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anti-Human HSPB1 antibody for Western Blotting

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Epitope
Ser83
64
63
50
22
22
16
15
13
12
11
8
6
5
5
5
5
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
608
151
137
41
37
28
25
22
18
16
9
8
4
3
2
2
1
1
1
1
Host
Rabbit
449
187
1
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
28
25
17
13
13
11
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
3
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
515
254
226
141
126
106
96
40
34
27
18
14
5
2
2
2
Supplier
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Catalog No. ABIN359387
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN359389 EIA IHC (p) WB Rabbit Ig Ser78 Log in to see Polyclonal 5
0.12303223 ABIN359390 EIA IHC (p) WB Rabbit Ig Ser82 Log in to see Polyclonal 5
0.12303223 ABIN391300 IHC (p) WB Rabbit Ig AA 108-136, Center Log in to see Polyclonal 2
0.12303223 ABIN317528 EIA IHC (p) WB Mouse IgG1 AA 1-205, N-Term Log in to see 2A5 3
0.12303223 ABIN453119 EIA WB Rabbit Middle Region Log in to see Polyclonal 5
0.12303223 ABIN2779293 IHC WB Rabbit C-Term Log in to see Polyclonal 2
0.12303223 ABIN2792242 WB Rabbit C-Term Log in to see Polyclonal 1
0.12303223 ABIN391296 WB Rabbit Ig AA 61-90, Ser83 Log in to see Polyclonal 2
0.12303223 ABIN391298 IHC (p) WB Rabbit Ig AA 56-85, Ser78 Log in to see Polyclonal 2
0.12303223 ABIN391299 IHC (p) WB Rabbit Ig AA 60-89, Ser82 Log in to see Polyclonal 2
0.12303223 ABIN263953 IHC (fro) IF IP WB Mouse IgG1 Log in to see 8A7 8
0.12303223 ABIN361756 ICC IF IP IHC WB Mouse Log in to see 8A7 9
0.12303223 ABIN969199 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 5D7 1
0.12303223 ABIN1027725 ICC IF WB Rabbit Log in to see Polyclonal 7
0.12303223 ABIN933725 IHC ELISA WB Mouse IgG1 kappa AA 1-205 Log in to see 2A5 1
0.12303223 ABIN126812 IHC (fro) WB Mouse IgG2a pSer82 Log in to see 5B9
0.12303223 ABIN1558225 ICC WB Mouse IgG1 Log in to see 7E5-1F9-D9
0.12303223 ABIN1531867 ELISA WB Rabbit IgG pSer78 Log in to see Polyclonal
0.12303223 ABIN1531866 IHC ELISA WB Rabbit IgG pSer15 Log in to see Polyclonal
0.12303223 ABIN1531868 ELISA WB Rabbit IgG pSer82 Log in to see Polyclonal

Top referenced anti-HSPB1 antibody for Western Blotting

Similar anti-HSPB1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 19 Antibodies
ELISA 215 Antibodies
Enzyme Immunoassay (EIA) 15 Antibodies
Flow Cytometry (FACS) 35 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 94 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies
Immunofluorescence (IF) 135 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 258 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 24 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 108 Antibodies
Immunoprecipitation (IP) 120 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope Ser83
(64), (63), (50), (22), (22), (16), (15), (13), (12), (11), (8), (6), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(608), (151), (137), (41), (37), (28), (25), (22), (18), (16), (9), (8), (4), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(449), (187), (1)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(28), (25), (17), (13), (13), (11), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(515), (254), (226), (141), (126), (106), (96), (40), (34), (27), (18), (14), (5), (2), (2), (2)
Pubmed 5 references available
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN359387) Images
Specificity This antibody detects HSP27.
Purification Protein A column, followed by peptide affinity purification.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S83 of human HSPB1.
Isotype Ig

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling References for anti-HSPB1 Antibody (ABIN359387) Images back to top
Antigen
Alternative Name HSPB1 / HSP27 (HSPB1 Antibody Abstract)
Background In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSBP1 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores, and high levels are associated with carcinoma of the breast and with endometrial adenocarcinomas. Heat shock of HeLa cell cultures, or treatment with arsenite, phorbol ester, or tumor necrosis factor, causes a rapid phosphorylation of preexisting HSBP1, with Ser82 as the major site and Ser78 the minor site of phosphorylation. HSBP1 may exert phosphorylation-activated functions linked with growth signaling pathways in unstressed cells. A homeostatic function at this level could protect cells from adverse effects of signal transduction systems which may be activated inappropriately during stress.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27
Molecular Weight 22783 Da.
Gene ID 3315, 9606
UniProt P04792
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling References for anti-HSPB1 Antibody (ABIN359387) Images back to top
Application Notes ELISA 1: 1,000. Western blot 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details References for anti-HSPB1 Antibody (ABIN359387) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-HSPB1 Antibody (ABIN359387)

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling Images back to top
Background publications

Wano, Kita, Takahashi, Sugaya, Hino, Hosoya, Suzuki: "Protective role of HSP27 against UVC-induced cell death in human cells." in: Experimental cell research, Vol. 298, Issue 2, pp. 584-92, 2004

Evgrafov, Mersiyanova, Irobi, Van Den Bosch, Dierick, Leung, Schagina, Verpoorten, Van Impe, Fedotov, Dadali, Auer-Grumbach, Windpassinger, Wagner, Mitrovic, Hilton-Jones, Talbot, Martin, Vasserman et al.: "Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. ..." in: Nature genetics, Vol. 36, Issue 6, pp. 602-6, 2004

Song, Ethier, Dziubinski, Lin: "Stat3 modulates heat shock 27kDa protein expression in breast epithelial cells." in: Biochemical and biophysical research communications, Vol. 314, Issue 1, pp. 143-50, 2004

Chauhan, Li, Hideshima, Podar, Mitsiades, Mitsiades, Catley, Tai, Hayashi, Shringarpure, Burger, Munshi, Ohtake, Saxena, Anderson: "Hsp27 inhibits release of mitochondrial protein Smac in multiple myeloma cells and confers dexamethasone resistance." in: Blood, Vol. 102, Issue 9, pp. 3379-86, 2003

Van Why, Mann, Ardito, Thulin, Ferris, Macleod, Kashgarian, Siegel: "Hsp27 associates with actin and limits injury in energy depleted renal epithelia." in: Journal of the American Society of Nephrology : JASN, Vol. 14, Issue 1, pp. 98-106, 2002

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling References for anti-HSPB1 Antibody (ABIN359387) back to top
Supplier Images
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Ser83) (ABIN359387) Western blot analysis of HSPB1 Antibody in Hela cell line lysates (35 µg/lane). HSPB1...
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Ser83) (ABIN359387) Western blot analysis of HSPB1(arrow) using rabbit polyclonal HSPB1 Antibody