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anti-Human HSPB1 antibody for Flow Cytometry

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Reactivity
Human, Rat (Rattus)
751
272
259
86
79
76
73
53
41
38
24
22
18
8
4
4
3
2
1
1
1
Host
Mouse
480
310
3
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
32
31
23
17
13
13
11
10
10
9
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
667
288
265
238
229
187
184
127
68
34
18
15
5
2
2
2
Supplier
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Catalog No. ABIN969199
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30464333 ABIN492422 FACS FITC Mouse IgG2b Log in to see 5D12-A12
0.30464333 ABIN492423 FACS FITC Mouse IgG2b Log in to see 5D12-A12
0.30464333 ABIN1574090 FACS ELISA WB Rabbit IgG AA 180-230 Log in to see Polyclonal
0.30464333 ABIN1996522 FACS FITC Rabbit IgG AA 1-205 Log in to see 155
0.30464333 ABIN1996524 FACS PE Rabbit IgG AA 1-205 Log in to see
0.30464333 ABIN1845754 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see
0.30464333 ABIN2214037 FACS FITC Mouse Log in to see
0.0010463598 ABIN863100 FACS FITC Mouse Log in to see 5D12-A12 6
0.0010463598 ABIN2854951 ICC FACS IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.0010463598 ABIN1498733 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see 5D7
0.0010463598 ABIN966308 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 5D7 2
0.0010463598 ABIN470574 FACS FITC Mouse IgG1 Log in to see
0.0010463598 ABIN1838672 FACS ELISA WB Rabbit AA 180-230 Log in to see Polyclonal
0.0010463598 ABIN1102182 FACS FITC Mouse IgG2b, kappa Log in to see 5D12-A12
0.0010463598 ABIN1996519 FACS IF Rabbit IgG AA 1-205 Log in to see
0.0010463598 ABIN865415 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 5D7
0.0010463598 ABIN2286226 FACS IF IP IHC WB Mouse IgG1 kappa Log in to see G3-1
0.0010463598 ABIN2286205 FACS FITC Mouse IgG2b kappa Internal Region Log in to see 5D12-A12
0.0010463598 ABIN4320066 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see HSPB1-774
0.0010463598 ABIN4320067 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see HSPB1-774

Top referenced anti-HSPB1 antibody for Flow Cytometry

  • Human Monoclonal HSPB1 Primary Antibody for FACS, IF - ABIN966308 (2 Publications): Langer, Ott, Specht, Becker, Lordick, Burian, Herrmann, Schrattenholz, Cahill, Schwaiger, Hofler, Wester: Protein expression profiling in esophageal adenocarcinoma patients indicates association of heat-shock protein 27 expression and chemotherapy response. in Clinical cancer research : an official journal of the American Association for Cancer Research 2008 (PubMed)

  • Human Monoclonal HSPB1 Primary Antibody for FACS - ABIN863100 (6 Publications): Ehrnsperger, Gräber, Gaestel, Buchner: Binding of non-native protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation. in The EMBO journal 1997 (PubMed)

Similar anti-HSPB1 Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 42 Antibodies 255 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 15 Antibodies
Flow Cytometry (FACS) 101 Antibodies 123 Antibodies
Immunocytochemistry (ICC) 123 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies
Immunofluorescence (IF) 145 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 90 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 49 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 125 Antibodies
Immunoprecipitation (IP) 82 Antibodies
Western Blotting (WB) 225 Antibodies
Dot Blot (DB) 19 Antibodies
Immunochromatography (IC) 4 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Human, Rat (Rattus)
(751), (272), (259), (86), (79), (76), (73), (53), (41), (38), (24), (22), (18), (8), (4), (4), (3), (2), (1), (1), (1)
Host Mouse
(480), (310), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(32), (31), (23), (17), (13), (13), (11), (10), (10), (9), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(667), (288), (265), (238), (229), (187), (184), (127), (68), (34), (18), (15), (5), (2), (2), (2)
Pubmed 1 reference available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN969199) Images
Purification Ascites
Immunogen Purified recombinant fragment of human HSP27 expressed in E. coli.
Clone 5D7
Isotype IgG1

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 Antibody (ABIN969199) Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). (provided by RefSeq) Tissue specificity: Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
Molecular Weight 27 kDa
Gene ID 3315
HGNC 3315
Research Area Heat Shock Proteins
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 Antibody (ABIN969199) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 Antibody (ABIN969199) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-HSPB1 Antibody (ABIN969199)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Product cited in:

Langer, Ott, Specht, Becker, Lordick, Burian, Herrmann, Schrattenholz, Cahill, Schwaiger, Hofler, Wester: "Protein expression profiling in esophageal adenocarcinoma patients indicates association of heat-shock protein 27 expression and chemotherapy response." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 24, pp. 8279-87, 2008

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN969199) back to top
Supplier Images
ELISA image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 2: Flow cytometric analysis of HepG2 cells using HSP27 mouse mAb (green) and n...
Immunocytochemistry (ICC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 3: Immunofluorescence analysis of Hela cells using HSP27 mouse mAb (green) . B...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 4: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and ...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 5: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (le...
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN969199) Figure 6: Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jur...