 27kDa protein 1
  • heat shock 27kDa protein 2
  • heat shock protein 1
  • heat shock protein 2
  • heat shock protein 25
  • hspb1
  • HSPB1
  • HSP27
  • HSPB2
  • Hspb1
  • Hspb2
  • HSP25
Reactivity
Human, Rat (Rattus)
810
283
276
96
87
72
71
69
46
31
30
26
18
13
11
9
6
4
3
2
2
2
1
1
1
1
Host
Mouse
490
339
3
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
34
34
23
20
13
13
13
13
12
11
10
10
10
9
9
9
9
9
6
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
699
325
270
252
221
207
168
156
61
40
17
11
11
8
6
4
2
2
1
1
1
Options
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Catalog No. ABIN969199
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.326658 ABIN4320008 CyTOF FACS ICC IF WB Mouse IgG1 Log in to see 6H11
14.326658 ABIN1996522 FACS FITC Rabbit IgG AA 1-205 Log in to see 155
14.326658 ABIN1996524 FACS PE Rabbit IgG AA 1-205 Log in to see 155
14.326658 ABIN4319976 ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 5D7
14.326658 ABIN2214037 FACS FITC Mouse IgG1 AA 1-205 Log in to see 09
14.326658 ABIN1574090 FACS ELISA WB Rabbit IgG AA 180-230 Log in to see Polyclonal
14.326658 ABIN1845754 ELISA FACS IF/ICC IHC WB Mouse IgG1 Log in to see
1 ABIN361756 FACS ICC IF IHC IP WB Mouse IgG1 kappa Log in to see 8A7 9
1 ABIN1027725 FACS ICC IF WB Rabbit Log in to see Polyclonal 7
1 ABIN863100 FACS FITC Mouse IgG2b kappa Log in to see 5D12-A12 6
1 ABIN2854951 ICC FACS IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN2484354 FACS ICC IF WB APC Rabbit Log in to see Polyclonal 6
1 ABIN2484345 FACS ICC IF WB Atto 390 Rabbit Log in to see Polyclonal 6
1 ABIN2484346 FACS ICC IF WB Atto 488 Rabbit Log in to see Polyclonal 6
1 ABIN2484347 FACS ICC IF WB Atto 565 Rabbit Log in to see Polyclonal 6
1 ABIN2484348 FACS ICC IF WB Atto 594 Rabbit Log in to see Polyclonal 6
1 ABIN2484349 FACS ICC IF WB Atto 633 Rabbit Log in to see Polyclonal 6
1 ABIN2484350 FACS ICC IF WB Atto 655 Rabbit Log in to see Polyclonal 6
1 ABIN2484351 FACS ICC IF WB Atto 680 Rabbit Log in to see Polyclonal 6
1 ABIN2484352 FACS ICC IF WB Atto 700 Rabbit Log in to see Polyclonal 6

Top referenced anti-HSPB1 antibody for Flow Cytometry

Similar anti-HSPB1 Antibodies

Application / Reactivity Rat (Rattus) Human
Cytometry by Time of Flight (CyTOF) 8 Antibodies 8 Antibodies
ELISA 59 Antibodies 259 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 119 Antibodies
Immunocytochemistry (ICC) 102 Antibodies
Immunofluorescence (IF) 128 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 82 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 45 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 115 Antibodies
Immunoprecipitation (IP) 59 Antibodies
Western Blotting (WB) 223 Antibodies
Cell Culture (CC) 1 Antibodies
Dot Blot (DB) 17 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Human, Rat (Rattus)
(810), (283), (276), (96), (87), (72), (71), (69), (46), (31), (30), (26), (18), (13), (11), (9), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(490), (339), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(34), (34), (23), (20), (13), (13), (13), (13), (12), (11), (10), (10), (10), (9), (9), (9), (9), (9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(699), (325), (270), (252), (221), (207), (168), (156), (61), (40), (17), (11), (11), (8), (6), (4), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling ProductDetails: References for anti-HSPB1 antibody (ABIN969199) Images
Purification Ascites
Immunogen Purified recombinant fragment of human HSP27 expressed in E. Coli.
Clone 5D7
Isotype IgG1

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling ProductDetails: References for anti-HSPB1 antibody (ABIN969199) Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). (provided by RefSeq) Tissue specificity: Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Molecular Weight 27 kDa
Gene ID 3315
HGNC 3315
Research Area Heat Shock Proteins
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling ProductDetails: References for anti-HSPB1 antibody (ABIN969199) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details ProductDetails: References for anti-HSPB1 antibody (ABIN969199) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

ProductDetails: References for anti-HSPB1 antibody (ABIN969199)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Product cited in:

Langer, Ott, Specht, Becker, Lordick, Burian, Herrmann, Schrattenholz, Cahill, Schwaiger, Hofler, Wester: "Protein expression profiling in esophageal adenocarcinoma patients indicates association of heat-shock protein 27 expression and chemotherapy response." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 24, pp. 8279-87, 2008

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling ProductDetails: References for anti-HSPB1 antibody (ABIN969199) back to top
Supplier Images
ELISA image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 2: Flow cytometric analysis of HepG2 cells using HSP27 mouse mAb (green) and n...
Immunocytochemistry (ICC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 3: Immunofluorescence analysis of Hela cells using HSP27 mouse mAb (green) . B...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 4: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and ...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 5: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (le...
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 6: Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jur...