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anti-Human HSPB1 antibody for Immunohistochemistry (Frozen Sections)

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • Hs.78846
  • LOH11CR1K
  • MKBP
  • Hsp27
  • 27kDa
  • 2810021G24Rik
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock 27kDa protein 1
  • heat shock 27kDa protein 2
  • heat shock protein 1
  • heat shock protein 2
  • heat shock protein 25
  • hspb1
  • HSPB1
  • HSP27
  • HSPB2
  • Hspb1
  • Hspb2
  • HSP25
Epitope
AA 1-205
78
64
51
44
24
17
16
16
15
13
12
9
6
5
5
5
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
806
281
276
87
78
72
71
51
46
31
30
26
18
13
11
9
6
4
3
2
2
2
1
1
1
1
Host
Rabbit
485
340
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
34
34
23
20
13
13
13
13
12
11
10
10
10
9
9
9
9
9
6
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
695
325
271
250
221
208
168
121
60
40
17
14
11
8
6
4
2
2
1
1
Options
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Catalog No. ABIN3042464
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN126812 IHC (fro) WB Mouse IgG2a pSer82 Log in to see 5B9
1 ABIN3044184 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 88-105, Middle Region Log in to see Polyclonal
1 ABIN126811 IHC (fro) WB Biotin Mouse IgG2a pSer82 Log in to see 5B9
1 ABIN263952 IHC (fro) IF IP WB Mouse IgG1 Log in to see 8A7
1 ABIN1107615 IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see SJ-25
1 ABIN3043648 ICC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see IAP-9
1 ABIN187604 IHC (fro) IHC (p) WB Mouse IgG1 Log in to see G3-1
1 ABIN4320051 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320046 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320058 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320057 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320035 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see G3-1
1 ABIN4320042 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320047 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320055 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320054 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 kappa Log in to see SPM252
1 ABIN4320060 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see G3-1
1 ABIN2622151 ICC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see SJ-25
1 ABIN2622152 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 88-105 Log in to see Polyclonal
1 ABIN2622164 IHC (fro) IHC (p) WB Rabbit IgG AA 1-205 Log in to see Polyclonal

Similar anti-HSPB1 Antibodies

Application / Reactivity Human
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 8 Antibodies
Dot Blot (DB) 17 Antibodies
ELISA 259 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 14 Antibodies
Flow Cytometry (FACS) 121 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 202 Antibodies
Immunofluorescence (IF) 214 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 318 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 61 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope AA 1-205
(78), (64), (51), (44), (24), (17), (16), (16), (15), (13), (12), (9), (6), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(806), (281), (276), (87), (78), (72), (71), (51), (46), (31), (30), (26), (18), (13), (11), (9), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(485), (340), (3)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(34), (34), (23), (20), (13), (13), (13), (13), (12), (11), (10), (10), (10), (9), (9), (9), (9), (9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(695), (325), (271), (250), (221), (208), (168), (121), (60), (40), (17), (14), (11), (8), (6), (4), (2), (2), (1), (1)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Heat shock protein beta-1(HSPB1) detection. Tested with WB, IHC-P, IHC-F in Human.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Heat shock protein beta-1(HSPB1) detection. Tested with WB, IHC-P, IHC-F in Human.
Gene Name: heat shock 27 kDa protein 1
Protein Name: Heat shock protein beta-1
Purification Immunogen affinity purified.
Immunogen E.coli-derived human Hsp27 recombinant protein (Position: M1-K205). Human Hsp27 shares 83% amino acid (aa) sequence identity with mouse Hsp27.
Isotype IgG

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSPB1 (HSPB1 Antibody Abstract)
Background HSPB1(Heat shock 27 kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. HSP27 gene is mapped to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

Synonyms: Heat shock 27 kDa protein antibody|28 kDa heat shock protein antibody|CMT2F antibody|DKFZp586P1322 antibody|Estrogen regulated 24 kDa protein antibody|Estrogen-regulated 24 kDa protein antibody|Heat shock 25 kDa protein 1 antibody|Heat shock 25 kDa protein 1 antibody|Heat shock 27 kDa protein antibody|Heat shock 27kD protein 1 antibody|Heat shock 27 kDa protein 1 antibody|Heat shock 27 kDa protein 1 antibody|Heat shock 28 kDa protein 1 antibody|Heat shock 28 kDa protein 1 antibody|Heat Shock Protein 27 antibody|Heat Shock Protein 27 antibody|Heat shock protein beta 1 antibody|Heat shock protein beta-1 antibody|Heat Shock Protein27 antibody|Heat Shock Protein27 antibody|HMN2B antibody|HS.76067 antibody|Hsp 25 antibody|Hsp 25 antibody|Hsp 27 antibody|Hsp 27 antibody|Hsp 28 antibody|Hsp 28 antibody|Hsp B1 antibody|Hsp B1 antibody|Hsp25 antibody|Hsp25 antibody|HSP27 antibody|Hsp28 antibody|Hsp28 antibody|HspB1 antibody|HspB1 antibody|HSPB1_HUMAN antibody|SRP27 antibody|Stress responsive protein 27 antibody|Stress-responsive protein 27 antibody
Gene ID 3315
UniProt P04792
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Hsp27 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 1-205) (ABIN3042464) Anti- HSP27 antibody, IHC(P) IHC(P): Human Intestinal Cancer Tissue
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 1-205) (ABIN3042464) Anti- HSP27 antibody, Western blotting All lanes: Anti HSP27 at 0.5ug/ml WB: Recombi...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 1-205) (ABIN3042464) Anti- HSP27 antibody, IHC(F) IHC(F): Human Placenta Tissue
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 1-205) (ABIN3042464) Anti- HSP27 antibody, IHC(P) IHC(P): Human Mammary Cancer Tissue
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 1-205) (ABIN3042464) Anti- HSP27 antibody, Western blotting All lanes: Anti HSP27 at 0.5ug/ml Lane 1: NRK...