|Application / Reactivity||Mammalian||Human|
|ELISA||1 Antibodies||261 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||11 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||202 Antibodies|
|Immunofluorescence (IF)||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||2 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|Western Blotting (WB)||2 Antibodies|
|Cell Culture (CC)||1 Antibodies|
|Cytometry by Time of Flight (CyTOF)||8 Antibodies|
|Dot Blot (DB)||17 Antibodies|
|ELISA (Detection)||1 Antibodies|
|Flow Cytometry (FACS)||156 Antibodies|
|Immunochromatography (IC)||5 Antibodies|
|Antigen||Heat Shock 27kDa Protein 1 (HSPB1) Antibodies|
|Reactivity||Human, Mammalian Alternatives|
|Conjugate||This HSPB1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-HSPB1 AntibodyTarget Details HSPB1 Application Details Handling Images
|Specificity||Prokaryotic the full length Human HSP27 protein. This antibody recognizes Heat Shock 27 kDa Protein 1 (HSPB1).|
Species reactivity (expected):Mammal.
Species reactivity (tested):Human.
|Characteristics||Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein,SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein|
|Purification||Chromatography on Protein A|
|Immunogen||Recombinant protein. Prokaryotic the full length Human HSP27 protein.|
Target Details HSPB1Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
|Alternative Name||HSPB1 / HSP27 (HSPB1 Antibody Abstract)|
|Background||Hsp27, also referred to as the Estrogen regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen responsive nature of Hsp27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27|
|Pathways||MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling|
Application DetailsProduct Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
ELISA. Immunocytochemistry. Western blot: 1/1000. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 1.25 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
|Buffer||0.02 M Potassium Phosphate, 0.2 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide, None|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top