You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) HSPB1 antibody for Flow Cytometry

Recommended HSPB1 Antibody (supplied by: Log in to see )

Antigen
Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
  • 27kDa
  • 2810021G24Rik
  • cb153
  • cb660
  • cmt2f
  • CMT2F
  • HMN2B
  • hs.76067
  • HS.76067
  • Hs.78846
  • hsp1
  • Hsp25
  • hsp25
  • Hsp27
  • HSP27
  • hsp27
  • hsp28
  • HSP28
  • HSPB1
  • id:ibd2821
  • LOH11CR1K
  • MKBP
  • sb:cb660
  • SRP27
  • zgc:103437
Epitope
Center
67
66
52
40
25
22
16
16
13
13
12
7
6
5
5
5
5
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
751
271
259
86
79
76
73
53
41
38
24
22
18
8
4
4
3
2
1
1
1
Host
Rabbit
479
311
3
Clonality
Polyclonal
Conjugate
This HSPB1 antibody is un-conjugated
32
31
23
17
13
13
11
10
10
9
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
667
289
266
237
228
187
183
127
68
34
18
15
5
2
2
2
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2854951
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010469276 ABIN4320066 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see HSPB1-774
0.0010469276 ABIN4320067 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see HSPB1-774
0.0010469276 ABIN4320068 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see HSPB1-774
0.0010469276 ABIN4320007 FACS ICC IF WB Mouse IgG1 Log in to see 6H11
0.0010469276 ABIN4320008 FACS ICC IF WB Mouse IgG1 Log in to see 6H11
0.0010469276 ABIN4320035 FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa Log in to see G3-1
0.0010469276 ABIN4320036 FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG1 kappa Log in to see G3-1
0.0010469276 ABIN4320037 FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 kappa Log in to see G3-1
0.0010469276 ABIN4320038 FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG1 kappa Log in to see G3-1
0.0010469276 ABIN4320039 FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 405LS Mouse IgG1 kappa Log in to see G3-1
0.0010469276 ABIN4320040 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see SPM252
0.0010469276 ABIN4320041 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see SPM252
0.0010469276 ABIN4320042 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 kappa Log in to see SPM252
0.0010469276 ABIN4320043 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see SPM252
0.0010469276 ABIN4320044 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Allophycocyanin Mouse IgG1 kappa Log in to see SPM252
0.0010469276 ABIN4320045 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 kappa Log in to see SPM252
0.0010469276 ABIN4320046 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa Log in to see SPM252
0.0010469276 ABIN4320047 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 kappa Log in to see SPM252
0.0010469276 ABIN4320048 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see SPM252
0.0010469276 ABIN4320050 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see SPM252

Similar anti-HSPB1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 42 Antibodies 39 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 2 Antibodies
Flow Cytometry (FACS) 101 Antibodies 99 Antibodies
Immunocytochemistry (ICC) 123 Antibodies 136 Antibodies
Immunoelectron Microscopy (IEM) 2 Antibodies 2 Antibodies
Immunofluorescence (IF) 145 Antibodies 156 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 90 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 49 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 125 Antibodies
Immunoprecipitation (IP) 82 Antibodies
Western Blotting (WB) 225 Antibodies

General

Antigen Heat Shock 27kDa Protein 1 (HSPB1) Antibodies
Epitope Center
(67), (66), (52), (40), (25), (22), (16), (16), (13), (13), (12), (7), (6), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(751), (271), (259), (86), (79), (76), (73), (53), (41), (38), (24), (22), (18), (8), (4), (4), (3), (2), (1), (1), (1)
Host Rabbit
(479), (311), (3)
Clonality
Polyclonal
Conjugate This HSPB1 antibody is un-conjugated
(32), (31), (23), (17), (13), (13), (11), (10), (10), (9), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(667), (289), (266), (237), (228), (187), (183), (127), (68), (34), (18), (15), (5), (2), (2), (2)
Supplier Log in to see

Product Details anti-HSPB1 Antibody

Target Details HSPB1 Application Details Handling Images
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (100 %)
Characteristics Rabbit Polyclonal antibody to HSP27 (heat shock 27 kDa protein 1)
HSP27 antibody
Purification Affinity purified by Protein A.
Immunogen Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.
Isotype IgG

Target Details HSPB1

Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).

Cellular Localization: Cytoplasm , Nucleus , Spindle
Molecular Weight 23 kDa
Gene ID 3315
Research Area Heat Shock Proteins
Pathways MAPK Signaling

Application Details

Product Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Application Notes Suggested dilution Reference Flow cytometry/FACS Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceFlow cytometry/FACSAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:1000* Western blot1:500-1:10000*
Comment

Positive Control: A431 , HeLa , mouse liver , *HT29

Restrictions For Research Use only

Handling

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
Format Liquid
Concentration 3.23 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Center) (ABIN2854951) WB Image Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE antibody dil...
Immunohistochemistry (IHC) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Center) (ABIN2854951) IHC-P Image HSP27 antibody detects HSPB1 protein at cytosol on U87 xenograft by immun...
Immunoprecipitation (IP) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Center) (ABIN2854951) IP Image Immunoprecipitation of HSP27 protein from HeLa whole cell extracts using 5 μ...
Western Blotting (WB) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Center) (ABIN2854951) WB Image HSP27 antibody detects HSP27 protein by western blot analysis. A. 30 μg A431...
Immunofluorescence (IF) image for anti-HSPB1 antibody (Heat Shock 27kDa Protein 1) (Center) (ABIN2854951) ICC/IF Image HSP27 antibody detects HSP27 protein at cytoplasm and cytoskeleton by im...