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anti-Mammalian HSPB1 antibody for Western Blotting

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • cb153
  • cb660
  • hsp1
  • hsp25
  • hsp27
  • id:ibd2821
  • sb:cb660
  • zgc:103437
  • HSPB1
  • cmt2f
  • hs.76067
  • hsp28
  • CMT2F
  • HMN2B
  • HS.76067
  • HSP27
  • HSP28
  • Hsp25
  • SRP27
  • Hs.78846
  • LOH11CR1K
  • MKBP
  • Hsp27
  • 27kDa
  • 2810021G24Rik
  • heat shock protein, alpha-crystallin-related, 1
  • heat shock 27kDa protein 1
  • heat shock 27kDa protein 2
  • heat shock protein 1
  • heat shock protein 2
  • heat shock protein 25
  • hspb1
  • HSPB1
  • HSP27
  • HSPB2
  • Hspb1
  • Hspb2
  • HSP25
Reactivity
Human, Mammalian
811
282
276
87
78
72
71
51
46
31
30
26
18
13
11
9
6
4
3
2
2
1
1
1
1
1
Host
Mouse
491
339
3
Clonality
Monoclonal
Conjugate
This HSPB1 antibody is un-conjugated
34
34
23
20
13
13
13
13
12
11
10
10
10
9
9
9
9
9
6
5
5
5
5
5
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
700
326
271
253
220
208
167
121
61
40
17
13
11
8
6
4
2
2
1
1
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Catalog No. ABIN400642
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1723427 ICC IHC (p) IP ELISA WB Mouse IgG1 lambda Log in to see

Similar anti-HSPB1 Antibodies

Application / Reactivity Mammalian Human
ELISA 1 Antibodies 259 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 14 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 202 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 2 Antibodies
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 8 Antibodies
Dot Blot (DB) 17 Antibodies
ELISA (Detection) 1 Antibodies
Flow Cytometry (FACS) 121 Antibodies
Immunochromatography (IC) 5 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Reactivity Human, Mammalian
(811), (282), (276), (87), (78), (72), (71), (51), (46), (31), (30), (26), (18), (13), (11), (9), (6), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(491), (339), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HSPB1 antibody is un-conjugated
(34), (34), (23), (20), (13), (13), (13), (13), (12), (11), (10), (10), (10), (9), (9), (9), (9), (9), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(700), (326), (271), (253), (220), (208), (167), (121), (61), (40), (17), (13), (11), (8), (6), (4), (2), (2), (1), (1)
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Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Specificity Prokaryotic the full length Human HSP27 protein. This antibody recognizes Heat Shock 27 kDa Protein 1 (HSPB1).
Cross-Reactivity (Details) Species reactivity (expected):Mammal.
Species reactivity (tested):Human.
Characteristics Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein,SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein
Purification Chromatography on Protein A
Immunogen Recombinant protein. Prokaryotic the full length Human HSP27 protein.
Clone N-a
Isotype IgG1

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSPB1 / HSP27 (HSPB1 Antibody Abstract)
Background Hsp27, also referred to as the Estrogen regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen responsive nature of Hsp27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27
Gene ID 3315
UniProt P04792
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes ELISA. Immunocytochemistry. Western blot: 1/1000. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 1.25 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.2 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide, None
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (ABIN400642) anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody