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anti-Human HSPB1 antibody for Immunoprecipitation

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Epitope
Center
67
66
52
40
25
22
16
16
13
13
12
7
6
5
5
5
5
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
753
273
261
87
79
78
73
54
42
39
24
22
18
8
4
4
4
2
2
1
1
1
Host
Rabbit
480
313
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
32
31
23
17
13
13
11
10
10
9
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
670
291
266
239
230
189
184
127
68
34
18
15
5
2
2
2
Supplier
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Catalog No. ABIN2854951
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.5094592 ABIN516740 IF IP PLA ELISA WB Mouse IgG2a kappa AA 96-205, partial Log in to see 3G3
0.00089556724 ABIN263953 IHC (fro) IF IP WB Mouse IgG1 Log in to see 8A7 8
0.00089556724 ABIN361649 ICC IF IP IHC ELISA WB Mouse Log in to see 5D12-A12 7
0.00089556724 ABIN361756 ICC IF IP IHC WB Mouse Log in to see 8A7 9
0.00089556724 ABIN222927 IF IP WB Rabbit IgG Log in to see Polyclonal 1
0.00089556724 ABIN189426 DB ICC IF IHC (p) IP IHC WB Rabbit IgG Ser78 Log in to see Y175
0.00089556724 ABIN263952 IHC (fro) IF IP WB Mouse IgG1 Log in to see 8A7
0.00089556724 ABIN452670 EIA IHC (p) IP WB Mouse IgG2b Log in to see 5D12-A12
0.00089556724 ABIN452669 EIA IHC (p) IP WB Mouse IgG2b Log in to see 5D12-A12
0.00089556724 ABIN1102201 IHC (p) IP ELISA WB Mouse IgG2b, kappa Log in to see 5D12-A12
0.00089556724 ABIN400642 EIA IF IHC (p) IP WB Mouse IgG1 Log in to see n-a
0.00089556724 ABIN100223 IP IHC ELISA WB Mouse IgG Log in to see G3-1
0.00089556724 ABIN2706342 IP IHC WB Rabbit Center Log in to see Polyclonal
0.00089556724 ABIN2707582 IP IHC WB Rabbit N-Term, pSer15 Log in to see Polyclonal
0.00089556724 ABIN1996515 IP ELISA WB Mouse IgG2b AA 1-205 Log in to see 4E 8H3
0.00089556724 ABIN220301 IP WB Rabbit IgG pSer78 Log in to see Polyclonal
0.00089556724 ABIN331272 IP ELISA WB Mouse IgG1, kappa pSer82 Log in to see
0.00089556724 ABIN127002 IF IP WB Rabbit Log in to see Polyclonal
0.00089556724 ABIN215210 ICC IP IHC ELISA WB Biotin Mouse IgG1 Log in to see
0.00089556724 ABIN615155 IHC (p) IP WB Rabbit Log in to see Polyclonal

Top referenced anti-HSPB1 antibody for Immunoprecipitation

Similar anti-HSPB1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 42 Antibodies 39 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 2 Antibodies
Flow Cytometry (FACS) 101 Antibodies 99 Antibodies
Immunocytochemistry (ICC) 124 Antibodies 136 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies 2 Antibodies
Immunofluorescence (IF) 146 Antibodies 156 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 91 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 49 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 126 Antibodies
Immunoprecipitation (IP) 82 Antibodies
Western Blotting (WB) 226 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope Center
(67), (66), (52), (40), (25), (22), (16), (16), (13), (13), (12), (7), (6), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(753), (273), (261), (87), (79), (78), (73), (54), (42), (39), (24), (22), (18), (8), (4), (4), (4), (2), (2), (1), (1), (1)
Host Rabbit
(480), (313), (3)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(32), (31), (23), (17), (13), (13), (11), (10), (10), (9), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(670), (291), (266), (239), (230), (189), (184), (127), (68), (34), (18), (15), (5), (2), (2), (2)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (100 %)
Characteristics Rabbit Polyclonal antibody to HSP27 (heat shock 27 kDa protein 1)
HSP27 antibody
Purification Affinity purified by Protein A.
Immunogen Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.
Isotype IgG

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

Cellular Localization: Cytoplasm , Nucleus , Spindle
Molecular Weight 23 kDa
Gene ID 3315
Research Area Heat Shock Proteins
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes Suggested dilution Reference Flow cytometry/FACS Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACSAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:1000* Western blot1:500-1:10000*
Comment

Positive Control: A431 , HeLa , mouse liver , *HT29

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Format Liquid
Concentration 3.23 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Center) (ABIN2854951) WB Image Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE antibody dil...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Center) (ABIN2854951) IHC-P Image HSP27 antibody detects HSPB1 protein at cytosol on U87 xenograft by immun...
Immunoprecipitation (IP) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Center) (ABIN2854951) IP Image Immunoprecipitation of HSP27 protein from HeLa whole cell extracts using 5 μ...
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Center) (ABIN2854951) WB Image HSP27 antibody detects HSP27 protein by western blot analysis. A. 30 μg A431...
Immunofluorescence (IF) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Center) (ABIN2854951) ICC/IF Image HSP27 antibody detects HSP27 protein at cytoplasm and cytoskeleton by im...