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anti-Mammalian HSPB1 antibody for Western Blotting

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Reactivity
Human, Mammalian
608
151
137
41
37
28
25
22
18
16
9
8
4
3
2
1
1
1
1
1
Host
Mouse
450
186
1
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
28
25
17
13
13
11
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
3
2
2
2
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
515
254
226
140
125
105
96
40
34
27
18
14
5
2
2
2
Supplier
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Available images

Catalog No. ABIN400642
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011794439 ABIN1723427 ICC IHC (p) IP ELISA WB Mouse IgG1 lambda Log in to see

Similar anti-HSPB1 Antibodies

Application / Reactivity Mammalian Human
ELISA 1 Antibodies 215 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 15 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 94 Antibodies
Immunofluorescence (IF) 1 Antibodies 135 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 2 Antibodies
Dot Blot (DB) 19 Antibodies
Flow Cytometry (FACS) 35 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 258 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Human, Mammalian
(608), (151), (137), (41), (37), (28), (25), (22), (18), (16), (9), (8), (4), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(450), (186), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(28), (25), (17), (13), (13), (11), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(515), (254), (226), (140), (125), (105), (96), (40), (34), (27), (18), (14), (5), (2), (2), (2)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Specificity Prokaryotic the full length Human HSP27 protein. This antibody recognizes Heat Shock 27 kDa Protein 1 (HSPB1).
Cross-Reactivity (Details) Species reactivity (expected):Mammal.
Species reactivity (tested):Human.
Characteristics Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein,SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein
Purification Chromatography on Protein A
Immunogen Recombinant protein. Prokaryotic the full length Human HSP27 protein.
Clone N-a
Isotype IgG1

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSPB1 / HSP27 (HSPB1 Antibody Abstract)
Background Hsp27, also referred to as the Estrogen regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen responsive nature of Hsp27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27
Gene ID 3315
UniProt P04792
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes ELISA. Immunocytochemistry. Western blot: 1/1000. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 1.25 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.2 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN400642) anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody