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anti-Human HSPB1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • Hs.78846
  • LOH11CR1K
  • MKBP
  • Hsp27
  • 27kDa
  • 2810021G24Rik
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock 27kDa protein 1
  • heat shock 27kDa protein 2
  • heat shock protein 1
  • heat shock protein 2
  • heat shock protein 25
  • hspb1
  • HSPB1
  • HSP27
  • HSPB2
  • Hspb1
  • Hspb2
  • HSP25
Epitope
Ser82
83
68
53
44
24
16
16
16
15
13
12
9
6
5
5
5
5
5
4
4
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
838
290
285
88
80
79
71
57
53
38
32
30
19
13
11
9
6
4
4
2
2
2
2
2
1
1
Host
Rabbit
510
350
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
34
34
23
20
13
13
13
13
12
11
10
10
10
9
9
9
9
9
6
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
725
346
284
250
229
214
174
124
61
40
18
14
9
8
6
4
2
2
2
Supplier
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Catalog No. ABIN4620439
$ 375.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.070733 ABIN4620438 EIA IHC (p) WB Rabbit Ig Ser78 Log in to see Polyclonal 5
13.070733 ABIN672441 IF (p) IHC (p) WB Rabbit IgG AA 100-150 Log in to see Polyclonal 2
13.070733 ABIN4319982 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
13.070733 ABIN316084 IHC (p) IHC ELISA WB Mouse IgG1 AA 1-205 Log in to see 2A5
13.070733 ABIN4319981 IHC IHC (p) Rabbit IgG Log in to see Polyclonal 2
13.070733 ABIN410085 ICC IF IHC (p) IP IHC WB Mouse IgG1 kappa Log in to see 8A7
13.070733 ABIN498848 IHC (p) Rabbit pSer78, pSer82 Log in to see Polyclonal
13.070733 ABIN498746 IHC (p) WB Rabbit pSer82 Log in to see Polyclonal
13.070733 ABIN1107612 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal
13.070733 ABIN1107613 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
13.070733 ABIN498463 IHC (p) WB Rabbit Log in to see Polyclonal
13.070733 ABIN498626 IHC (p) WB Rabbit Log in to see Polyclonal
13.070733 ABIN4319992 ICC IF IHC IHC (p) IP WB Rabbit IgG Center Log in to see Polyclonal
13.070733 ABIN364326 IHC (p) ELISA WB Mouse IgG1 AA 1-205 Log in to see 2A5
13.070733 ABIN214359 ICC IHC (p) IP ELISA WB Mouse IgG1, lambda Log in to see
13.070733 ABIN615155 IHC (p) IP WB Rabbit Log in to see Polyclonal
13.070733 ABIN743138 IF (p) IHC (p) WB Rabbit IgG pSer15 Log in to see Polyclonal
13.070733 ABIN462100 IHC (p) WB Mouse IgG1 Log in to see 5F12-B10
13.070733 ABIN4320006 IHC (p) WB Rabbit Log in to see Polyclonal
13.070733 ABIN498937 IHC (p) Rabbit Log in to see Polyclonal

Top referenced anti-HSPB1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-HSPB1 Antibodies

Application / Reactivity Human
Cytometry by Time of Flight (CyTOF) 8 Antibodies
Dot Blot (DB) 18 Antibodies
ELISA 272 Antibodies
Enzyme Immunoassay (EIA) 15 Antibodies
Flow Cytometry (FACS) 122 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 208 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies
Immunofluorescence (IF) 222 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 339 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 61 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 249 Antibodies
Immunoprecipitation (IP) 165 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope Ser82
(83), (68), (53), (44), (24), (16), (16), (16), (15), (13), (12), (9), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(838), (290), (285), (88), (80), (79), (71), (57), (53), (38), (32), (30), (19), (13), (11), (9), (6), (4), (4), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(510), (350), (3)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(34), (34), (23), (20), (13), (13), (13), (13), (12), (11), (10), (10), (10), (9), (9), (9), (9), (9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(725), (346), (284), (250), (229), (214), (174), (124), (61), (40), (18), (14), (9), (8), (6), (4), (2), (2), (2)
Pubmed 5 references available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN4620439) Images
Specificity This antibody detects HSPB1.
Purification Protein A column, followed by peptide affinity purification
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S82 of human HSPB1.
Isotype Ig

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 Antibody (ABIN4620439) Images back to top
Antigen
Alternative Name HSPB1 / HSP27 (HSPB1 Antibody Abstract)
Background In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSBP1 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores, and high levels are associated with carcinoma of the breast and with endometrial adenocarcinomas. Heat shock of HeLa cell cultures, or treatment with arsenite, phorbol ester, or tumor necrosis factor, causes a rapid phosphorylation of preexisting HSBP1, with Ser82 as the major site and Ser78 the minor site of phosphorylation. HSBP1 may exert phosphorylation-activated functions linked with growth signaling pathways in unstressed cells. A homeostatic function at this level could protect cells from adverse effects of signal transduction systems which may be activated inappropriately during stress.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27
Molecular Weight 22783 Da.
Gene ID 3315, 9606
UniProt P04792
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 Antibody (ABIN4620439) Images back to top
Application Notes ELISA 1: 1,000. Western blot 1: 50 - 1: 100. Immunohistochemistry 1: 10 - 1: 50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 Antibody (ABIN4620439) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-HSPB1 Antibody (ABIN4620439)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Background publications

Wano, Kita, Takahashi, Sugaya, Hino, Hosoya, Suzuki: "Protective role of HSP27 against UVC-induced cell death in human cells." in: Experimental cell research, Vol. 298, Issue 2, pp. 584-92, 2004

Evgrafov, Mersiyanova, Irobi, Van Den Bosch, Dierick, Leung, Schagina, Verpoorten, Van Impe, Fedotov, Dadali, Auer-Grumbach, Windpassinger, Wagner, Mitrovic, Hilton-Jones, Talbot, Martin, Vasserman et al.: "Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. ..." in: Nature genetics, Vol. 36, Issue 6, pp. 602-6, 2004

Song, Ethier, Dziubinski, Lin: "Stat3 modulates heat shock 27kDa protein expression in breast epithelial cells." in: Biochemical and biophysical research communications, Vol. 314, Issue 1, pp. 143-50, 2004

Chauhan, Li, Hideshima, Podar, Mitsiades, Mitsiades, Catley, Tai, Hayashi, Shringarpure, Burger, Munshi, Ohtake, Saxena, Anderson: "Hsp27 inhibits release of mitochondrial protein Smac in multiple myeloma cells and confers dexamethasone resistance." in: Blood, Vol. 102, Issue 9, pp. 3379-86, 2003

Van Why, Mann, Ardito, Thulin, Ferris, Macleod, Kashgarian, Siegel: "Hsp27 associates with actin and limits injury in energy depleted renal epithelia." in: Journal of the American Society of Nephrology : JASN, Vol. 14, Issue 1, pp. 98-106, 2002

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN4620439) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Ser82) (ABIN4620439) Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HSPB1...
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Ser82) (ABIN4620439) Western blot analysis of HSPB1 (arrow) using rabbit polyclonal HSPB1 Antibody. 293 ce...