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|Application / Reactivity||Human||Rat (Rattus)||Mouse (Murine)||Dog (Canine)|
|Immunofluorescence (fixed cells) (IF/ICC)||9 Antibodies||4 Antibodies||3 Antibodies|
|Cytometry by Time of Flight (CyTOF)||8 Antibodies||8 Antibodies||8 Antibodies|
|Simple Western (SimWes)||4 Antibodies|
|Western Blotting (WB)||677 Antibodies||222 Antibodies||46 Antibodies||225 Antibodies|
|Radioimmunoassay (RIA)||2 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Immunoprecipitation (IP)||159 Antibodies||59 Antibodies||63 Antibodies||27 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||252 Antibodies||114 Antibodies|
|Antigen||Heat Shock 27kDa Protein 1 (HSPB1) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This HSPB1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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Product Details anti-HSPB1 AntibodyTarget Details HSPB1 Application Details Handling Images
|Specificity||This antibody specifically interacts with HSP27 phosphorylated at Serine 82 and does not crossreact with the non-phosphorylated HSP27 nor with unrelated Serine-phosphorylated proteins.|
|Characteristics||Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein,SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein|
|Purification||Size Exclusion Chromatography|
|Components||incl. positive Control|
|Immunogen||Synthetic phosphopeptide conjugated to KLH.|
Target Details HSPB1Product Details anti-HSPB1 Antibody Application Details Handling Images back to top
|Alternative Name||HSPB1 / HSP27 (HSPB1 Antibody Abstract)|
|Background||The small heat shock protein hsp27 is constitutively expressed in most cell lines. The expression level is increased in response to environmental stress. Activation of the p38 kinases results in subsequent activation of MAPKAP2 that phosphorylates hsp27 at Ser15, Ser78 and Ser82.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27|
|Pathways||MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling|
Application DetailsProduct Details anti-HSPB1 Antibody Target Details HSPB1 Handling Images back to top
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from EGF-treated HepG2 cellsImmunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Images back to top
|Reconstitution||Restore with 1 mL H2O (15 min, RT).|
|Buffer||1 mLPBS containing 0.09 % Sodium Azide, PEG and Sucrose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C/-80 °C|
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-HSPB1 Antibody Target Details HSPB1 Application Details Handling back to top