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anti-Human HSPB1 antibody for Western Blotting

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • Hs.78846
  • LOH11CR1K
  • MKBP
  • Hsp27
  • 27kDa
  • 2810021G24Rik
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock 27kDa protein 1
  • heat shock 27kDa protein 2
  • heat shock protein 1
  • heat shock protein 2
  • heat shock protein 25
  • hspb1
  • HSPB1
  • HSP27
  • HSPB2
  • Hspb1
  • Hspb2
  • HSP25
Epitope
AA 108-136, Center
78
64
51
45
24
17
16
16
15
13
12
8
6
5
5
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
806
281
276
87
78
72
71
51
46
31
30
26
18
13
11
9
6
4
3
2
2
2
1
1
1
1
Host
Rabbit
485
340
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
34
34
23
20
13
13
13
13
12
11
10
10
10
9
9
9
9
9
6
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
695
325
271
250
221
208
168
121
61
40
17
14
11
8
6
4
2
2
1
1
Options
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Catalog No. ABIN391300
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0922565 ABIN2792242 WB Rabbit C-Term Log in to see Polyclonal 1
3.0922565 ABIN391298 IHC (p) WB Rabbit Ig AA 56-85, Ser78 Log in to see Polyclonal 2
3.0922565 ABIN391299 IHC (p) WB Rabbit Ig AA 60-89, Ser82 Log in to see Polyclonal 2
3.0922565 ABIN391296 WB Rabbit Ig AA 61-90, Ser83 Log in to see Polyclonal 2
3.0922565 ABIN317528 EIA IHC (p) WB Mouse IgG1 AA 1-205, N-Term Log in to see 2A5 3
3.0922565 ABIN361756 ICC IF IP IHC WB Mouse IgG1 kappa Log in to see 8A7 9
3.0922565 ABIN4620439 EIA IHC (p) WB Rabbit Ig Ser82 Log in to see Polyclonal 5
3.0922565 ABIN4620440 EIA WB Rabbit Ig Ser83 Log in to see Polyclonal 5
3.0922565 ABIN4620438 EIA IHC (p) WB Rabbit Ig Ser78 Log in to see Polyclonal 5
3.0922565 ABIN453119 EIA WB Rabbit Middle Region Log in to see Polyclonal 5
3.0922565 ABIN969199 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 5D7 1
3.0922565 ABIN1027725 ICC IF WB Rabbit Log in to see Polyclonal 7
3.0922565 ABIN4319980 ICC IF IHC IHC (p) WB Rabbit AA 80-150, Internal Region Log in to see Polyclonal 2
3.0922565 ABIN3042464 IHC (fro) IHC (p) WB Rabbit IgG AA 1-205 Log in to see Polyclonal
3.0922565 ABIN126812 IHC (fro) WB Mouse IgG2a pSer82 Log in to see 5B9
3.0922565 ABIN1558225 ICC WB Mouse IgG1 Log in to see 7E5-1F9-D9
3.0922565 ABIN1531867 ELISA WB Rabbit IgG pSer78 Log in to see Polyclonal
3.0922565 ABIN1531866 IHC ELISA WB Rabbit IgG pSer15 Log in to see Polyclonal
3.0922565 ABIN1531868 ELISA WB Rabbit IgG pSer82 Log in to see Polyclonal
3.0922565 ABIN933725 IHC ELISA WB Mouse IgG1 kappa AA 1-205 Log in to see 2A5 1

Top referenced anti-HSPB1 antibody for Western Blotting

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Application / Reactivity Human
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 8 Antibodies
Dot Blot (DB) 17 Antibodies
ELISA 259 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 14 Antibodies
Flow Cytometry (FACS) 121 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 202 Antibodies
Immunofluorescence (IF) 214 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 318 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 61 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope AA 108-136, Center
(78), (64), (51), (45), (24), (17), (16), (16), (15), (13), (12), (8), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(806), (281), (276), (87), (78), (72), (71), (51), (46), (31), (30), (26), (18), (13), (11), (9), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(485), (340), (3)
Clonality (Clone)
Polyclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(34), (34), (23), (20), (13), (13), (13), (13), (12), (11), (10), (10), (10), (9), (9), (9), (9), (9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(695), (325), (271), (250), (221), (208), (168), (121), (61), (40), (17), (14), (11), (8), (6), (4), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN391300) Images
Specificity This HSP27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115~145 amino acids from the Center region of human HSP27.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This HSP27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-136 AA from the Central region of human HSP27.
Clone RB19530
Isotype Ig

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 Antibody (ABIN391300) Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSBP1 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores, and high levels are associated with carcinoma of the breast and with endometrial adenocarcinomas. Heat shock of HeLa cell cultures, or treatment with arsenite, phorbol ester, or tumor necrosis factor, causes a rapid phosphorylation of preexisting HSBP1, with Ser82 as the major site and Ser78 the minor site of phosphorylation. HSBP1 may exert phosphorylation-activated functions linked with growth signaling pathways in unstressed cells. A homeostatic function at this level could protect cells from adverse effects of signal transduction systems which may be activated inappropriately during stress.
Synonyms: Heat shock protein beta-1, HspB1, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, Estrogen-regulated 24 kDa protein, 28 kDa heat shock protein, HSPB1, HSP27
Molecular Weight 22783 DA
Gene ID 3315
UniProt P04792
Research Area Apoptosis/Necrosis, Stem Cells, Protein Modifications, Cell Structure
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 Antibody (ABIN391300) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 Antibody (ABIN391300) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-HSPB1 Antibody (ABIN391300)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Background publications

Song, Ethier, Dziubinski, Lin: "Stat3 modulates heat shock 27kDa protein expression in breast epithelial cells." in: Biochemical and biophysical research communications, Vol. 314, Issue 1, pp. 143-50, 2004

Chauhan, Li, Hideshima, Podar, Mitsiades, Mitsiades, Catley, Tai, Hayashi, Shringarpure, Burger, Munshi, Ohtake, Saxena, Anderson: "Hsp27 inhibits release of mitochondrial protein Smac in multiple myeloma cells and confers dexamethasone resistance." in: Blood, Vol. 102, Issue 9, pp. 3379-86, 2003

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN391300) back to top
Supplier Images
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 108-136) (ABIN391300) Western blot analysis of HSP27 antibody (Center) (ABIN391300) in MCF-7 cell line lysa...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (AA 108-136) (ABIN391300) Hsp 27 Antibody (Center) (ABIN391300) IHC analysis in formalin fixed and paraffin emb...