anti-Human HSPB1 antibody for Immunoprecipitation

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • Hs.78846
  • LOH11CR1K
  • MKBP
  • Hsp27
  • 27kDa
  • 2810021G24Rik
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock 27kDa protein 1
  • heat shock 27kDa protein 2
  • heat shock protein 1
  • heat shock protein 2
  • heat shock protein 25
  • hspb1
  • HSPB1
  • HSP27
  • HSPB2
  • Hspb1
  • Hspb2
  • HSP25
Epitope
Center
80
67
54
45
24
16
15
15
15
13
12
8
6
5
5
5
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
812
278
273
96
85
71
68
66
36
30
29
26
18
11
11
9
6
3
2
2
2
2
1
1
1
Host
Rabbit
498
331
2
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
33
31
22
20
13
13
13
13
11
11
10
10
10
9
9
9
9
9
6
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
701
323
270
257
222
206
164
155
59
40
17
11
11
8
6
4
2
2
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2854951
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
23.265068 ABIN4319992 ICC IF IHC IHC (p) IP WB Rabbit IgG Center Log in to see Polyclonal
23.265068 ABIN2481411 FACS ICC IF IHC IP WB Atto 390 Mouse IgG1 kappa Log in to see 8A7
23.265068 ABIN2481412 FACS ICC IF IHC IP WB Atto 488 Mouse IgG1 kappa Log in to see 8A7
23.265068 ABIN2481413 FACS ICC IF IHC IP WB Atto 565 Mouse IgG1 kappa Log in to see 8A7
23.265068 ABIN2481414 FACS ICC IF IHC IP WB Atto 594 Mouse IgG1 kappa Log in to see 8A7
23.265068 ABIN2481415 FACS ICC IF IHC IP WB Atto 633 Mouse IgG1 kappa Log in to see 8A7
23.265068 ABIN2481416 FACS ICC IF IHC IP WB Atto 655 Mouse IgG1 kappa Log in to see 8A7
23.265068 ABIN2481417 FACS ICC IF IHC IP WB Atto 680 Mouse IgG1 kappa Log in to see 8A7
23.265068 ABIN2481418 FACS ICC IF IHC IP WB Atto 700 Mouse IgG1 kappa Log in to see 8A7
23.265068 ABIN2481419 FACS ICC IF IHC IP WB Alkaline Phosphatase (AP) Mouse IgG1 kappa Log in to see 8A7
23.265068 ABIN2481421 FACS ICC IF IHC IP WB Biotin Mouse IgG1 kappa Log in to see 8A7
23.265068 ABIN2481422 FACS ICC IF IHC IP WB FITC Mouse IgG1 kappa Log in to see 8A7
23.265068 ABIN2481423 FACS ICC IF IHC IP WB HRP Mouse IgG1 kappa Log in to see 8A7
23.265068 ABIN2481424 FACS ICC IF IHC IP WB PE-Atto 594 Mouse IgG1 kappa Log in to see 8A7
23.265068 ABIN2481425 FACS ICC IF IHC IP WB PerCP Mouse IgG1 kappa Log in to see 8A7
23.265068 ABIN2481426 FACS ICC IF IHC IP WB PE Mouse IgG1 kappa Log in to see 8A7
23.265068 ABIN2481427 FACS ICC IF IHC IP WB Streptavidin Mouse IgG1 kappa Log in to see 8A7
23.265068 ABIN2481420 FACS ICC IF IHC IP WB APC Mouse IgG1 kappa Log in to see 8A7
23.265068 ABIN516740 IF IP PLA ELISA WB Mouse IgG2a kappa AA 96-205, partial Log in to see 3G3
1 ABIN361756 FACS ICC IF IHC IP WB Mouse IgG1 kappa Log in to see 8A7 9

Top referenced anti-HSPB1 antibody for Immunoprecipitation

Similar anti-HSPB1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 8 Antibodies 8 Antibodies
ELISA 58 Antibodies 54 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 1 Antibodies
Flow Cytometry (FACS) 119 Antibodies 129 Antibodies
Immunocytochemistry (ICC) 102 Antibodies
Immunofluorescence (IF) 127 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 80 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 45 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 115 Antibodies
Immunoprecipitation (IP) 59 Antibodies
Western Blotting (WB) 220 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope Center
(80), (67), (54), (45), (24), (16), (15), (15), (15), (13), (12), (8), (6), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(812), (278), (273), (96), (85), (71), (68), (66), (36), (30), (29), (26), (18), (11), (11), (9), (6), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(498), (331), (2)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(33), (31), (22), (20), (13), (13), (13), (13), (11), (11), (10), (10), (10), (9), (9), (9), (9), (9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(701), (323), (270), (257), (222), (206), (164), (155), (59), (40), (17), (11), (11), (8), (6), (4), (2), (2), (1), (1), (1)
Supplier Log in to see

Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (100 %)
Characteristics Rabbit Polyclonal antibody to HSP27 (heat shock 27 kDa protein 1)
HSP27 antibody
Purification Affinity purified by Protein A.
Immunogen Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.
Isotype IgG

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

Cellular Localization: Cytoplasm , Nucleus , Spindle
Molecular Weight 23 kDa
Gene ID 3315
Research Area Heat Shock Proteins
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes Suggested dilution Reference Flow cytometry/FACS Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACSAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:1000* Western blot1:500-1:10000*
Comment

Positive Control: A431 , HeLa , mouse liver , *HT29

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Format Liquid
Concentration 3.23 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Center) (ABIN2854951) WB Image Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE antibody dil...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Center) (ABIN2854951) IHC-P Image HSP27 antibody detects HSPB1 protein at cytosol on U87 xenograft by immun...
Immunoprecipitation (IP) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Center) (ABIN2854951) IP Image Immunoprecipitation of HSP27 protein from HeLa whole cell extracts using 5 μ...
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Center) (ABIN2854951) WB Image HSP27 antibody detects HSP27 protein by western blot analysis. A. 30 μg A431...
Immunofluorescence (IF) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Center) (ABIN2854951) ICC/IF Image HSP27 antibody detects HSP27 protein at cytoplasm and cytoskeleton by im...