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|Application / Reactivity||Human|
|Cell Culture (CC)||1 Antibodies|
|Cytometry by Time of Flight (CyTOF)||8 Antibodies|
|Dot Blot (DB)||17 Antibodies|
|ELISA (Detection)||1 Antibodies|
|Enzyme Immunoassay (EIA)||14 Antibodies|
|Flow Cytometry (FACS)||121 Antibodies|
|Immunochromatography (IC)||5 Antibodies|
|Immunocytochemistry (ICC)||202 Antibodies|
|Immunofluorescence (IF)||214 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||40 Antibodies|
|Immunohistochemistry (IHC)||318 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||61 Antibodies|
|Antigen||Heat Shock 27kDa Protein 1 (HSPB1) Antibodies|
|Epitope||AA 1-205 Alternatives|
|Conjugate||This HSPB1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-HSPB1 AntibodyTarget Details HSPB1 Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Heat shock protein beta-1(HSPB1) detection. Tested with WB, IHC-P, IHC-F in Human.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Heat shock protein beta-1(HSPB1) detection. Tested with WB, IHC-P, IHC-F in Human.
Gene Name: heat shock 27 kDa protein 1
Protein Name: Heat shock protein beta-1
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human Hsp27 recombinant protein (Position: M1-K205). Human Hsp27 shares 83% amino acid (aa) sequence identity with mouse Hsp27.|
Target Details HSPB1Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
|Alternative Name||HSPB1 (HSPB1 Antibody Abstract)|
HSPB1(Heat shock 27 kDa protein 1), also known as HSP27, is a protein that in humans is encoded by the HSPB1 gene. HSP27 gene is mapped to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
Synonyms: Heat shock 27 kDa protein antibody|28 kDa heat shock protein antibody|CMT2F antibody|DKFZp586P1322 antibody|Estrogen regulated 24 kDa protein antibody|Estrogen-regulated 24 kDa protein antibody|Heat shock 25 kDa protein 1 antibody|Heat shock 25 kDa protein 1 antibody|Heat shock 27 kDa protein antibody|Heat shock 27kD protein 1 antibody|Heat shock 27 kDa protein 1 antibody|Heat shock 27 kDa protein 1 antibody|Heat shock 28 kDa protein 1 antibody|Heat shock 28 kDa protein 1 antibody|Heat Shock Protein 27 antibody|Heat Shock Protein 27 antibody|Heat shock protein beta 1 antibody|Heat shock protein beta-1 antibody|Heat Shock Protein27 antibody|Heat Shock Protein27 antibody|HMN2B antibody|HS.76067 antibody|Hsp 25 antibody|Hsp 25 antibody|Hsp 27 antibody|Hsp 27 antibody|Hsp 28 antibody|Hsp 28 antibody|Hsp B1 antibody|Hsp B1 antibody|Hsp25 antibody|Hsp25 antibody|HSP27 antibody|Hsp28 antibody|Hsp28 antibody|HspB1 antibody|HspB1 antibody|HSPB1_HUMAN antibody|SRP27 antibody|Stress responsive protein 27 antibody|Stress-responsive protein 27 antibody
|Pathways||MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling|
Application DetailsProduct Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Hsp27 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).
|Restrictions||For Research Use only|
HandlingProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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